Lipid modification of proteins through sortase-catalyzed transpeptidation.
Publication type: Journal Article
Publication date: 2008-11-07
scimago Q1
wos Q1
SJR: 5.554
CiteScore: 22.5
Impact factor: 15.6
ISSN: 00027863, 15205126
PubMed ID:
18989959
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Abstract
A general chemoenzymatic method for the site-specific attachment of lipids to protein substrates is described. Sortase A is used to append short lipid-modified oligoglycine peptides to the C terminus of protein substrates bearing a five amino acid sortase A recognition sequence (LPETG). We demonstrate the attachment of a range of hydrophobic modifications in excellent yield (60-90%), including a simple step for removing the sortase enzyme postreaction. Lipoproteins prepared using these procedures were subsequently shown to associate with mammalian cells in a lipid tail-dependent fashion and localized to the plasma membrane and endosomes.
Found
Nothing found, try to update filter.
Found
Nothing found, try to update filter.
Top-30
Journals
|
2
4
6
8
10
12
|
|
|
Angewandte Chemie
12 publications, 8.33%
|
|
|
Angewandte Chemie - International Edition
12 publications, 8.33%
|
|
|
Bioconjugate Chemistry
10 publications, 6.94%
|
|
|
ChemBioChem
7 publications, 4.86%
|
|
|
Journal of the American Chemical Society
7 publications, 4.86%
|
|
|
Chemistry - A European Journal
4 publications, 2.78%
|
|
|
Proceedings of the National Academy of Sciences of the United States of America
4 publications, 2.78%
|
|
|
Nature Protocols
3 publications, 2.08%
|
|
|
Journal of Biological Chemistry
3 publications, 2.08%
|
|
|
European Journal of Pharmaceutics and Biopharmaceutics
3 publications, 2.08%
|
|
|
Biotechnology Journal
3 publications, 2.08%
|
|
|
Biochemistry
3 publications, 2.08%
|
|
|
Chemical Society Reviews
3 publications, 2.08%
|
|
|
Molecules
2 publications, 1.39%
|
|
|
PLoS ONE
2 publications, 1.39%
|
|
|
Current Opinion in Chemical Biology
2 publications, 1.39%
|
|
|
Current Protocols in Protein Science
2 publications, 1.39%
|
|
|
Biotechnology and Bioengineering
2 publications, 1.39%
|
|
|
ACS Chemical Biology
2 publications, 1.39%
|
|
|
mAbs
2 publications, 1.39%
|
|
|
ACS Omega
2 publications, 1.39%
|
|
|
Bioorganic and Medicinal Chemistry Letters
1 publication, 0.69%
|
|
|
Bulletin of the Chemical Society of Japan
1 publication, 0.69%
|
|
|
Experimental Biology and Medicine
1 publication, 0.69%
|
|
|
Cancers
1 publication, 0.69%
|
|
|
Polymers
1 publication, 0.69%
|
|
|
Journal of Biomolecular NMR
1 publication, 0.69%
|
|
|
Scientific Reports
1 publication, 0.69%
|
|
|
Journal of Nanobiotechnology
1 publication, 0.69%
|
|
|
2
4
6
8
10
12
|
Publishers
|
10
20
30
40
50
|
|
|
Wiley
50 publications, 34.72%
|
|
|
American Chemical Society (ACS)
30 publications, 20.83%
|
|
|
Elsevier
14 publications, 9.72%
|
|
|
Springer Nature
11 publications, 7.64%
|
|
|
Royal Society of Chemistry (RSC)
10 publications, 6.94%
|
|
|
MDPI
5 publications, 3.47%
|
|
|
Proceedings of the National Academy of Sciences (PNAS)
4 publications, 2.78%
|
|
|
American Society for Biochemistry and Molecular Biology
3 publications, 2.08%
|
|
|
Taylor & Francis
3 publications, 2.08%
|
|
|
Cold Spring Harbor Laboratory
3 publications, 2.08%
|
|
|
Public Library of Science (PLoS)
2 publications, 1.39%
|
|
|
American Association for Cancer Research (AACR)
2 publications, 1.39%
|
|
|
Oxford University Press
1 publication, 0.69%
|
|
|
SAGE
1 publication, 0.69%
|
|
|
IOP Publishing
1 publication, 0.69%
|
|
|
Rockefeller University Press
1 publication, 0.69%
|
|
|
Annual Reviews
1 publication, 0.69%
|
|
|
OOO Zhurnal "Mendeleevskie Soobshcheniya"
1 publication, 0.69%
|
|
|
10
20
30
40
50
|
- We do not take into account publications without a DOI.
- Statistics recalculated weekly.
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
144
Total citations:
144
Citations from 2025:
5
(3.47%)
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Antos J. M. et al. Lipid modification of proteins through sortase-catalyzed transpeptidation. // Journal of the American Chemical Society. 2008. Vol. 130. No. 48. pp. 16338-16343.
GOST all authors (up to 50)
Copy
Antos J. M., Miller G. M., Grotenbreg G. M., Ploegh H. L. Lipid modification of proteins through sortase-catalyzed transpeptidation. // Journal of the American Chemical Society. 2008. Vol. 130. No. 48. pp. 16338-16343.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1021/ja806779e
UR - https://doi.org/10.1021/ja806779e
TI - Lipid modification of proteins through sortase-catalyzed transpeptidation.
T2 - Journal of the American Chemical Society
AU - Antos, John M
AU - Miller, Gwenn M
AU - Grotenbreg, Gijsbert M.
AU - Ploegh, Hidde L.
PY - 2008
DA - 2008/11/07
PB - American Chemical Society (ACS)
SP - 16338-16343
IS - 48
VL - 130
PMID - 18989959
SN - 0002-7863
SN - 1520-5126
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2008_Antos,
author = {John M Antos and Gwenn M Miller and Gijsbert M. Grotenbreg and Hidde L. Ploegh},
title = {Lipid modification of proteins through sortase-catalyzed transpeptidation.},
journal = {Journal of the American Chemical Society},
year = {2008},
volume = {130},
publisher = {American Chemical Society (ACS)},
month = {nov},
url = {https://doi.org/10.1021/ja806779e},
number = {48},
pages = {16338--16343},
doi = {10.1021/ja806779e}
}
Cite this
MLA
Copy
Antos, John M., et al. “Lipid modification of proteins through sortase-catalyzed transpeptidation..” Journal of the American Chemical Society, vol. 130, no. 48, Nov. 2008, pp. 16338-16343. https://doi.org/10.1021/ja806779e.