RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function
Тип публикации: Journal Article
Дата публикации: 2006-06-24
PubMed ID:
16793519
Drug Discovery
Biochemistry
Molecular Biology
General Medicine
Pharmacology
Clinical Biochemistry
Molecular Medicine
Краткое описание
The higher-order structural organization of the cell nucleus reflects the underlying genome-wide transcriptional activity and macromolecular transport processes. To study the microscopic organization of RNA distribution within the nucleus, a combinatorial library of fluorescent styryl molecules was synthesized and screened for an in vitro RNA response and live cell nuclear imaging. Four different cell lines (HeLa, A549, 3T3, and 3T3-L1) were analyzed in terms of higher-order nuclear organization. We identified RNA-selective dyes with better imaging properties relative to commercially available SYTORNASelect dye; the selected dyes were also cell permeant, photostable, and well tolerated by the cells. Our dyes also had very good counterstain compatibility with Hoechst and DAPI, which could help to image the DNA distribution in relation to RNA distribution in live cells and therefore reveal different patterns of RNA-DNA colocalization.
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194
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194
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MLA
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ГОСТ
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Li Q. et al. RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function // Chemistry & Biology. 2006. Vol. 13. No. 6. pp. 615-623.
ГОСТ со всеми авторами (до 50)
Скопировать
Li Q., Kim Y., Namm J., Kulkarni A., Rosania G. R., Ahn Y., Chang Y. D. RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function // Chemistry & Biology. 2006. Vol. 13. No. 6. pp. 615-623.
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RIS
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TY - JOUR
DO - 10.1016/j.chembiol.2006.04.007
UR - https://doi.org/10.1016/j.chembiol.2006.04.007
TI - RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function
T2 - Chemistry & Biology
AU - Li, Qian
AU - Kim, Yun‑Kyung
AU - Namm, Joshua
AU - Kulkarni, Amita
AU - Rosania, Gus R.
AU - Ahn, Y
AU - Chang, Y D
PY - 2006
DA - 2006/06/24
PB - Elsevier
SP - 615-623
IS - 6
VL - 13
PMID - 16793519
SN - 1074-5521
SN - 1879-1301
ER -
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BibTex (до 50 авторов)
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@article{2006_Li,
author = {Qian Li and Yun‑Kyung Kim and Joshua Namm and Amita Kulkarni and Gus R. Rosania and Y Ahn and Y D Chang},
title = {RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function},
journal = {Chemistry & Biology},
year = {2006},
volume = {13},
publisher = {Elsevier},
month = {jun},
url = {https://doi.org/10.1016/j.chembiol.2006.04.007},
number = {6},
pages = {615--623},
doi = {10.1016/j.chembiol.2006.04.007}
}
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MLA
Скопировать
Li, Qian, et al. “RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function.” Chemistry & Biology, vol. 13, no. 6, Jun. 2006, pp. 615-623. https://doi.org/10.1016/j.chembiol.2006.04.007.
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