Characterization of DNA Probes Immobilized on Gold Surfaces
Publication type: Journal Article
Publication date: 1997-09-01
scimago Q1
wos Q1
SJR: 5.554
CiteScore: 22.5
Impact factor: 15.6
ISSN: 00027863, 15205126
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Abstract
We have characterized thiol-derivatized, single-stranded DNA (5‘-HS-(CH2)6-CAC GAC GTT GTA AAA CGA CGG CCA G-3‘, abbreviated HS-ssDNA) attached to gold via a sulfur−gold linkage using X-ray photoelectron spectroscopy (XPS), ellipsometry, and 32P-radiolabeling experiments. We found that hybridization of surface-bound HS-ssDNA is dependent on surface coverage. The buffer concentration of the HS-ssDNA solution was found to have a profound effect on surface coverage, with adsorption greatly reduced at low salt concentration. More precise control over surface coverage was achieved by creating mixed monolayers of the thiol-derivatized probe and a spacer thiol, mercaptohexanol (MCH), by way of a two-step method, where first the gold substrate is exposed to a micromolar solution of HS-ssDNA, followed by exposure to a millimolar solution of MCH. A primary advantage of using this two-step process to form HS-ssDNA/MCH mixed monolayers is that nonspecifically adsorbed DNA is largely removed from the surface. Thus, th...
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Total citations:
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Citations from 2024:
62
(4.61%)
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GOST
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Herne T. M., Tarlov M. J. Characterization of DNA Probes Immobilized on Gold Surfaces // Journal of the American Chemical Society. 1997. Vol. 119. No. 38. pp. 8916-8920.
GOST all authors (up to 50)
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Herne T. M., Tarlov M. J. Characterization of DNA Probes Immobilized on Gold Surfaces // Journal of the American Chemical Society. 1997. Vol. 119. No. 38. pp. 8916-8920.
Cite this
RIS
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TY - JOUR
DO - 10.1021/ja9719586
UR - https://doi.org/10.1021/ja9719586
TI - Characterization of DNA Probes Immobilized on Gold Surfaces
T2 - Journal of the American Chemical Society
AU - Herne, Tonya M
AU - Tarlov, Michael J
PY - 1997
DA - 1997/09/01
PB - American Chemical Society (ACS)
SP - 8916-8920
IS - 38
VL - 119
SN - 0002-7863
SN - 1520-5126
ER -
Cite this
BibTex (up to 50 authors)
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@article{1997_Herne,
author = {Tonya M Herne and Michael J Tarlov},
title = {Characterization of DNA Probes Immobilized on Gold Surfaces},
journal = {Journal of the American Chemical Society},
year = {1997},
volume = {119},
publisher = {American Chemical Society (ACS)},
month = {sep},
url = {https://doi.org/10.1021/ja9719586},
number = {38},
pages = {8916--8920},
doi = {10.1021/ja9719586}
}
Cite this
MLA
Copy
Herne, Tonya M., and Michael J Tarlov. “Characterization of DNA Probes Immobilized on Gold Surfaces.” Journal of the American Chemical Society, vol. 119, no. 38, Sep. 1997, pp. 8916-8920. https://doi.org/10.1021/ja9719586.