Open Access
Redox Biology, volume 50, pages 102245
Resistance to H2O2-induced oxidative stress in human cells of different phenotypes
Valeriy Zenin
1
,
Julia Ivanova
1
,
Natalia Pugovkina
1
,
Alla Shatrova
1
,
Nikolay Aksenov
1
,
Irina Tyuryaeva
1
,
Kseniya Kirpichnikova
1
,
Ivan Kuneev
2, 3
,
Andrei Zhuravlev
3, 4
,
Ekaterina Osyaeva
3, 4
,
Ekaterina Lyublinskaya
2, 3
,
Ilyuza Gazizova
2, 3
,
Nikita Guriev
2, 3
,
Olga Lyublinskaya
Publication type: Journal Article
Publication date: 2022-04-01
PubMed ID:
35114579
Organic Chemistry
Biochemistry
Clinical Biochemistry
Abstract
Application of genetically encoded biosensors of redox-active compounds promotes the elaboration of new methods for investigation of intracellular redox activities. Previously, we have developed a method to measure quantitatively the intracellular concentration of hydrogen peroxide (H2O2) in living cells using genetically encoded biosensor HyPer. In the present study, we refined the method and applied it for comparing the antioxidant system potency in human cells of different phenotypes by measuring the gradient between the extracellular and cytoplasmic H2O2 concentrations under conditions of H2O2-induced external oxidative stress. The measurements were performed using cancer cell lines (K-562 and HeLa), as well as normal human cells - all expressing HyPer in the cell cytoplasm. As normal cells, we used three isogenic lines of different phenotypes - mesenchymal stem/stromal cells (MSCs), induced pluripotent stem cells (iPSCs) derived from MSCs by reprogramming, and differentiated iPSC progenies with the phenotype resembling precursory MSCs. When exposing cells to exogenous H2O2, we showed that at low oxidative loads (<50 μM of H2O2) the gradient depended on extracellular H2O2 concentration. At high loads (>50 μM of H2O2), which caused the exhaustion of thioredoxin activity in the cell cytoplasm, the gradient stabilized, pointing out that it is the functional status of the thioredoxin-depended enzymatic system that drives the dependence of the H2O2 gradient on the oxidative load in human cells. At high H2O2 concentrations, the cytoplasmic H2O2 level in cancer cells was found to be several hundred times lower than the extracellular one. At the same time, in normal cells, extracellular-to-intracellular gradient amounted to thousands of times. Upon reprogramming, the potency of cellular antioxidant defense increased. In contrast, differentiation of iPSCs did not result in the changes in antioxidant system activity in the cell cytoplasm, assuming that intensification of the H2O2-detoxification processes is inherent to a period of early human development.
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Zenin V. et al. Resistance to H2O2-induced oxidative stress in human cells of different phenotypes // Redox Biology. 2022. Vol. 50. p. 102245.
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Zenin V., Ivanova J., Pugovkina N., Shatrova A., Aksenov N., Tyuryaeva I., Kirpichnikova K., Kuneev I., Zhuravlev A., Osyaeva E., Lyublinskaya E., Gazizova I., Guriev N., Lyublinskaya O., Lyublinskaya O. G. Resistance to H2O2-induced oxidative stress in human cells of different phenotypes // Redox Biology. 2022. Vol. 50. p. 102245.
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TY - JOUR
DO - 10.1016/j.redox.2022.102245
UR - https://doi.org/10.1016/j.redox.2022.102245
TI - Resistance to H2O2-induced oxidative stress in human cells of different phenotypes
T2 - Redox Biology
AU - Zenin, Valeriy
AU - Ivanova, Julia
AU - Pugovkina, Natalia
AU - Shatrova, Alla
AU - Aksenov, Nikolay
AU - Tyuryaeva, Irina
AU - Kirpichnikova, Kseniya
AU - Kuneev, Ivan
AU - Zhuravlev, Andrei
AU - Osyaeva, Ekaterina
AU - Lyublinskaya, Ekaterina
AU - Gazizova, Ilyuza
AU - Guriev, Nikita
AU - Lyublinskaya, Olga
AU - Lyublinskaya, Olga G.
PY - 2022
DA - 2022/04/01 00:00:00
PB - Elsevier
SP - 102245
VL - 50
PMID - 35114579
SN - 2213-2317
ER -
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@article{2022_Zenin,
author = {Valeriy Zenin and Julia Ivanova and Natalia Pugovkina and Alla Shatrova and Nikolay Aksenov and Irina Tyuryaeva and Kseniya Kirpichnikova and Ivan Kuneev and Andrei Zhuravlev and Ekaterina Osyaeva and Ekaterina Lyublinskaya and Ilyuza Gazizova and Nikita Guriev and Olga Lyublinskaya and Olga G. Lyublinskaya},
title = {Resistance to H2O2-induced oxidative stress in human cells of different phenotypes},
journal = {Redox Biology},
year = {2022},
volume = {50},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016/j.redox.2022.102245},
pages = {102245},
doi = {10.1016/j.redox.2022.102245}
}