, volume 45 , issue 5 , pages 874-880

Wilms’ tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic cancer cells

Wolfgang Glienke ¹

Luise Maute ²

Johannes Wicht ²

L. Bergmann ²

Hide authors affiliations Show authors affiliations: 2 affiliations

Department of Medicine II (Hämatologie/Onkologie), University Hospital, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany. |

Department of Medicine II (Hämatologie/Onkologie), University Hospital, Johann Wolfgang Goethe-University, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany |

Publication type: Journal Article

Publication date: 2009-03-01

Elsevier

European Journal of Cancer

scimago Q1

wos Q1

SJR: 2.691

CiteScore: 11.4

Impact factor: 7.1

ISSN: 09598049, 18790852

DOI: 10.1016/j.ejca.2008.12.030

Copy DOI

PubMed ID: 19196508

Cancer Research

Oncology

Abstract

The transcription factor WT1 plays an important role in cellular proliferation and survival of various cancer cells, and is frequently expressed in pancreatic cancer. Curcumin has been shown to be a potentially effective agent in pancreatic cancer. In this context, the purpose of this study was to determine the role of WT1 in a curcumin-treated pancreatic cancer cell line. To study the effect of curcumin on the expression of WT1, we incubated the pancreatic cancer cell line PANC-1 with different amounts of curcumin. The expression of WT1 on mRNA and protein level was measured with real-time RT-PCR and Western blot analysis. The incubation of the pancreatic cancer cell line PANC-1 with curcumin resulted in an inhibition of cellular proliferation as measured with MTT assay. The expression of WT1 on mRNA and protein level was significantly down-regulated in a concentration-dependent manner after treatment with curcumin. The WT1 mRNA levels were decreased by 20%, 25%, 40%, 78% and 88% in response to 10, 20, 30, 40 and 50 microM curcumin. The use of small inhibitory RNA (siRNA) targeting WT1 down-regulated the expression of WT1 about 90%. Combined treatment with curcumin and siRNA targeting WT1 resulted in a significant inhibition of cell proliferation compared to curcumin-treated cells alone. In conclusion, WT1 is involved in cellular proliferation of PANC-1 cells. Targeting WT1 gene expression with siRNA may enhance the efficacy of curcumin to inhibit cell proliferation.

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GOST |

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Glienke W. et al. Wilms’ tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic cancer cells // European Journal of Cancer. 2009. Vol. 45. No. 5. pp. 874-880.

GOST all authors (up to 50) Copy

Glienke W., Maute L., Wicht J., Bergmann L. Wilms’ tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic cancer cells // European Journal of Cancer. 2009. Vol. 45. No. 5. pp. 874-880.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.ejca.2008.12.030

UR - https://doi.org/10.1016/j.ejca.2008.12.030

TI - Wilms’ tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic cancer cells

T2 - European Journal of Cancer

AU - Glienke, Wolfgang

AU - Maute, Luise

AU - Wicht, Johannes

AU - Bergmann, L.

PY - 2009

DA - 2009/03/01

PB - Elsevier

SP - 874-880

IS - 5

VL - 45

PMID - 19196508

SN - 0959-8049

SN - 1879-0852

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2009_Glienke,

author = {Wolfgang Glienke and Luise Maute and Johannes Wicht and L. Bergmann},

title = {Wilms’ tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic cancer cells},

journal = {European Journal of Cancer},

year = {2009},

volume = {45},

publisher = {Elsevier},

month = {mar},

url = {https://doi.org/10.1016/j.ejca.2008.12.030},

number = {5},

pages = {874--880},

doi = {10.1016/j.ejca.2008.12.030}

}

MLA

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MLA Copy

Glienke, Wolfgang, et al. “Wilms’ tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic cancer cells.” European Journal of Cancer, vol. 45, no. 5, Mar. 2009, pp. 874-880. https://doi.org/10.1016/j.ejca.2008.12.030.

Publisher

Elsevier

Journal

European Journal of Cancer

scimago Q1

wos Q1

SJR

2.691

CiteScore

11.4

Impact factor

7.1

ISSN

09598049 (Print)

18790852 (Electronic)