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Lab team

Classical neurotransmitters such as serotonin, catecholamines and acetylcholine have functional activity already at the earliest stages of ontogenesis, even before the formation of the first nerve cells. They perform a wide range of functions, regulating and modulating various processes. Thus, biogenic amines are involved in the induction of egg maturation, the control of the cell cycle during cleavage divisions, the regulation of the activity of ciliary movements and the establishment of left-right asymmetry. These are just some of the described embryonic functions that these substances perform. Interestingly, the neural activity of neurotransmitters has been described in the embryonic development of representatives of various groups of the animal kingdom, including sponges and protozoa that do not have a nervous system. This indicates the widespread and evolutionary importance of neurotransmitters in embryonic development. The Embryophysiology Group is investigating the mechanisms by which neurotransmitters regulate the early stages of embryonic development and oogenesis. The team studies the interaction of neurotransmitters with other signaling pathways and factors to determine how these substances coordinate and tune the processes of embryogenesis. The results of these studies help us better understand the activity of neurotransmitters in the early stages of development and assess their importance for the normal development of the body.

  1. Real-time PCR (qPCR)
  2. Western blot
  3. Cloning
  4. Various methods of molecular biology
  5. Cell and tissue culture
  6. Confocal microscopy
  7. Experimental research methods
  8. Fluorescence microscopy
  9. PCR
  10. Real-time PCR and digital PCR, including design and validation of primers and probes to your target
  11. Immunocytochemistry
  12. Enzyme-linked immunosorbent assay (ELISA)
  13. High-efficiency liquid chromatography (HPLC)
  14. Reproductive toxicity (including embryonic and gonadotoxicity).
  15. Histochemistry
  16. Electron microscopy
  17. Bacterial cultures
  18. Immunohistochemistry (IHC)
  19. Spectrofluorometry
  20. Flow cytometry
Denis Nikishin 🥼 🤝
Head of Laboratory
Nina Alyoshina 🥼
Researcher
Nikishina, Yuliya Olegovna
Yuliya Nikishina 🤝
Researcher
Lyudmila Malychenko
Research Engineer
Maria Tkachenko
Senior Assistant
Dubrovskaya, Anna Sergeevna
Anna Dubrovskaya
Senior Assistant

Research directions

Structural and functional organization of transmitter signaling systems in early embryo cells

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Structural and functional organization of transmitter signaling systems in early embryo cells
It has been established that the mechanisms of functioning of transmitter systems in embryonic cells are based on the same key components as in synaptic transmission. However, they have a number of features. In one embryonic cell, several transmitters and several receptor mechanisms are often functionally active at once. During cell division, the signaling function of neurotransmitters, including binding to receptors or their analogues, is realized, at least in some cases, at the intracellular level. The group conducts a comprehensive study of the expression of receptors, transporters and other components of transmitter systems in early embryos of mammals, sea urchins and spur frogs. We also study their intracellular distribution, including co-localization with intracellular structures, including simultaneous expression and functional activity of several receptor proteins activated by the same transmitter in embryonic cells. * The figure shows the immunoreactivity against serotonin (red) in developing embryos and larvae of the polychaete worm Ophelia limacina. Serotonin is detected in the cytoplasm of oocytes and early embryos during the crushing period, but practically disappears by the gastrula stage. In the trochophore, serotonin is detected mainly in nerve cells and in the area of the ciliary cord.

The role of serotonin in the regulation of mammalian oogenesis and ovarian folliculogenesis

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The role of serotonin in the regulation of mammalian oogenesis and ovarian folliculogenesis
Serotonin is a factor in the maturation of eggs in many animals, including a stimulating effect on the processes of folliculogenesis in the mammalian ovary. It is shown that the functional activity of monoaminergic transmitters begins during the period of oogenesis, when not only the maturation of the egg occurs, but also the material basis for future embryonic development is laid. The main mechanism determining the functional activity of serotonin in the mammalian ovary is its capture by oocytes of growing ovarian follicles. The group is investigating the serotonergic regulation of folliculogenesis, including the mechanisms of side effects of antidepressants, primarily selective serotonin reuptake inhibitors (SSRIs), on female reproductive function. * In the figure, the ovary of a prepubescent mouse during the first wave of folliculogenesis (10-17 dpp) is a convenient experimental model that allows us to study the state of the ovarian pool and gonadotropin-independent period of early folliculogenesis.

Mechanisms of action of transmitters and their functional analogues in early embryonic development

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Mechanisms of action of transmitters and their functional analogues in early embryonic development
Research in this direction is conducted on sea urchin embryos using the classical model of blockade of the first cleavage division of the sea urchin. This allows for the quantification of the effects of embryotoxic substances. The cytostatic effect of dopamine and serotonin receptor antagonists at the intracellular level is mediated by their impact on cytoskeletal elements. These substances result in an increase in the degree of polymerization of the actin cytoskeleton and a disruption in the spatial organization of the division spindle. The figure illustrates the consequences of the blockade of the first division of the crushing of embryos of the sea urchin Paracentrotus lividus by the dopamine receptor antagonist haloperidol (left) and serotonin receptors ciproheptadine (right). The center depicts a control two-celled embryo that has completed the first cleavage division.

Publications and patents

Lab address

г. Москва, ул. Вавилова, 26, стр. 1
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