Open Access
Nature Communications, volume 13, issue 1, publication number 155
A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo
Leo Hanke
1
,
Hrishikesh Das
2
,
Daniel J. Sheward
1, 3
,
Maria Laura A Perez Vidakovics
1
,
Egon Urgard
1
,
Ainhoa Moliner Morro
1
,
Changil Kim
1
,
Vivien Karl
1
,
Alec Pankow
1
,
Natalie L Smith
1
,
Bartlomiej Porebski
4
,
Oscar Fernandez Capetillo
4, 5
,
Erdinc Sezgin
6
,
Gabriel K. Pedersen
7
,
Jonathan M. Coquet
1
,
B. Martin Hallberg
2, 8
,
Ben Murrell
1
,
Gerald M. McInerney
1
5
Genomic Instability Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain
|
8
Karolinska Institutet VR-RÅC, Centre for Structural Systems Biology, Hamburg, Germany
|
Publication type: Journal Article
Publication date: 2022-01-10
Journal:
Nature Communications
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16.6
ISSN: 20411723, 20411723
General Chemistry
General Biochemistry, Genetics and Molecular Biology
Multidisciplinary
General Physics and Astronomy
Abstract
Antibodies binding to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike have therapeutic promise, but emerging variants show the potential for virus escape. This emphasizes the need for therapeutic molecules with distinct and novel neutralization mechanisms. Here we describe the isolation of a nanobody that interacts simultaneously with two RBDs from different spike trimers of SARS-CoV-2, rapidly inducing the formation of spike trimer–dimers leading to the loss of their ability to attach to the host cell receptor, ACE2. We show that this nanobody potently neutralizes SARS-CoV-2, including the beta and delta variants, and cross-neutralizes SARS-CoV. Furthermore, we demonstrate the therapeutic potential of the nanobody against SARS-CoV-2 and the beta variant in a human ACE2 transgenic mouse model. This naturally elicited bispecific monomeric nanobody establishes an uncommon strategy for potent inactivation of viral antigens and represents a promising antiviral against emerging SARS-CoV-2 variants. Here, the authors isolate and characterize a bispecific monomeric nanobody that induces dimerization of SARS-CoV-2 spike trimers, neutralizes variants of concerns as well as SARS-CoV, and inhibits SARS-CoV-2 infection in mice.
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3
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Citations by publishers
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2
4
6
8
10
12
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Springer Nature
11 publications, 19.64%
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9 publications, 16.07%
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Cold Spring Harbor Laboratory
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American Chemical Society (ACS)
6 publications, 10.71%
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4 publications, 7.14%
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3 publications, 5.36%
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2 publications, 3.57%
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Proceedings of the National Academy of Sciences (PNAS)
1 publication, 1.79%
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Wiley
1 publication, 1.79%
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Acta Naturae Ltd
1 publication, 1.79%
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Pleiades Publishing
1 publication, 1.79%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 1.79%
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2
4
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8
10
12
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Hanke L. et al. A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo // Nature Communications. 2022. Vol. 13. No. 1. 155
GOST all authors (up to 50)
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Hanke L., Das H., Sheward D. J., Perez Vidakovics M. L. A., Urgard E., Moliner Morro A., Kim C., Karl V., Pankow A., Smith N. L., Porebski B., Fernandez Capetillo O., Sezgin E., Pedersen G. K., Coquet J. M., Hallberg B. M., Murrell B., McInerney G. M. A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo // Nature Communications. 2022. Vol. 13. No. 1. 155
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TY - JOUR
DO - 10.1038/s41467-021-27610-z
UR - https://doi.org/10.1038/s41467-021-27610-z
TI - A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo
T2 - Nature Communications
AU - Hanke, Leo
AU - Das, Hrishikesh
AU - Sheward, Daniel J.
AU - Perez Vidakovics, Maria Laura A
AU - Urgard, Egon
AU - Moliner Morro, Ainhoa
AU - Kim, Changil
AU - Karl, Vivien
AU - Pankow, Alec
AU - Smith, Natalie L
AU - Porebski, Bartlomiej
AU - Fernandez Capetillo, Oscar
AU - Sezgin, Erdinc
AU - Pedersen, Gabriel K.
AU - Coquet, Jonathan M.
AU - Hallberg, B. Martin
AU - Murrell, Ben
AU - McInerney, Gerald M.
PY - 2022
DA - 2022/01/10 00:00:00
PB - Springer Nature
IS - 1
VL - 13
SN - 2041-1723
SN - 2041-1723
ER -
Cite this
BibTex
Copy
@article{2022_Hanke,
author = {Leo Hanke and Hrishikesh Das and Daniel J. Sheward and Maria Laura A Perez Vidakovics and Egon Urgard and Ainhoa Moliner Morro and Changil Kim and Vivien Karl and Alec Pankow and Natalie L Smith and Bartlomiej Porebski and Oscar Fernandez Capetillo and Erdinc Sezgin and Gabriel K. Pedersen and Jonathan M. Coquet and B. Martin Hallberg and Ben Murrell and Gerald M. McInerney},
title = {A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo},
journal = {Nature Communications},
year = {2022},
volume = {13},
publisher = {Springer Nature},
month = {jan},
url = {https://doi.org/10.1038/s41467-021-27610-z},
number = {1},
doi = {10.1038/s41467-021-27610-z}
}