Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase.
Тип публикации: Journal Article
Дата публикации: 2005-03-25
scimago Q1
wos Q1
БС1
SJR: 5.489
CiteScore: 24.4
Impact factor: 15.6
ISSN: 00027863, 15205126
PubMed ID:
15826149
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Краткое описание
This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex.
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Tremblay L. W. et al. Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase. // Journal of the American Chemical Society. 2005. Vol. 127. No. 15. pp. 5298-5299.
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Tremblay L. W., Zhang G., Dai J., Dunaway-Mariano D., Allen K. Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase. // Journal of the American Chemical Society. 2005. Vol. 127. No. 15. pp. 5298-5299.
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TY - JOUR
DO - 10.1021/ja0509073
UR - https://doi.org/10.1021/ja0509073
TI - Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase.
T2 - Journal of the American Chemical Society
AU - Tremblay, Lee W
AU - Zhang, Guofeng
AU - Dai, Jianying
AU - Dunaway-Mariano, Debra
AU - Allen, Karen
PY - 2005
DA - 2005/03/25
PB - American Chemical Society (ACS)
SP - 5298-5299
IS - 15
VL - 127
PMID - 15826149
SN - 0002-7863
SN - 1520-5126
ER -
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@article{2005_Tremblay,
author = {Lee W Tremblay and Guofeng Zhang and Jianying Dai and Debra Dunaway-Mariano and Karen Allen},
title = {Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase.},
journal = {Journal of the American Chemical Society},
year = {2005},
volume = {127},
publisher = {American Chemical Society (ACS)},
month = {mar},
url = {https://doi.org/10.1021/ja0509073},
number = {15},
pages = {5298--5299},
doi = {10.1021/ja0509073}
}
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MLA
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Tremblay, Lee W., et al. “Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase..” Journal of the American Chemical Society, vol. 127, no. 15, Mar. 2005, pp. 5298-5299. https://doi.org/10.1021/ja0509073.