Microbial reduction of ethyl acetoacetate to ethyl (R)-3-hydroxybutyrate in an ionic liquid containing system

A hydrophilic ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM]BF4) was successfully employed as co-solvent for asymmetric bioreduction of ethyl acetoacetate (EOB) to ethyl (R)-3-hydroxybutyrate (R-EHB) catalyzed by Pichia membranaefaciens Hansen ZJPH07 cells. The results demonstrated that the addition of [BMIM]BF4 in reaction system can markedly reduce the substrate inhibition and moderately improve the enantioselectivity compared to that in monophasic aqueous system. Among different alcohols and carbohydrates tried as co-substrate, glucose was a proper electron donor. Although isopropanol gave the best enantioselectivity with the highest yield, S-enantiomer was obtained. To optimize the bioreduction, some reaction parameters for the biosynthesis of R-EHB in this IL-containing system were investigated, such as temperature, buffer pH, shaking speed, substrate concentration, wet cells concentration and reaction time. Under the optimum conditions, best conversion of 77.8% and product enantiomeric excess (e.e.) of 73.0% were obtained. A comparative study was performed either in the presence or in the absence of [BMIM]BF4, higher reaction yield (77.8% versus 68.5%) and product e.e. (73.0% versus 65.1%) were observed in IL-containing system with 0.55 M of the substrate, but 0.35 M of substrate concentration for the reduction in aqueous system without the addition of [BMIM]BF4.