volume 19 issue 7 pages 2812-2824

Functional DNA Delivery Enabled by Lipid-Modified Charge-Altering Releasable Transporters (CARTs)

Nancy L Benner
Katherine E Near
Michael H. Bachmann
Christopher H. Contag
Publication typeJournal Article
Publication date2018-05-04
scimago Q1
wos Q1
SJR1.142
CiteScore9.2
Impact factor5.4
ISSN15257797, 15264602
Materials Chemistry
Polymers and Plastics
Bioengineering
Biomaterials
Abstract
Safe and effective DNA delivery systems are required to enable or enhance clinical strategies and research involving gene therapy and DNA vaccinations. To address this delivery problem, a series of charge-altering releasable transporters (CARTs) with varied lipid content were prepared and evaluated for plasmid DNA (pDNA) delivery into cultured cells. These lipid-modified CART co-oligomers were synthesized in only two steps via sequential organocatalytic ring-opening polymerization of lipid-containing cyclic carbonate monomers and morpholinone monomers. Lipid variations of the CARTs substantially impacted the delivery efficiency of pDNA, with oleyl- and linoleyl-based CARTs showing enhanced performance relative to the commercial transfection agent Lipofectamine 2000 (L2000). The best-performing oleyl CART was carried forward to study stable luciferase transfection with a Sleeping Beauty ( SB) transposon system. The oleyl CART outperformed the L2000 positive control with respect to stable transfection efficiency. CART-pDNA complexes represent a new DNA delivery system for research and clinical applications.
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GOST Copy
Benner N. L. et al. Functional DNA Delivery Enabled by Lipid-Modified Charge-Altering Releasable Transporters (CARTs) // Biomacromolecules. 2018. Vol. 19. No. 7. pp. 2812-2824.
GOST all authors (up to 50) Copy
Benner N. L., Near K. E., Bachmann M. H., Contag C. H., Waymouth R. M., WENDER P. H. Functional DNA Delivery Enabled by Lipid-Modified Charge-Altering Releasable Transporters (CARTs) // Biomacromolecules. 2018. Vol. 19. No. 7. pp. 2812-2824.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1021/acs.biomac.8b00401
UR - https://doi.org/10.1021/acs.biomac.8b00401
TI - Functional DNA Delivery Enabled by Lipid-Modified Charge-Altering Releasable Transporters (CARTs)
T2 - Biomacromolecules
AU - Benner, Nancy L
AU - Near, Katherine E
AU - Bachmann, Michael H.
AU - Contag, Christopher H.
AU - Waymouth, Robert M.
AU - WENDER, PAUL H.
PY - 2018
DA - 2018/05/04
PB - American Chemical Society (ACS)
SP - 2812-2824
IS - 7
VL - 19
PMID - 29727572
SN - 1525-7797
SN - 1526-4602
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2018_Benner,
author = {Nancy L Benner and Katherine E Near and Michael H. Bachmann and Christopher H. Contag and Robert M. Waymouth and PAUL H. WENDER},
title = {Functional DNA Delivery Enabled by Lipid-Modified Charge-Altering Releasable Transporters (CARTs)},
journal = {Biomacromolecules},
year = {2018},
volume = {19},
publisher = {American Chemical Society (ACS)},
month = {may},
url = {https://doi.org/10.1021/acs.biomac.8b00401},
number = {7},
pages = {2812--2824},
doi = {10.1021/acs.biomac.8b00401}
}
MLA
Cite this
MLA Copy
Benner, Nancy L., et al. “Functional DNA Delivery Enabled by Lipid-Modified Charge-Altering Releasable Transporters (CARTs).” Biomacromolecules, vol. 19, no. 7, May. 2018, pp. 2812-2824. https://doi.org/10.1021/acs.biomac.8b00401.