Inorganic Chemistry

, том 59 , издание 7 , страницы 4527-4535

Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

Тип публикации: Journal Article

Дата публикации: 2020-03-17

American Chemical Society (ACS)

Inorganic Chemistry

scimago Q1

wos Q1

white level БС1

SJR: 0.958

CiteScore: 7.4

Impact factor: 4.7

ISSN: 00201669, 1520510X

DOI: 10.1021/acs.inorgchem.9b03679

Скопировать DOI

PubMed ID: 32181663

Inorganic Chemistry

Physical and Theoretical Chemistry

Краткое описание

The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).

Для доступа к списку цитирований публикации необходимо авторизоваться.

Войти с ORCID

Для доступа к списку профилей, цитирующих публикацию, необходимо авторизоваться.

Войти с ORCID

Топ-30

Журналы

	1 2
Inorganic Chemistry	Inorganic Chemistry, 2, 20% Inorganic Chemistry 2 публикации, 20%
Dalton Transactions	Dalton Transactions, 1, 10% Dalton Transactions 1 публикация, 10%
Journal of the American Chemical Society	Journal of the American Chemical Society, 1, 10% Journal of the American Chemical Society 1 публикация, 10%
Frontiers in Chemistry	Frontiers in Chemistry, 1, 10% Frontiers in Chemistry 1 публикация, 10%
Chemical Engineering Science	Chemical Engineering Science, 1, 10% Chemical Engineering Science 1 публикация, 10%
Journal of Physics: Conference Series	Journal of Physics: Conference Series, 1, 10% Journal of Physics: Conference Series 1 публикация, 10%
Photochemistry and Photobiology	Photochemistry and Photobiology, 1, 10% Photochemistry and Photobiology 1 публикация, 10%
Annual Reports on NMR Spectroscopy	Annual Reports on NMR Spectroscopy, 1, 10% Annual Reports on NMR Spectroscopy 1 публикация, 10%
Microchemical Journal	Microchemical Journal, 1, 10% Microchemical Journal 1 публикация, 10%
	1 2

Издатели

	1 2 3
American Chemical Society (ACS)	American Chemical Society (ACS), 3, 30% American Chemical Society (ACS) 3 публикации, 30%
Elsevier	Elsevier, 3, 30% Elsevier 3 публикации, 30%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 10% Royal Society of Chemistry (RSC) 1 публикация, 10%
Frontiers Media S.A.	Frontiers Media S.A., 1, 10% Frontiers Media S.A. 1 публикация, 10%
IOP Publishing	IOP Publishing, 1, 10% IOP Publishing 1 публикация, 10%
Wiley	Wiley, 1, 10% Wiley 1 публикация, 10%
	1 2 3

Мы не учитываем публикации, у которых нет DOI.
Статистика публикаций обновляется еженедельно.

Вы ученый?

Создайте профиль, чтобы получать персональные рекомендации коллег, конференций и новых статей.

Войти с ORCID

Метрики

Цитировать

ГОСТ |

Цитировать

ГОСТ Скопировать

Babak M. V. et al. Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining // Inorganic Chemistry. 2020. Vol. 59. No. 7. pp. 4527-4535.

ГОСТ со всеми авторами (до 50) Скопировать

Babak M. V., Le Faouder P., Trivelli X., Pastorin G., Venkatesan G., Bezzubov S. I., Kajjout M., Gushchin A. L., Hanif M., Poizat O., Vezin H., Rolando C. Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining // Inorganic Chemistry. 2020. Vol. 59. No. 7. pp. 4527-4535.

RIS |

Цитировать

RIS Скопировать

TY - JOUR

DO - 10.1021/acs.inorgchem.9b03679

UR - https://pubs.acs.org/doi/10.1021/acs.inorgchem.9b03679

TI - Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

T2 - Inorganic Chemistry

AU - Babak, Maria V

AU - Le Faouder, Pauline

AU - Trivelli, Xavier

AU - Pastorin, Giorgia

AU - Venkatesan, Gopalakrishnan

AU - Bezzubov, Stanislav I

AU - Kajjout, Mohammed

AU - Gushchin, Artem L

AU - Hanif, Muhammad

AU - Poizat, Olivier

AU - Vezin, Hervé

AU - Rolando, Christian

PY - 2020

DA - 2020/03/17

PB - American Chemical Society (ACS)

SP - 4527-4535

IS - 7

VL - 59

PMID - 32181663

SN - 0020-1669

SN - 1520-510X

ER -

BibTex |

Цитировать

BibTex (до 50 авторов) Скопировать

@article{2020_Babak,

author = {Maria V Babak and Pauline Le Faouder and Xavier Trivelli and Giorgia Pastorin and Gopalakrishnan Venkatesan and Stanislav I Bezzubov and Mohammed Kajjout and Artem L Gushchin and Muhammad Hanif and Olivier Poizat and Hervé Vezin and Christian Rolando},

title = {Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining},

journal = {Inorganic Chemistry},

year = {2020},

volume = {59},

publisher = {American Chemical Society (ACS)},

month = {mar},

url = {https://pubs.acs.org/doi/10.1021/acs.inorgchem.9b03679},

number = {7},

pages = {4527--4535},

doi = {10.1021/acs.inorgchem.9b03679}

}

MLA

Цитировать

MLA Скопировать

Babak, Maria V., et al. “Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining.” Inorganic Chemistry, vol. 59, no. 7, Mar. 2020, pp. 4527-4535. https://pubs.acs.org/doi/10.1021/acs.inorgchem.9b03679.

Издатель

American Chemical Society (ACS)

Журнал

Inorganic Chemistry

scimago Q1

wos Q1

white level БС1