Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes
Тип публикации: Journal Article
Дата публикации: 2019-09-23
scimago Q4
wos Q4
БС3
SJR: 0.417
CiteScore: 2.6
Impact factor: 1.4
ISSN: 09671994, 14698730
PubMed ID:
31544726
Cell Biology
Developmental Biology
Краткое описание
Summary
Reference gene selection in mouse oocytes is an important task required to perform further adequate analysis of target gene expression levels. In the current work we have analyzed expression stability of the seven most commonly used reference genes (Actb, Eef1e1, Gapdh, H2afz, Ppia, Rpl4 and Ubc) in mouse oocytes at the germinal vesicle (GV) stage. We have performed analysis of expression stability of the above-mentioned reference genes with the three most commonly used software tools: geNorm, BestKeeper and NormFinder. Taking into account the results obtained from all of these programmes Gapdh, Rpl4 and H2afz seem to be suitable candidate reference genes in GV oocytes of mouse.
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ГОСТ
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Filatov M. A. et al. Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes // Zygote. 2019. Vol. 27. No. 6. pp. 392-397.
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Filatov M. A., Nikishin D. A., Khramova Y. V., Semenova M. L. Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes // Zygote. 2019. Vol. 27. No. 6. pp. 392-397.
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TY - JOUR
DO - 10.1017/S0967199419000492
UR - https://doi.org/10.1017/S0967199419000492
TI - Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes
T2 - Zygote
AU - Filatov, Maxim A
AU - Nikishin, D A
AU - Khramova, Y V
AU - Semenova, M L
PY - 2019
DA - 2019/09/23
PB - Cambridge University Press
SP - 392-397
IS - 6
VL - 27
PMID - 31544726
SN - 0967-1994
SN - 1469-8730
ER -
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@article{2019_Filatov,
author = {Maxim A Filatov and D A Nikishin and Y V Khramova and M L Semenova},
title = {Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes},
journal = {Zygote},
year = {2019},
volume = {27},
publisher = {Cambridge University Press},
month = {sep},
url = {https://doi.org/10.1017/S0967199419000492},
number = {6},
pages = {392--397},
doi = {10.1017/S0967199419000492}
}
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MLA
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Filatov, Maxim A., et al. “Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes.” Zygote, vol. 27, no. 6, Sep. 2019, pp. 392-397. https://doi.org/10.1017/S0967199419000492.
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