Laboratory of mechanisms of replication of damaged DNA
Authorization required.
The group studies the response of cells to DNA damage and the mechanisms of replication of damaged DNA with the participation of highly error-prone specialized human DNA polymerases (specDNCP): Pol iota, Pol eta, Pol zeta, PrimPol. The main areas of research:
- Efficiency and accuracy of DNA synthesis on DNA matrices with different types of damage
- The structural and functional organization of the educational centers of the Special Scientific and Technical Commission
- Regulation of activity and switching of special equipment
- The effect of mutations and polymorphisms (primarily found in cancer patients) on the activity of special DNCP
- Preparation of inhibitor aptamers for special DNCP
- Recombinant protein expression
- Affinity chromatography
- PCR
- Site-directed mutagenesis
- Selection of DNA aptamers for biological targets
- CRISPR-Cas
- In vitro testing of DNA polymerase activity
- Cell and tissue culture
- Isolation and purification of target proteins
Research directions
Analysis of DNA polymerase activity in vitro
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Investigation of the efficiency and accuracy of DNA synthesis on DNA matrices with different types of damage
Publications and patents
Алена Владимировна Макарова, Яна Германовна Калюжная, Ксения Александровна Бондаренко
RU2770691,
2021
2022
—
2023
| Болдинова Елизавета Олеговна
2022
—
2023
| Шилкин Евгений Сергеевич
2022
—
2023
| Столяренко Анастасия Дмитриевна
2018
—
2020
| Макарова Алена Владимировна
Lab address
Москва, пл. Академика Курчатова, 2
Authorization required.