Nature, volume 606, issue 7914, pages 603-608
Mechanism of mitoribosomal small subunit biogenesis and preinitiation
Itoh Yuzuru
1
,
Khawaja Anas
2, 3
,
Laptev Ivan G.
4, 5, 6
,
Cipullo Miriam
2, 3
,
Atanassov Ilian
7
,
Sergiev Petr V.
4, 5, 6, 8
,
Rorbach Joanna
2, 3
,
6
7
Proteomics Core Facility, Max-Planck-Institute for Biology of Ageing, Cologne, Germany
|
Publication type: Journal Article
Publication date: 2022-06-08
PubMed ID:
35676484
Multidisciplinary
Abstract
Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. 1) outcompetes RBFA to complete the assembly with the SSU–mS37–mtIF3 complex2 that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Itoh Y. et al. Mechanism of mitoribosomal small subunit biogenesis and preinitiation // Nature. 2022. Vol. 606. No. 7914. pp. 603-608.
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Itoh Y., Khawaja A., Laptev I. G., Cipullo M., Atanassov I., Sergiev P. V., Rorbach J., Amunts A. Mechanism of mitoribosomal small subunit biogenesis and preinitiation // Nature. 2022. Vol. 606. No. 7914. pp. 603-608.
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TY - JOUR
DO - 10.1038/s41586-022-04795-x
UR - https://doi.org/10.1038%2Fs41586-022-04795-x
TI - Mechanism of mitoribosomal small subunit biogenesis and preinitiation
T2 - Nature
AU - Itoh, Yuzuru
AU - Khawaja, Anas
AU - Laptev, Ivan G.
AU - Cipullo, Miriam
AU - Atanassov, Ilian
AU - Sergiev, Petr V.
AU - Rorbach, Joanna
AU - Amunts, Alexey
PY - 2022
DA - 2022/06/08 00:00:00
PB - Springer Nature
SP - 603-608
IS - 7914
VL - 606
PMID - 35676484
SN - 0028-0836
SN - 1476-4687
ER -
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@article{2022_Itoh,
author = {Yuzuru Itoh and Anas Khawaja and Ivan G. Laptev and Miriam Cipullo and Ilian Atanassov and Petr V. Sergiev and Joanna Rorbach and Alexey Amunts},
title = {Mechanism of mitoribosomal small subunit biogenesis and preinitiation},
journal = {Nature},
year = {2022},
volume = {606},
publisher = {Springer Nature},
month = {jun},
url = {https://doi.org/10.1038%2Fs41586-022-04795-x},
number = {7914},
pages = {603--608},
doi = {10.1038/s41586-022-04795-x}
}
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Itoh, Yuzuru, et al. “Mechanism of mitoribosomal small subunit biogenesis and preinitiation.” Nature, vol. 606, no. 7914, Jun. 2022, pp. 603-608. https://doi.org/10.1038%2Fs41586-022-04795-x.
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