Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM
Publication type: Journal Article
Publication date: 2016-09-01
scimago Q1
wos Q1
SJR: 1.076
CiteScore: 10.2
Impact factor: 6.3
ISSN: 00143057, 18731945
Materials Chemistry
Organic Chemistry
General Physics and Astronomy
Polymers and Plastics
Abstract
The structural characterisation of high water content materials such as hydrogels is challenging due to the inherent properties of water that are dependent on conditions of temperature and pressure. Cryo-scanning electron microscopy (cryo-SEM) has potential to offer the most authentic insights into the native structure of polysaccharide hydrogels, due to the ability to freeze the sample under conditions where transparent vitreous ice is formed. The advantages, challenges and constraints of cryo-SEM in characterising the porous matrix of alginate hydrogels are considered in this tutorial paper through examples from our own studies. Recommendations are proposed for the minimum methodological information that is required from researchers as part of the publication of their studies of hydrogel samples to enable critical appraisal of their cryo-SEM images.
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Aston R. et al. Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM // European Polymer Journal. 2016. Vol. 82. pp. 1-15.
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Aston R., Sewell K., Klein T., Lawrie G., Grøndahl L. Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM // European Polymer Journal. 2016. Vol. 82. pp. 1-15.
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TY - JOUR
DO - 10.1016/j.eurpolymj.2016.06.025
UR - https://doi.org/10.1016/j.eurpolymj.2016.06.025
TI - Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM
T2 - European Polymer Journal
AU - Aston, Robyn
AU - Sewell, Kim
AU - Klein, Travis
AU - Lawrie, Gwen
AU - Grøndahl, L.
PY - 2016
DA - 2016/09/01
PB - Elsevier
SP - 1-15
VL - 82
SN - 0014-3057
SN - 1873-1945
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2016_Aston,
author = {Robyn Aston and Kim Sewell and Travis Klein and Gwen Lawrie and L. Grøndahl},
title = {Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM},
journal = {European Polymer Journal},
year = {2016},
volume = {82},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016/j.eurpolymj.2016.06.025},
pages = {1--15},
doi = {10.1016/j.eurpolymj.2016.06.025}
}