Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media
Publication type: Journal Article
Publication date: 2000-01-01
scimago Q2
wos Q3
SJR: 0.528
CiteScore: 5.4
Impact factor: 2.1
ISSN: 01415492, 15736776
General Medicine
Applied Microbiology and Biotechnology
Biotechnology
Bioengineering
Abstract
Hog pancreas lipase was covalently attached to the beads of poly(vinyl alcohol)-cryogel – a macroporous hydrogel prepared by means of freeze-thaw technique. The immobilized biocatalyst thus obtained was examined in the reaction of enantioselective hydrolysis of the ethyl ester of N-benzylidene derivative of DL-phehylalanine in the medium of acetonitrile (contained 5 vol.% of water without any buffers). Eighty-three %-enantiomeric excess of the l-amino acid was reached after 144 h. Virtually the same result was obtained in the repeated use of the same immobilized biocatalyst after its 6-months-storing in a refrigerator.
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38
Total citations:
38
Citations from 2025:
1
(2.63%)
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BibTex |
MLA
Cite this
GOST
Copy
Plieva F. M. et al. Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media // Biotechnology Letters. 2000. Vol. 22. No. 7. pp. 551-554.
GOST all authors (up to 50)
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Plieva F. M., Kochetkov K. A., Singh I., Parmar V. S., Belokon Y. N., Lozinsky V. I. Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media // Biotechnology Letters. 2000. Vol. 22. No. 7. pp. 551-554.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1023/a:1005660125927
UR - https://doi.org/10.1023/a:1005660125927
TI - Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media
T2 - Biotechnology Letters
AU - Plieva, F M
AU - Kochetkov, K A
AU - Singh, Ishwar
AU - Parmar, V. S.
AU - Belokon, Yu. N.
AU - Lozinsky, V I
PY - 2000
DA - 2000/01/01
PB - Springer Nature
SP - 551-554
IS - 7
VL - 22
SN - 0141-5492
SN - 1573-6776
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2000_Plieva,
author = {F M Plieva and K A Kochetkov and Ishwar Singh and V. S. Parmar and Yu. N. Belokon and V I Lozinsky},
title = {Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media},
journal = {Biotechnology Letters},
year = {2000},
volume = {22},
publisher = {Springer Nature},
month = {jan},
url = {https://doi.org/10.1023/a:1005660125927},
number = {7},
pages = {551--554},
doi = {10.1023/a:1005660125927}
}
Cite this
MLA
Copy
Plieva, F. M., et al. “Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media.” Biotechnology Letters, vol. 22, no. 7, Jan. 2000, pp. 551-554. https://doi.org/10.1023/a:1005660125927.