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volume 4 issue 3 pages 102512

Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics

Publication typeJournal Article
Publication date2023-09-01
scimago Q1
wos Q4
SJR0.925
CiteScore2.5
Impact factor1.3
ISSN26661667
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience
Abstract
Antimicrobial susceptibility testing is used to determine the minimum inhibitory concentration (MIC), the standard measurement of antibiotic activity. Here, we present a protocol for evaluating MIC values of clinically relevant antibiotics against bacterial isolates cultured in standard bacteriologic medium and in mammalian cell culture medium. We describe steps for pathogen identification, culturing bacteria, preparing MIC plates, MIC assay incubation, and determining MIC. This protocol can potentially optimize the use of existing antibiotics while enhancing efforts to discover new ones. For complete details on the use and execution of this protocol, please refer to Heithoff et al.1.
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Cite this
GOST |
Cite this
GOST Copy
Barnes V L. et al. Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics // STAR Protocols. 2023. Vol. 4. No. 3. p. 102512.
GOST all authors (up to 50) Copy
Barnes V L., Heithoff D. M., Mahan S. P., House J., Mahan M. J. Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics // STAR Protocols. 2023. Vol. 4. No. 3. p. 102512.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/j.xpro.2023.102512
UR - https://doi.org/10.1016/j.xpro.2023.102512
TI - Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics
T2 - STAR Protocols
AU - Barnes V, Lucien
AU - Heithoff, Douglas M
AU - Mahan, Scott P
AU - House, J.K.
AU - Mahan, Michael J
PY - 2023
DA - 2023/09/01
PB - Elsevier
SP - 102512
IS - 3
VL - 4
PMID - 37566547
SN - 2666-1667
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2023_Barnes V,
author = {Lucien Barnes V and Douglas M Heithoff and Scott P Mahan and J.K. House and Michael J Mahan},
title = {Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics},
journal = {STAR Protocols},
year = {2023},
volume = {4},
publisher = {Elsevier},
month = {sep},
url = {https://doi.org/10.1016/j.xpro.2023.102512},
number = {3},
pages = {102512},
doi = {10.1016/j.xpro.2023.102512}
}
MLA
Cite this
MLA Copy
Barnes V., Lucien, et al. “Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics.” STAR Protocols, vol. 4, no. 3, Sep. 2023, p. 102512. https://doi.org/10.1016/j.xpro.2023.102512.