Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining
Maria V Babak
1
,
Pauline Le Faouder
1
,
Xavier Trivelli
2
,
Giorgia Pastorin
3
,
Gopalakrishnan Venkatesan
3
,
Mohammed Kajjout
1
,
Artem L Gushchin
5, 6
,
Muhammad Hanif
7
,
Olivier Poizat
8
,
Hervé Vezin
8
,
2
7
Publication type: Journal Article
Publication date: 2020-03-17
scimago Q1
wos Q1
SJR: 0.958
CiteScore: 7.4
Impact factor: 4.7
ISSN: 00201669, 1520510X
PubMed ID:
32181663
Inorganic Chemistry
Physical and Theoretical Chemistry
Abstract
The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).
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Total citations:
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Citations from 2024:
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(10%)
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GOST
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Babak M. V. et al. Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining // Inorganic Chemistry. 2020. Vol. 59. No. 7. pp. 4527-4535.
GOST all authors (up to 50)
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Babak M. V., Le Faouder P., Trivelli X., Pastorin G., Venkatesan G., Bezzubov S. I., Kajjout M., Gushchin A. L., Hanif M., Poizat O., Vezin H., Rolando C. Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining // Inorganic Chemistry. 2020. Vol. 59. No. 7. pp. 4527-4535.
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RIS
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TY - JOUR
DO - 10.1021/acs.inorgchem.9b03679
UR - https://pubs.acs.org/doi/10.1021/acs.inorgchem.9b03679
TI - Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining
T2 - Inorganic Chemistry
AU - Babak, Maria V
AU - Le Faouder, Pauline
AU - Trivelli, Xavier
AU - Pastorin, Giorgia
AU - Venkatesan, Gopalakrishnan
AU - Bezzubov, Stanislav I.
AU - Kajjout, Mohammed
AU - Gushchin, Artem L
AU - Hanif, Muhammad
AU - Poizat, Olivier
AU - Vezin, Hervé
AU - Rolando, Christian
PY - 2020
DA - 2020/03/17
PB - American Chemical Society (ACS)
SP - 4527-4535
IS - 7
VL - 59
PMID - 32181663
SN - 0020-1669
SN - 1520-510X
ER -
Cite this
BibTex (up to 50 authors)
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@article{2020_Babak,
author = {Maria V Babak and Pauline Le Faouder and Xavier Trivelli and Giorgia Pastorin and Gopalakrishnan Venkatesan and Stanislav I. Bezzubov and Mohammed Kajjout and Artem L Gushchin and Muhammad Hanif and Olivier Poizat and Hervé Vezin and Christian Rolando},
title = {Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining},
journal = {Inorganic Chemistry},
year = {2020},
volume = {59},
publisher = {American Chemical Society (ACS)},
month = {mar},
url = {https://pubs.acs.org/doi/10.1021/acs.inorgchem.9b03679},
number = {7},
pages = {4527--4535},
doi = {10.1021/acs.inorgchem.9b03679}
}
Cite this
MLA
Copy
Babak, Maria V., et al. “Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining.” Inorganic Chemistry, vol. 59, no. 7, Mar. 2020, pp. 4527-4535. https://pubs.acs.org/doi/10.1021/acs.inorgchem.9b03679.