Biochemical and Structural Insights of the N-Methyltransferase CyaF in Cyanogramide Biosynthesis
Ruijie Chen
1, 2, 3
,
Qingbo Zhang
1, 2, 3, 4, 5, 6, 7, 8
,
Liping Zhang
1, 2, 3, 4, 5, 6, 7, 8
,
Chunyan Fang
1, 2
,
Hanning Zhu
1, 2, 4, 5
,
Weiming Zhu
9, 10, 11, 12
,
Changsheng Zhang
1, 2, 3, 4, 5, 6, 7, 8
,
Yiguang Zhu
1, 2, 3, 4, 5, 6, 7, 8
2
Chinese Academy of Sciences, Guangzhou, People’s Republic of China
|
4
State Key Laboratory of Tropical Oceanography, Key Laboratory of Tropical Marine Bioresources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology
|
5
Chinese Academy of sciences
7
Sanya Institute of Ocean Eco-Environmental Engineering
|
8
Sanya Institute of Ocean Eco-Environmental Engineering, Sanya, People’s Republic of China
|
9
Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy
11
Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Qingdao, People’s Republic of China
|
Publication type: Journal Article
Publication date: 2025-03-07
scimago Q1
wos Q1
SJR: 0.629
CiteScore: 6.5
Impact factor: 3.6
ISSN: 01633864, 15206025
Abstract
N-Methyltransferases involved in indole methylation have seldom been discovered in natural product biosynthesis. This study focuses on the enzyme CyaF, which catalyzes a critical N-methylation step of indole in the β-carboline skeleton during cyanogramide biosynthesis. Seven β-carboline analogues (3-9) were isolated from the recombinant strain Streptomyces coelicolor YF11/cyaABC, including three new compounds (5-7). In vitro assays revealed CyaF's substrate flexibility. The crystal structure of the CyaF/S-adenosyl-L-homocysteine (SAH) complex, combined with the AlphaFold-predicted model and site-directed mutagenesis, elucidated the catalytic mechanism and structural features that enable CyaF to accommodate diverse substrates, highlighting its potential for biocatalytic applications.
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Chen R. et al. Biochemical and Structural Insights of the N-Methyltransferase CyaF in Cyanogramide Biosynthesis // Journal of Natural Products. 2025. Vol. 88. No. 3. pp. 715-722.
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Chen R., Zhang Q., Zhang L., Fang C., Zhu H., Zhu W., Zhang C., Zhu Y. Biochemical and Structural Insights of the N-Methyltransferase CyaF in Cyanogramide Biosynthesis // Journal of Natural Products. 2025. Vol. 88. No. 3. pp. 715-722.
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TY - JOUR
DO - 10.1021/acs.jnatprod.4c01391
UR - https://pubs.acs.org/doi/10.1021/acs.jnatprod.4c01391
TI - Biochemical and Structural Insights of the N-Methyltransferase CyaF in Cyanogramide Biosynthesis
T2 - Journal of Natural Products
AU - Chen, Ruijie
AU - Zhang, Qingbo
AU - Zhang, Liping
AU - Fang, Chunyan
AU - Zhu, Hanning
AU - Zhu, Weiming
AU - Zhang, Changsheng
AU - Zhu, Yiguang
PY - 2025
DA - 2025/03/07
PB - American Chemical Society (ACS)
SP - 715-722
IS - 3
VL - 88
SN - 0163-3864
SN - 1520-6025
ER -
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@article{2025_Chen,
author = {Ruijie Chen and Qingbo Zhang and Liping Zhang and Chunyan Fang and Hanning Zhu and Weiming Zhu and Changsheng Zhang and Yiguang Zhu},
title = {Biochemical and Structural Insights of the N-Methyltransferase CyaF in Cyanogramide Biosynthesis},
journal = {Journal of Natural Products},
year = {2025},
volume = {88},
publisher = {American Chemical Society (ACS)},
month = {mar},
url = {https://pubs.acs.org/doi/10.1021/acs.jnatprod.4c01391},
number = {3},
pages = {715--722},
doi = {10.1021/acs.jnatprod.4c01391}
}
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Chen, Ruijie, et al. “Biochemical and Structural Insights of the N-Methyltransferase CyaF in Cyanogramide Biosynthesis.” Journal of Natural Products, vol. 88, no. 3, Mar. 2025, pp. 715-722. https://pubs.acs.org/doi/10.1021/acs.jnatprod.4c01391.