Open Access
Scientific Reports, volume 11, issue 1, publication number 17588
Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links
Yudkina Anna V
2
,
Shilkin Evgeniy S.
1
,
Gagarinskaya Diana I
1
,
Baranovskiy Andrey G.
3
,
Tahirov Tahir H.
3
,
Zharkov Dmitry O.
2, 4
,
1
3
Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, USA
|
Publication type: Journal Article
Publication date: 2021-09-02
PubMed ID:
34475447
Multidisciplinary
Abstract
Human PrimPol belongs to the archaeo-eukaryotic primase superfamily of primases and is involved in de novo DNA synthesis downstream of blocking DNA lesions and non-B DNA structures. PrimPol possesses both DNA/RNA primase and DNA polymerase activities, and also bypasses a number of DNA lesions in vitro. In this work, we have analyzed translesion synthesis activity of PrimPol in vitro on DNA with an 1,2-intrastrand cisplatin cross-link (1,2-GG CisPt CL) or a model DNA–protein cross-link (DpCL). PrimPol was capable of the 1,2-GG CisPt CL bypass in the presence of Mn2+ ions and preferentially incorporated two complementary dCMPs opposite the lesion. Nucleotide incorporation was stimulated by PolDIP2, and yeast Pol ζ efficiently extended from the nucleotides inserted opposite the 1,2-GG CisPt CL in vitro. DpCLs significantly blocked the DNA polymerase activity and strand displacement synthesis of PrimPol. However, PrimPol was able to reach the DpCL site in single strand template DNA in the presence of both Mg2+ and Mn2+ ions despite the presence of the bulky protein obstacle.
Citations by journals
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1
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Genes
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1 publication, 7.14%
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1 publication, 7.14%
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1
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Citations by publishers
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1
2
3
4
5
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5 publications, 35.71%
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2 publications, 14.29%
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Pleiades Publishing
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2 publications, 14.29%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
1 publication, 7.14%
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American Association for the Advancement of Science (AAAS)
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American Association for the Advancement of Science (AAAS)
1 publication, 7.14%
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Springer Nature
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Springer Nature
1 publication, 7.14%
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Elsevier
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Elsevier
1 publication, 7.14%
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1
2
3
4
5
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Boldinova E. O. et al. Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links // Scientific Reports. 2021. Vol. 11. No. 1. 17588
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Boldinova E. O., Yudkina A. V., Shilkin E. S., Gagarinskaya D. I., Baranovskiy A. G., Tahirov T. H., Zharkov D. O., Makarova A. V. Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links // Scientific Reports. 2021. Vol. 11. No. 1. 17588
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TY - JOUR
DO - 10.1038/s41598-021-96692-y
UR - https://doi.org/10.1038%2Fs41598-021-96692-y
TI - Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links
T2 - Scientific Reports
AU - Boldinova, Elizaveta O
AU - Yudkina, Anna V
AU - Shilkin, Evgeniy S.
AU - Gagarinskaya, Diana I
AU - Baranovskiy, Andrey G.
AU - Tahirov, Tahir H.
AU - Zharkov, Dmitry O.
AU - Makarova, Alena V.
PY - 2021
DA - 2021/09/02 00:00:00
PB - Springer Nature
IS - 1
VL - 11
PMID - 34475447
SN - 2045-2322
ER -
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@article{2021_Boldinova,
author = {Elizaveta O Boldinova and Anna V Yudkina and Evgeniy S. Shilkin and Diana I Gagarinskaya and Andrey G. Baranovskiy and Tahir H. Tahirov and Dmitry O. Zharkov and Alena V. Makarova},
title = {Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links},
journal = {Scientific Reports},
year = {2021},
volume = {11},
publisher = {Springer Nature},
month = {sep},
url = {https://doi.org/10.1038%2Fs41598-021-96692-y},
number = {1},
doi = {10.1038/s41598-021-96692-y}
}