Journal of the American Chemical Society

, volume 118 , issue 28 , pages 6556-6561

Copper−Zinc Superoxide Dismutase: Why Not pH-Dependent?

L. M. Ellerby ¹

Diane E. Cabelli ¹

Janet A Graden ¹

J. S. Valentine ¹

Hide authors affiliations Show authors affiliations: 1 affiliation

Contribution from the Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095-1569, and Chemistry Department, Brookhaven National Laboratory, Upton, New York 11973

Brookhaven National Laboratory

University of California, Los Angeles

Publication type: Journal Article

Publication date: 1996-01-01

American Chemical Society (ACS)

Journal of the American Chemical Society

scimago Q1

wos Q1

SJR: 5.554

CiteScore: 22.5

Impact factor: 15.6

ISSN: 00027863, 15205126

DOI: 10.1021/ja953845x

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General Chemistry

Catalysis

Biochemistry

Colloid and Surface Chemistry

Abstract

Copper−zinc superoxide dismutase (CuZnSOD) catalyzes the disproportionation of superoxide to hydrogen peroxide and dioxygen at diffusion controlled rates. Previous mechanistic studies have focused on the dramatic electrostatic guidance mechanism by which superoxide is drawn into the active site of this enzyme. Another striking but less understood feature of this enzyme is its ability to dismutate superoxide over a wide range of pH (5−9.5) without any change in rate-determining step or structural changes at the active site copper. To investigate the explanation for this pH independence, we have redetermined the rate of superoxide disproportionation, kcat, catalyzed by the zinc-deficient form (Cu-apoSOD) of the enzyme as a function of pH and have found that it is pH-dependent, in contrast to the native enzyme, even under conditions in which the copper ion does not leave the native copper-binding site. In addition, we have determined the rate of reduction, k1, of Cu-apoSOD by superoxide and have found that t...

Found

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Churakov Andrei

PhD in Chemistry

555 publications, 7 054 citations, 14 reviews

h-index: 38

Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences

1 citation

Medvedev Alexander

PhD in Chemistry

82 publications, 2 183 citations

h-index: 24

Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences

Moscow Institute of Physics and Technology

1 citation

Mikhaylov Alexey

PhD in Chemistry

62 publications, 1 406 citations

h-index: 21

Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences

Research interests

Coordination Chemistry

Inorganic Chemistry

Lithium-ion batteries

Metal-ion batteries

Nanotechnology

Sodium-ion batteries

1 citation

Prikhodchenko Petr

DSc in Chemistry

115 publications, 2 813 citations, 29 reviews

h-index: 29

Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences

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Ellerby L. M. et al. Copper−Zinc Superoxide Dismutase: Why Not pH-Dependent? // Journal of the American Chemical Society. 1996. Vol. 118. No. 28. pp. 6556-6561.

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Ellerby L. M., Cabelli D. E., Graden J. A., Valentine J. S. Copper−Zinc Superoxide Dismutase: Why Not pH-Dependent? // Journal of the American Chemical Society. 1996. Vol. 118. No. 28. pp. 6556-6561.

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