Analytical Biochemistry

, том 491 , страницы 65-71

Application of gold nanoparticles produced by laser ablation for immunochromatographic assay labeling

Тип публикации: Journal Article

Дата публикации: 2015-12-01

Elsevier

Analytical Biochemistry

SCImago Q3

WOS Q2

БС2

SJR: 0.487

CiteScore: 5.9

Impact factor: 3.1

ISSN: 00032697, 10960309

DOI: 10.1016/j.ab.2015.08.031

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PubMed ID: 26391846

Biochemistry

Molecular Biology

Cell Biology

Biophysics

Краткое описание

Nanodispersed gold is widely used as a marker in different analytical systems. For such purposes, it is usually obtained by the reduction of salts. This work studied the potential analytical applications of nanodispersed gold obtained by laser ablation because gold produced with this method has no chemical coating. The nanoparticles produced were characterized by transmission electron microscopy and spectrophotometry. The average size of the particles was 24.5 nm. Concentration dependences of antibody immobilization on ablative gold were obtained. With the use of antibody-conjugated nanoparticles, an immunochromatographic system was constructed for the detection of zearalenone mycotoxin. This immunoassay was characterized by a detection limit of 0.1 ng/ml antigen with an assay duration of only 15 min, which is on par with current test systems comprising nanodispersed gold obtained by chemical reduction. The simplicity of ablative dispersing makes this a prospective method for the labeling of various antibodies for analytical use.

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Urusov A. E. et al. Application of gold nanoparticles produced by laser ablation for immunochromatographic assay labeling // Analytical Biochemistry. 2015. Vol. 491. pp. 65-71.

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Urusov A. E., Petrakova A. V., Kuzmin P. G., Zherdev A. V., Sveshnikov P. G., Shafeev G. A., Dzantiev B. B. Application of gold nanoparticles produced by laser ablation for immunochromatographic assay labeling // Analytical Biochemistry. 2015. Vol. 491. pp. 65-71.

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TY - JOUR

DO - 10.1016/j.ab.2015.08.031

UR - https://doi.org/10.1016/j.ab.2015.08.031

TI - Application of gold nanoparticles produced by laser ablation for immunochromatographic assay labeling

T2 - Analytical Biochemistry

AU - Urusov, A E

AU - Petrakova, Alina V.

AU - Kuzmin, P G

AU - Zherdev, Anatoly V.

AU - Sveshnikov, P. G.

AU - Shafeev, G A

AU - Dzantiev, Boris B.

PY - 2015

DA - 2015/12/01

PB - Elsevier

SP - 65-71

VL - 491

PMID - 26391846

SN - 0003-2697

SN - 1096-0309

ER -

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@article{2015_Urusov,

author = {A E Urusov and Alina V. Petrakova and P G Kuzmin and Anatoly V. Zherdev and P. G. Sveshnikov and G A Shafeev and Boris B. Dzantiev},

title = {Application of gold nanoparticles produced by laser ablation for immunochromatographic assay labeling},

journal = {Analytical Biochemistry},

year = {2015},

volume = {491},

publisher = {Elsevier},

month = {dec},

url = {https://doi.org/10.1016/j.ab.2015.08.031},

pages = {65--71},

doi = {10.1016/j.ab.2015.08.031}

}

Издатель

Elsevier

Журнал

Analytical Biochemistry

SCImago Q3

WOS Q2

БС2

SJR

0.487

CiteScore

5.9

Impact factor

3.1

ISSN

00032697 (Print)

10960309 (Electronic)

Лаборатории

Лаборатория иммунобиохимии

Профили

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