Journal of the American Chemical Society, volume 147, issue 13, pages 10903-10915

Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets

Matthew Penner ¹

Oskar James Klein ^{1, 2}

Maximilian Gantz ¹

Friederike E H Nintzel ¹

Anne-Cathrin Prowald ¹

Sally Boss ²

Paul K. Barker ²

P. Dupree ¹

Florian Hollfelder ¹

Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K. |

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K. |

Publication type: Journal Article

Publication date: 2025-03-24

American Chemical Society (ACS)

Journal: Journal of the American Chemical Society

scimago Q1

SJR: 5.489

CiteScore: 24.4

Impact factor: 14.4

ISSN: 00027863, 15205126

DOI: 10.1021/jacs.4c11804

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Publisher

American Chemical Society (ACS)

Journal

scimago Q1

SJR

5.489

CiteScore

24.4

Impact factor

14.4

ISSN

00027863 (Print)

15205126 (Electronic)