ACS Chemical Biology, volume 5, issue 9, pages 875-885
A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells.
1
DEPARTMENT OF CHEMISTRY
2
Department of Pharmaceutical Sciences
Publication type: Journal Article
Publication date: 2010-07-28
Journal:
ACS Chemical Biology
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 4
ISSN: 15548929, 15548937
PubMed ID:
20666508
Biochemistry
General Medicine
Molecular Medicine
Abstract
The nonsymmetrical spatial distribution of newly synthesized proteins in animal cells plays a central role in many cellular processes. Here, we report that a simple alkene tag, homoallylglycine (HAG), was co-translationally incorporated into a recombinant protein as well as endogenous, newly synthesized proteins in mammalian cells with high efficiency. In conjunction with a photoinduced tetrazole-alkene cycloaddition reaction ("photoclick chemistry"), this alkene tag further served as a bioorthogonal chemical reporter both for the selective protein functionalization in vitro and for a spatiotemporally controlled imaging of the newly synthesized proteins in live mammalian cells. This two-step metabolic alkene tagging-photocontrolled chemical functionalization approach may offer a potentially useful tool to study the role of spatiotemporally regulated protein synthesis in mammalian cells.
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Citations by publishers
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5
10
15
20
25
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American Chemical Society (ACS)
22 publications, 30.14%
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Royal Society of Chemistry (RSC)
20 publications, 27.4%
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Wiley
11 publications, 15.07%
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Springer Nature
6 publications, 8.22%
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Elsevier
6 publications, 8.22%
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 1.37%
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IOP Publishing
1 publication, 1.37%
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Public Library of Science (PLoS)
1 publication, 1.37%
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American Society for Biochemistry and Molecular Biology
1 publication, 1.37%
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Pleiades Publishing
1 publication, 1.37%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 1.37%
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5
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25
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Song W. et al. A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells. // ACS Chemical Biology. 2010. Vol. 5. No. 9. pp. 875-885.
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Song W., Wang Y., Yu Z., Vera C. I. R., Qu J., Lin Q. A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells. // ACS Chemical Biology. 2010. Vol. 5. No. 9. pp. 875-885.
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TY - JOUR
DO - 10.1021/cb100193h
UR - https://doi.org/10.1021/cb100193h
TI - A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells.
T2 - ACS Chemical Biology
AU - Song, Wenjiao
AU - Wang, Yizhong
AU - Vera, Claudia I Rivera
AU - Yu, Zhipeng
AU - Qu, Jun
AU - Lin, Qing
PY - 2010
DA - 2010/07/28 00:00:00
PB - American Chemical Society (ACS)
SP - 875-885
IS - 9
VL - 5
PMID - 20666508
SN - 1554-8929
SN - 1554-8937
ER -
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@article{2010_Song,
author = {Wenjiao Song and Yizhong Wang and Claudia I Rivera Vera and Zhipeng Yu and Jun Qu and Qing Lin},
title = {A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells.},
journal = {ACS Chemical Biology},
year = {2010},
volume = {5},
publisher = {American Chemical Society (ACS)},
month = {jul},
url = {https://doi.org/10.1021/cb100193h},
number = {9},
pages = {875--885},
doi = {10.1021/cb100193h}
}
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MLA
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Song, Wenjiao, et al. “A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells..” ACS Chemical Biology, vol. 5, no. 9, Jul. 2010, pp. 875-885. https://doi.org/10.1021/cb100193h.