Intramolecular electron-transfer rates in ferrocene-derivatized glucose oxidase
Тип публикации: Journal Article
Дата публикации: 1993-08-01
scimago Q1
wos Q1
БС1
SJR: 5.489
CiteScore: 24.4
Impact factor: 15.6
ISSN: 00027863, 15205126
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Краткое описание
Glucose oxidase (GOx) was covalently modified at pH 7.2 with ferrocenecarboxylic acid (FCA), ferrocenedicarboxylic acid (FDA), and ferroceneacetic acid (FAA) using 1-ethyl-3-(3-(dimethylamino)propyl) - carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide to promote selective coupling to surface lysines. Reagent ratios were varied to obtain derivatives with 5-12 ferrocene groups per GOx dimer and ≥60% activity. For comparison, GOx was derivatized with FCA in the presence of 3 M urea using only EDC as a promoter. Varying reagent ratios yielded derivatives with 439 FCA groups per GOx and ≤30% activity; linear sweep voltammetry results showed a slow but readily detectable release of FCA upon storage of these derivatives
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120
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ГОСТ |
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BibTex |
MLA
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ГОСТ
Скопировать
Badia A. et al. Intramolecular electron-transfer rates in ferrocene-derivatized glucose oxidase // Journal of the American Chemical Society. 1993. Vol. 115. No. 16. pp. 7053-7060.
ГОСТ со всеми авторами (до 50)
Скопировать
Badia A., Carlini R., Fernandez A., Battaglini F., Mikkelsen S. R., English A. M. Intramolecular electron-transfer rates in ferrocene-derivatized glucose oxidase // Journal of the American Chemical Society. 1993. Vol. 115. No. 16. pp. 7053-7060.
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RIS
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TY - JOUR
DO - 10.1021/ja00069a001
UR - https://doi.org/10.1021/ja00069a001
TI - Intramolecular electron-transfer rates in ferrocene-derivatized glucose oxidase
T2 - Journal of the American Chemical Society
AU - Badia, Antonella
AU - Carlini, Rina
AU - Fernandez, Arthur
AU - Battaglini, F.
AU - Mikkelsen, Susan R.
AU - English, Ann M.
PY - 1993
DA - 1993/08/01
PB - American Chemical Society (ACS)
SP - 7053-7060
IS - 16
VL - 115
SN - 0002-7863
SN - 1520-5126
ER -
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BibTex (до 50 авторов)
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@article{1993_Badia,
author = {Antonella Badia and Rina Carlini and Arthur Fernandez and F. Battaglini and Susan R. Mikkelsen and Ann M. English},
title = {Intramolecular electron-transfer rates in ferrocene-derivatized glucose oxidase},
journal = {Journal of the American Chemical Society},
year = {1993},
volume = {115},
publisher = {American Chemical Society (ACS)},
month = {aug},
url = {https://doi.org/10.1021/ja00069a001},
number = {16},
pages = {7053--7060},
doi = {10.1021/ja00069a001}
}
Цитировать
MLA
Скопировать
Badia, Antonella, et al. “Intramolecular electron-transfer rates in ferrocene-derivatized glucose oxidase.” Journal of the American Chemical Society, vol. 115, no. 16, Aug. 1993, pp. 7053-7060. https://doi.org/10.1021/ja00069a001.