Journal of the American Chemical Society, volume 127, issue 21, pages 7932-7940
Sensitivity and Specificity of Metal Surface-Immobilized “Molecular Beacon” Biosensors
1
Contribution from the Department of Dermatology, Department of Biochemistry and Biophysics, Department of Chemistry, and The Center for Future Health, University of Rochester, Rochester, New York
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Publication type: Journal Article
Publication date: 2005-05-05
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 15
ISSN: 00027863, 15205126
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Abstract
The separate developments of microarray patterning of DNA oligonucleotides, and of DNA hairpins as sensitive probes for oligonucleotide identification in solution, have had a tremendous impact on basic biological research and clinical applications. We have combined these two approaches to develop arrayable and label-free biological sensors based on fluorescence unquenching of DNA hairpins immobilized on metal surfaces. The thermodynamic and kinetic response of these sensors, and the factors important in hybridization efficiency, were investigated. Hybridization efficiency was found to be sensitive to hairpin secondary structure, as well as to the surface distribution of DNA hairpins on the substrate. The identity of the bases used in the hairpin stem as well as the overall loop length significantly affected sensitivity and selectivity. Surface-immobilized hairpins discriminated between two sequences with a single base-pair mismatch with high sensitivity (over an order of magnitude difference in signal) under identical assay conditions (no change in stringency). This represents a significant improvement over other microarray-based techniques.
Top-30
Citations by journals
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Angewandte Chemie
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Angewandte Chemie - International Edition
5 publications, 2.67%
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4 publications, 2.14%
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4 publications, 2.14%
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3 publications, 1.6%
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3 publications, 1.6%
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Microchimica Acta
3 publications, 1.6%
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Talanta
3 publications, 1.6%
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Chemistry - A European Journal
3 publications, 1.6%
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Chemical Reviews
3 publications, 1.6%
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ACS Nano
3 publications, 1.6%
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Analytical Methods
3 publications, 1.6%
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2 publications, 1.07%
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Analytica Chimica Acta
2 publications, 1.07%
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Electrochimica Acta
2 publications, 1.07%
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ACS applied materials & interfaces
2 publications, 1.07%
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ACS Applied Bio Materials
2 publications, 1.07%
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Soft Matter
2 publications, 1.07%
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Nanoscale
2 publications, 1.07%
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Applied Physics Letters
1 publication, 0.53%
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Biomicrofluidics
1 publication, 0.53%
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Journal of Applied Physics
1 publication, 0.53%
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5
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25
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Citations by publishers
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5
10
15
20
25
30
35
40
45
50
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Elsevier
48 publications, 25.67%
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American Chemical Society (ACS)
47 publications, 25.13%
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Royal Society of Chemistry (RSC)
25 publications, 13.37%
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Wiley
22 publications, 11.76%
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Springer Nature
16 publications, 8.56%
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Multidisciplinary Digital Publishing Institute (MDPI)
7 publications, 3.74%
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American Institute of Physics (AIP)
3 publications, 1.6%
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Japan Society for Analytical Chemistry
2 publications, 1.07%
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Future Medicine
1 publication, 0.53%
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Institution of Engineering and Technology (IET)
1 publication, 0.53%
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SPIE
1 publication, 0.53%
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Future Science
1 publication, 0.53%
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China Science Publishing & Media
1 publication, 0.53%
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Taylor & Francis
1 publication, 0.53%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 0.53%
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5
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Du H. et al. Sensitivity and Specificity of Metal Surface-Immobilized “Molecular Beacon” Biosensors // Journal of the American Chemical Society. 2005. Vol. 127. No. 21. pp. 7932-7940.
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Du H., Strohsahl C. M., Camera J., Miller B. L., Krauss T. Sensitivity and Specificity of Metal Surface-Immobilized “Molecular Beacon” Biosensors // Journal of the American Chemical Society. 2005. Vol. 127. No. 21. pp. 7932-7940.
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TY - JOUR
DO - 10.1021/ja042482a
UR - https://doi.org/10.1021/ja042482a
TI - Sensitivity and Specificity of Metal Surface-Immobilized “Molecular Beacon” Biosensors
T2 - Journal of the American Chemical Society
AU - Strohsahl, Christopher M.
AU - Camera, James
AU - Miller, Benjamin L.
AU - Du, Hui
AU - Krauss, Todd
PY - 2005
DA - 2005/05/05 00:00:00
PB - American Chemical Society (ACS)
SP - 7932-7940
IS - 21
VL - 127
SN - 0002-7863
SN - 1520-5126
ER -
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@article{2005_Du,
author = {Christopher M. Strohsahl and James Camera and Benjamin L. Miller and Hui Du and Todd Krauss},
title = {Sensitivity and Specificity of Metal Surface-Immobilized “Molecular Beacon” Biosensors},
journal = {Journal of the American Chemical Society},
year = {2005},
volume = {127},
publisher = {American Chemical Society (ACS)},
month = {may},
url = {https://doi.org/10.1021/ja042482a},
number = {21},
pages = {7932--7940},
doi = {10.1021/ja042482a}
}
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MLA
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Du, Hui, et al. “Sensitivity and Specificity of Metal Surface-Immobilized “Molecular Beacon” Biosensors.” Journal of the American Chemical Society, vol. 127, no. 21, May. 2005, pp. 7932-7940. https://doi.org/10.1021/ja042482a.