ACS Chemical Biology, volume 5, issue 9, pages 875-885
A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells.
1
DEPARTMENT OF CHEMISTRY
2
Department of Pharmaceutical Sciences
Publication type: Journal Article
Publication date: 2010-07-28
Journal:
ACS Chemical Biology
Quartile SCImago
Q1
Quartile WOS
Q2
SJR: 1.344
CiteScore: 7.5
Impact factor: 3.5
ISSN: 15548929, 15548937
PubMed ID:
20666508
Biochemistry
General Medicine
Molecular Medicine
Abstract
The nonsymmetrical spatial distribution of newly synthesized proteins in animal cells plays a central role in many cellular processes. Here, we report that a simple alkene tag, homoallylglycine (HAG), was co-translationally incorporated into a recombinant protein as well as endogenous, newly synthesized proteins in mammalian cells with high efficiency. In conjunction with a photoinduced tetrazole-alkene cycloaddition reaction ("photoclick chemistry"), this alkene tag further served as a bioorthogonal chemical reporter both for the selective protein functionalization in vitro and for a spatiotemporally controlled imaging of the newly synthesized proteins in live mammalian cells. This two-step metabolic alkene tagging-photocontrolled chemical functionalization approach may offer a potentially useful tool to study the role of spatiotemporally regulated protein synthesis in mammalian cells.
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GOST
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Song W. et al. A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells. // ACS Chemical Biology. 2010. Vol. 5. No. 9. pp. 875-885.
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Song W., Wang Y., Yu Z., Vera C. I. R., Qu J., Lin Q. A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells. // ACS Chemical Biology. 2010. Vol. 5. No. 9. pp. 875-885.
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RIS
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TY - JOUR
DO - 10.1021/cb100193h
UR - https://doi.org/10.1021/cb100193h
TI - A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells.
T2 - ACS Chemical Biology
AU - Song, Wenjiao
AU - Wang, Yizhong
AU - Vera, Claudia I Rivera
AU - Yu, Zhipeng
AU - Qu, Jun
AU - Lin, Qing
PY - 2010
DA - 2010/07/28
PB - American Chemical Society (ACS)
SP - 875-885
IS - 9
VL - 5
PMID - 20666508
SN - 1554-8929
SN - 1554-8937
ER -
Cite this
BibTex
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@article{2010_Song,
author = {Wenjiao Song and Yizhong Wang and Claudia I Rivera Vera and Zhipeng Yu and Jun Qu and Qing Lin},
title = {A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells.},
journal = {ACS Chemical Biology},
year = {2010},
volume = {5},
publisher = {American Chemical Society (ACS)},
month = {jul},
url = {https://doi.org/10.1021/cb100193h},
number = {9},
pages = {875--885},
doi = {10.1021/cb100193h}
}
Cite this
MLA
Copy
Song, Wenjiao, et al. “A metabolic alkene reporter for spatiotemporally controlled imaging of newly synthesized proteins in Mammalian cells..” ACS Chemical Biology, vol. 5, no. 9, Jul. 2010, pp. 875-885. https://doi.org/10.1021/cb100193h.