Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor
Giwan Seo
1, 2
,
Geonhee Lee
3
,
Mi-Jeong Kim
1, 2
,
Seung-Hwa Baek
2, 4
,
Minsuk Choi
2
,
Keun Bon Ku
2
,
Chang-Seop Lee
5, 6
,
Sangmi Jun
2, 7
,
Daeui Park
2, 4
,
Hong Gi Kim
2
,
Seong-Jun Kim
2
,
Jeong-O Lee
3
,
Bum-Tae Kim
2
,
Edmond Changkyun Park
1, 2, 8
,
Seung Il Kim
1, 2, 8
4
Publication type: Journal Article
Publication date: 2020-04-15
scimago Q1
wos Q1
SJR: 4.497
CiteScore: 24.2
Impact factor: 16.0
ISSN: 19360851, 1936086X
PubMed ID:
32293168
General Physics and Astronomy
General Materials Science
General Engineering
Abstract
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.
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Seo G. et al. Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor // ACS Nano. 2020. Vol. 14. No. 4. pp. 5135-5142.
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Seo G., Lee G., Kim M., Baek S., Choi M., Ku K. B., Lee C., Jun S., Park D., Kim H. G., Kim S., Lee J., Kim B., Park E. C., Kim S. I. Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor // ACS Nano. 2020. Vol. 14. No. 4. pp. 5135-5142.
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TY - JOUR
DO - 10.1021/acsnano.0c02823
UR - https://doi.org/10.1021/acsnano.0c02823
TI - Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor
T2 - ACS Nano
AU - Seo, Giwan
AU - Lee, Geonhee
AU - Kim, Mi-Jeong
AU - Baek, Seung-Hwa
AU - Choi, Minsuk
AU - Ku, Keun Bon
AU - Lee, Chang-Seop
AU - Jun, Sangmi
AU - Park, Daeui
AU - Kim, Hong Gi
AU - Kim, Seong-Jun
AU - Lee, Jeong-O
AU - Kim, Bum-Tae
AU - Park, Edmond Changkyun
AU - Kim, Seung Il
PY - 2020
DA - 2020/04/15
PB - American Chemical Society (ACS)
SP - 5135-5142
IS - 4
VL - 14
PMID - 32293168
SN - 1936-0851
SN - 1936-086X
ER -
Cite this
BibTex (up to 50 authors)
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@article{2020_Seo,
author = {Giwan Seo and Geonhee Lee and Mi-Jeong Kim and Seung-Hwa Baek and Minsuk Choi and Keun Bon Ku and Chang-Seop Lee and Sangmi Jun and Daeui Park and Hong Gi Kim and Seong-Jun Kim and Jeong-O Lee and Bum-Tae Kim and Edmond Changkyun Park and Seung Il Kim},
title = {Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor},
journal = {ACS Nano},
year = {2020},
volume = {14},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1021/acsnano.0c02823},
number = {4},
pages = {5135--5142},
doi = {10.1021/acsnano.0c02823}
}
Cite this
MLA
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Seo, Giwan, et al. “Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.” ACS Nano, vol. 14, no. 4, Apr. 2020, pp. 5135-5142. https://doi.org/10.1021/acsnano.0c02823.