Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase
Publication type: Journal Article
Publication date: 2009-04-01
SJR: —
CiteScore: —
Impact factor: —
ISSN: 13811177
Catalysis
Biochemistry
Process Chemistry and Technology
Bioengineering
Abstract
The microstructure and the catalytic properties of cross-linked enzyme aggregates (CLEA) of penicillin acylase (PA) obtained under different conditions were investigated. The period of time left between the enzyme precipitation and the cross-linking step was found to influence the structural organization of the resulting enzyme preparation. Confocal fluorescent microscopy of the so-called “fresh” and “mature” CLEAs PA allowed to estimate the “characteristic” diameter of CLEA PA particles, which appeared to be about 1.6 μm, and revealed that the “mature” type was composed of relatively big particles as compared to the “fresh” type. Complementary kinetic studies showed that the “mature” CLEA PA were more effective in both hydrolytic and synthetic reactions. It was suggested that the aggregate size might regulate the extent of covalent modification of PA and thereby influence the catalytic properties of CLEA.
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Pchelintsev N. A., Youshko M. I., Švedas V. K. Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase // Journal of Molecular Catalysis B Enzymatic. 2009. Vol. 56. No. 4. pp. 202-207.
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Pchelintsev N. A., Youshko M. I., Švedas V. K. Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase // Journal of Molecular Catalysis B Enzymatic. 2009. Vol. 56. No. 4. pp. 202-207.
Cite this
RIS
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TY - JOUR
DO - 10.1016/j.molcatb.2008.05.006
UR - https://doi.org/10.1016/j.molcatb.2008.05.006
TI - Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase
T2 - Journal of Molecular Catalysis B Enzymatic
AU - Pchelintsev, N A
AU - Youshko, M I
AU - Švedas, Vytas K.
PY - 2009
DA - 2009/04/01
PB - Elsevier
SP - 202-207
IS - 4
VL - 56
SN - 1381-1177
ER -
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BibTex (up to 50 authors)
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@article{2009_Pchelintsev,
author = {N A Pchelintsev and M I Youshko and Vytas K. Švedas},
title = {Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase},
journal = {Journal of Molecular Catalysis B Enzymatic},
year = {2009},
volume = {56},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016/j.molcatb.2008.05.006},
number = {4},
pages = {202--207},
doi = {10.1016/j.molcatb.2008.05.006}
}
Cite this
MLA
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Pchelintsev, N. A., et al. “Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase.” Journal of Molecular Catalysis B Enzymatic, vol. 56, no. 4, Apr. 2009, pp. 202-207. https://doi.org/10.1016/j.molcatb.2008.05.006.