Membrane-Bound Molecular Rotors Measure Viscosity in Live Cells via Fluorescence Lifetime Imaging
James A. Levitt
1
,
Marina K. Kuimova
1
,
Gokhan Yahioglu
1
,
Pei-Hua Chung
1
,
Klaus Suhling
1
,
David Phillips
1
Publication type: Journal Article
Publication date: 2009-04-24
scimago Q1
wos Q3
SJR: 0.914
CiteScore: 6.2
Impact factor: 3.2
ISSN: 19327447, 19327455
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Physical and Theoretical Chemistry
General Energy
Abstract
We report intracellular fluorescence lifetime imaging (FLIM) and fluorescence anisotropy measurements of two meso-substituted fluorophores based on the boron−dipyrrin (BODIPY) structure. Both dyes incorporate hydrophobic groups, which render them membrane-soluble. We have obtained values for quantum yields, radiative and nonradiative rate constants, fluorescence lifetimes, and time-resolved anisotropy data for the dyes in homogeneous methanol/glycerol solutions of varying viscosities from 0.6 to 950 cP. We find that the fluorescence lifetimes and rotational correlation times for both dyes increase with increasing viscosity, as predicted by theory. These molecules can thus serve as fluorescent molecular rotors to report on local microviscosity, including that in live cells. The dyes are readily taken up by cells as imaged using confocal fluorescence microscopy. Using FLIM, we have detected two distinct fluorescence lifetime populations for both dyes in live SK-OV-3 human ovarian carcinoma cells, correspond...
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221
Total citations:
221
Citations from 2024:
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(11.31%)
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Levitt J. A. et al. Membrane-Bound Molecular Rotors Measure Viscosity in Live Cells via Fluorescence Lifetime Imaging // Journal of Physical Chemistry C. 2009. Vol. 113. No. 27. pp. 11634-11642.
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Levitt J. A., Kuimova M. K., Yahioglu G., Chung P., Suhling K., Phillips D. Membrane-Bound Molecular Rotors Measure Viscosity in Live Cells via Fluorescence Lifetime Imaging // Journal of Physical Chemistry C. 2009. Vol. 113. No. 27. pp. 11634-11642.
Cite this
RIS
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TY - JOUR
DO - 10.1021/jp9013493
UR - https://doi.org/10.1021/jp9013493
TI - Membrane-Bound Molecular Rotors Measure Viscosity in Live Cells via Fluorescence Lifetime Imaging
T2 - Journal of Physical Chemistry C
AU - Levitt, James A.
AU - Kuimova, Marina K.
AU - Yahioglu, Gokhan
AU - Chung, Pei-Hua
AU - Suhling, Klaus
AU - Phillips, David
PY - 2009
DA - 2009/04/24
PB - American Chemical Society (ACS)
SP - 11634-11642
IS - 27
VL - 113
SN - 1932-7447
SN - 1932-7455
ER -
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BibTex (up to 50 authors)
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@article{2009_Levitt,
author = {James A. Levitt and Marina K. Kuimova and Gokhan Yahioglu and Pei-Hua Chung and Klaus Suhling and David Phillips},
title = {Membrane-Bound Molecular Rotors Measure Viscosity in Live Cells via Fluorescence Lifetime Imaging},
journal = {Journal of Physical Chemistry C},
year = {2009},
volume = {113},
publisher = {American Chemical Society (ACS)},
month = {apr},
url = {https://doi.org/10.1021/jp9013493},
number = {27},
pages = {11634--11642},
doi = {10.1021/jp9013493}
}
Cite this
MLA
Copy
Levitt, James A., et al. “Membrane-Bound Molecular Rotors Measure Viscosity in Live Cells via Fluorescence Lifetime Imaging.” Journal of Physical Chemistry C, vol. 113, no. 27, Apr. 2009, pp. 11634-11642. https://doi.org/10.1021/jp9013493.