Free Radical Biology and Medicine, volume 196, pages 133-144
SERS uncovers the link between conformation of cytochrome c heme and mitochondrial membrane potential
Brazhe Nadezda A.
1
,
Grivennikova Vera G.
2
,
Semenova A. A.
3
,
Novikov Sergey
4
,
Volkov Valentyn S.
4, 5
,
Arsenin Aleksey
4
,
Evlyukhin Andrey
6
,
Bochkova Zh. V.
1
,
Goodilin Eugene A.
3, 7
,
Maksimov Georgy V.
1, 8
,
Sosnovtseva Olga V.
9
,
Rubin Andrei B
1
4
5
GrapheneTek, Skolkovo Innovation Center, Moscow, 121205, Russia
|
6
Institute of Quantum Optics, Leibniz Universität Hannover, Hannover, 30167, Germany
|
Publication type: Journal Article
Publication date: 2023-02-01
Journal:
Free Radical Biology and Medicine
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 7.4
ISSN: 08915849, 18734596
Biochemistry
Physiology (medical)
Abstract
The balance between the mitochondrial respiratory chain activity and the cell's needs in ATP ensures optimal cellular function. Cytochrome c is an essential component of the electron transport chain (ETC), which regulates ETC activity, oxygen consumption, ATP synthesis and can initiate apoptosis. The impact of conformational changes in cytochrome c on its function is not understood for the lack of access to these changes in intact mitochondria. We have developed a novel sensor that uses unique properties of label-free surface-enhanced Raman spectroscopy (SERS) to identify conformational changes in heme of cytochrome c and to elucidate their role in functioning mitochondria. We have verified that molecule bond vibrations assessed by SERS are a reliable indicator of the heme conformation during changes in the inner mitochondrial membrane potential and ETC activity. We have demonstrated that cytochrome c heme reversibly switches between planar and ruffled conformations in response to the inner mitochondrial membrane potential (ΔΨ) and H+ concentration in the intermembrane space. This regulates the efficiency of the mitochondrial respiratory chain, thus, adjusting the mitochondrial respiration to the cell's consumption of ATP and the overall activity. We have found that under hypertensive conditions cytochrome c heme loses its sensitivity to ΔΨ that can affect the regulation of ETC activity. The ability of the proposed SERS-based sensor to track mitochondrial function opens broad perspectives in cell bioenergetics.
Citations by journals
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1
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Molecules
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Molecules
1 publication, 16.67%
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Nanomaterials
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Nanomaterials
1 publication, 16.67%
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Journal of the American Chemical Society
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Journal of the American Chemical Society
1 publication, 16.67%
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ACS Sensors
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ACS Sensors
1 publication, 16.67%
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Heliyon
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Heliyon
1 publication, 16.67%
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Free Radical Biology and Medicine
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Free Radical Biology and Medicine
1 publication, 16.67%
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1
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Citations by publishers
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1
2
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 33.33%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
2 publications, 33.33%
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Elsevier
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Elsevier
2 publications, 33.33%
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1
2
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Brazhe N. A. et al. SERS uncovers the link between conformation of cytochrome c heme and mitochondrial membrane potential // Free Radical Biology and Medicine. 2023. Vol. 196. pp. 133-144.
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Brazhe N. A., Nikelshparg E. I., Baizhumanov A. A., Grivennikova V. G., Semenova A. A., Novikov S., Volkov V. S., Arsenin A., Yakubovsky D. I., Evlyukhin A., Bochkova Z. V., Goodilin E. A., Maksimov G. V., Sosnovtseva O. V., Rubin A. B. SERS uncovers the link between conformation of cytochrome c heme and mitochondrial membrane potential // Free Radical Biology and Medicine. 2023. Vol. 196. pp. 133-144.
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TY - JOUR
DO - 10.1016/j.freeradbiomed.2023.01.013
UR - https://doi.org/10.1016%2Fj.freeradbiomed.2023.01.013
TI - SERS uncovers the link between conformation of cytochrome c heme and mitochondrial membrane potential
T2 - Free Radical Biology and Medicine
AU - Brazhe, Nadezda A.
AU - Nikelshparg, Evelina I
AU - Baizhumanov, Adil A.
AU - Grivennikova, Vera G.
AU - Semenova, A. A.
AU - Novikov, Sergey
AU - Volkov, Valentyn S.
AU - Arsenin, Aleksey
AU - Yakubovsky, Dmitry I.
AU - Evlyukhin, Andrey
AU - Bochkova, Zh. V.
AU - Goodilin, Eugene A.
AU - Maksimov, Georgy V.
AU - Sosnovtseva, Olga V.
AU - Rubin, Andrei B
PY - 2023
DA - 2023/02/01 00:00:00
PB - Elsevier
SP - 133-144
VL - 196
SN - 0891-5849
SN - 1873-4596
ER -
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@article{2023_Brazhe,
author = {Nadezda A. Brazhe and Evelina I Nikelshparg and Adil A. Baizhumanov and Vera G. Grivennikova and A. A. Semenova and Sergey Novikov and Valentyn S. Volkov and Aleksey Arsenin and Dmitry I. Yakubovsky and Andrey Evlyukhin and Zh. V. Bochkova and Eugene A. Goodilin and Georgy V. Maksimov and Olga V. Sosnovtseva and Andrei B Rubin},
title = {SERS uncovers the link between conformation of cytochrome c heme and mitochondrial membrane potential},
journal = {Free Radical Biology and Medicine},
year = {2023},
volume = {196},
publisher = {Elsevier},
month = {feb},
url = {https://doi.org/10.1016%2Fj.freeradbiomed.2023.01.013},
pages = {133--144},
doi = {10.1016/j.freeradbiomed.2023.01.013}
}