Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes
Publication type: Journal Article
Publication date: 2019-09-23
scimago Q4
wos Q4
SJR: 0.417
CiteScore: 2.6
Impact factor: 1.4
ISSN: 09671994, 14698730
PubMed ID:
31544726
Cell Biology
Developmental Biology
Abstract
Summary
Reference gene selection in mouse oocytes is an important task required to perform further adequate analysis of target gene expression levels. In the current work we have analyzed expression stability of the seven most commonly used reference genes (Actb, Eef1e1, Gapdh, H2afz, Ppia, Rpl4 and Ubc) in mouse oocytes at the germinal vesicle (GV) stage. We have performed analysis of expression stability of the above-mentioned reference genes with the three most commonly used software tools: geNorm, BestKeeper and NormFinder. Taking into account the results obtained from all of these programmes Gapdh, Rpl4 and H2afz seem to be suitable candidate reference genes in GV oocytes of mouse.
Found
Nothing found, try to update filter.
Found
Nothing found, try to update filter.
Top-30
Journals
|
1
2
|
|
|
Reproduction, Fertility and Development
2 publications, 28.57%
|
|
|
Fishes
1 publication, 14.29%
|
|
|
Journal of Assisted Reproduction and Genetics
1 publication, 14.29%
|
|
|
Biology of Reproduction
1 publication, 14.29%
|
|
|
Pharmaceuticals
1 publication, 14.29%
|
|
|
1
2
|
Publishers
|
1
2
|
|
|
MDPI
2 publications, 28.57%
|
|
|
CSIRO Publishing
2 publications, 28.57%
|
|
|
Springer Nature
1 publication, 14.29%
|
|
|
Society for the Study of Reproduction
1 publication, 14.29%
|
|
|
1
2
|
- We do not take into account publications without a DOI.
- Statistics recalculated weekly.
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
7
Total citations:
7
Citations from 2024:
1
(14.29%)
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Filatov M. A. et al. Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes // Zygote. 2019. Vol. 27. No. 6. pp. 392-397.
GOST all authors (up to 50)
Copy
Filatov M. A., Nikishin D. A., Khramova Y. V., Semenova M. L. Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes // Zygote. 2019. Vol. 27. No. 6. pp. 392-397.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1017/S0967199419000492
UR - https://doi.org/10.1017/S0967199419000492
TI - Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes
T2 - Zygote
AU - Filatov, Maxim A
AU - Nikishin, D A
AU - Khramova, Y V
AU - Semenova, M L
PY - 2019
DA - 2019/09/23
PB - Cambridge University Press
SP - 392-397
IS - 6
VL - 27
PMID - 31544726
SN - 0967-1994
SN - 1469-8730
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2019_Filatov,
author = {Maxim A Filatov and D A Nikishin and Y V Khramova and M L Semenova},
title = {Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes},
journal = {Zygote},
year = {2019},
volume = {27},
publisher = {Cambridge University Press},
month = {sep},
url = {https://doi.org/10.1017/S0967199419000492},
number = {6},
pages = {392--397},
doi = {10.1017/S0967199419000492}
}
Cite this
MLA
Copy
Filatov, Maxim A., et al. “Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes.” Zygote, vol. 27, no. 6, Sep. 2019, pp. 392-397. https://doi.org/10.1017/S0967199419000492.