CdSe−ZnS Quantum Dots as Resonance Energy Transfer Donors in a Model Protein−Protein Binding Assay
Publication type: Journal Article
Publication date: 2001-08-02
scimago Q1
wos Q1
SJR: 2.967
CiteScore: 14.9
Impact factor: 9.1
ISSN: 15306984, 15306992
General Chemistry
Condensed Matter Physics
General Materials Science
Mechanical Engineering
Bioengineering
Abstract
Specific binding of biotinilated bovine serum albumin (bBSA) and tetramethylrhodamine-labeled streptavidin (SAv−TMR) was observed by conjugating bBSA to CdSe−ZnS core−shell quantum dots (QDs) and observing enhanced TMR fluorescence caused by fluorescence resonance energy transfer (FRET) from the QD donors to the TMR acceptors. Because of the broad absorption spectrum of the QDs, efficient donor excitation could occur at a wavelength that was well resolved from the absorption spectrum of the acceptor, thereby minimizing direct acceptor excitation. Appreciable overlap of the donor emission and acceptor absorption spectra was achieved by size-tuning the QD emission spectrum into resonance with the acceptor absorption spectrum, and cross-talk between the donor and acceptor emission was minimized because of the narrow, symmetrically shaped QD emission spectrum. Evidence for an additional, nonspecific QD−TMR energy transfer mechanism that caused quenching of the QD emission without a corresponding TMR fluorescence enhancement was also observed.
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434
Total citations:
434
Citations from 2024:
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(1.61%)
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Willard D. M. et al. CdSe−ZnS Quantum Dots as Resonance Energy Transfer Donors in a Model Protein−Protein Binding Assay // Nano Letters. 2001. Vol. 1. No. 9. pp. 469-474.
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Willard D. M., Carillo L. L., Jung J., Van Orden A. CdSe−ZnS Quantum Dots as Resonance Energy Transfer Donors in a Model Protein−Protein Binding Assay // Nano Letters. 2001. Vol. 1. No. 9. pp. 469-474.
Cite this
RIS
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TY - JOUR
DO - 10.1021/nl015565n
UR - https://doi.org/10.1021/nl015565n
TI - CdSe−ZnS Quantum Dots as Resonance Energy Transfer Donors in a Model Protein−Protein Binding Assay
T2 - Nano Letters
AU - Willard, Dale M
AU - Carillo, Lori L
AU - Jung, Jaemyeong
AU - Van Orden, Alan
PY - 2001
DA - 2001/08/02
PB - American Chemical Society (ACS)
SP - 469-474
IS - 9
VL - 1
SN - 1530-6984
SN - 1530-6992
ER -
Cite this
BibTex (up to 50 authors)
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@article{2001_Willard,
author = {Dale M Willard and Lori L Carillo and Jaemyeong Jung and Alan Van Orden},
title = {CdSe−ZnS Quantum Dots as Resonance Energy Transfer Donors in a Model Protein−Protein Binding Assay},
journal = {Nano Letters},
year = {2001},
volume = {1},
publisher = {American Chemical Society (ACS)},
month = {aug},
url = {https://doi.org/10.1021/nl015565n},
number = {9},
pages = {469--474},
doi = {10.1021/nl015565n}
}
Cite this
MLA
Copy
Willard, Dale M., et al. “CdSe−ZnS Quantum Dots as Resonance Energy Transfer Donors in a Model Protein−Protein Binding Assay.” Nano Letters, vol. 1, no. 9, Aug. 2001, pp. 469-474. https://doi.org/10.1021/nl015565n.