Open Access
Scientific Reports, volume 7, issue 1, publication number 10194
Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι
Publication type: Journal Article
Publication date: 2017-08-31
PubMed ID:
28860552
Multidisciplinary
Abstract
Besides X-family DNA polymerases (first of all, Pol β) several other human DNA polymerases from Y- and A- families were shown to possess the dRP-lyase activity and could serve as backup polymerases in base excision repair (Pol ι, Rev1, Pol γ and Pol θ). However the exact position of the active sites and the amino acid residues involved in the dRP-lyase activity in Y- and A- family DNA polymerases are not known. Here we carried out functional analysis of fifteen amino acid residues possibly involved in the dRP-lyase activity of human Pol ι. We show that substitutions of residues Q59, K60 and K207 impair the dRP-lyase activity of Pol ι while residues in the HhH motif of the thumb domain are dispensable for this activity. While both K60G and K207A substitutions decrease Schiff-base intermediate formation during dRP group cleavage, the latter substitution also strongly affects the DNA polymerase activity of Pol ι, suggesting that it may impair DNA binding. These data are consistent with an important role of the N-terminal region in the dRP-lyase activity of Pol ι, with possible involvement of residues from the finger domain in the dRP group cleavage.
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Miropolskaya N. et al. Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι // Scientific Reports. 2017. Vol. 7. No. 1. 10194
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Miropolskaya N., Petushkov I., Kulbachinskiy A., Makarova A. V. Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι // Scientific Reports. 2017. Vol. 7. No. 1. 10194
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TY - JOUR
DO - 10.1038/s41598-017-10668-5
UR - https://doi.org/10.1038%2Fs41598-017-10668-5
TI - Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι
T2 - Scientific Reports
AU - Miropolskaya, Nataliya
AU - Petushkov, Ivan
AU - Kulbachinskiy, Andrey
AU - Makarova, Alena V.
PY - 2017
DA - 2017/08/31 00:00:00
PB - Springer Nature
IS - 1
VL - 7
PMID - 28860552
SN - 2045-2322
ER -
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@article{2017_Miropolskaya,
author = {Nataliya Miropolskaya and Ivan Petushkov and Andrey Kulbachinskiy and Alena V. Makarova},
title = {Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι},
journal = {Scientific Reports},
year = {2017},
volume = {7},
publisher = {Springer Nature},
month = {aug},
url = {https://doi.org/10.1038%2Fs41598-017-10668-5},
number = {1},
doi = {10.1038/s41598-017-10668-5}
}
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