Nature Chemical Biology, volume 17, issue 1, pages 113-121

Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2

Colton J Bracken ¹

Shion A. Lim ¹

Paige Solomon ¹

Nicholas J Rettko ¹

P. Nguyen-Duy ^{1, 2}

Beth Shoshana Zha ³

James R. Byrnes ¹

Jie Zhou ¹

Irene Lui ¹

Jia Liu ^{1, 4}

Katarina Pance ¹

Axel F Brilot ^{1, 5, 6}

Kyle E Lopez

Arthur Melo

Frank Moss

Alexandrea N Rizo

Ming Sun

Paul V. Thomas

Feng Wang

Julian R Braxton

Un Seng Chio

Miles Sasha Dickinson

Fei Li

Junrui Li

Yanxin Liu

Henry C Nguyen

Thomas H Pospiech

Donovan Trinidad

Kaihua Zhang

Christian Billesboelle

Alisa Bowen

Melody G Campbell

Devan Diwanji

Nick Hoppe

Yen-Li Li

Phuong Nguyen

Carlos Nowotny

Cristina Puchades

Mali Safari

Smriti Sangwan

Amber M. Smith

Raphael Trenker

Natalie Whitis

Jianhua Zhao

Daniel Asarnow

Caleigh M. Azumaya

Cynthia M. Chio

Bryan Faust

Kate Kim

Michelle Moritz

Tristan W Owens

Jessica K Peters

Sergei Pourmal

Kaitlin Schaefer

Tsz Kin Martin Tsui

Justin Biel

Ishan Deshpande

Nadia Herrera

Xi Liu

Ursula Schulze-Gahmen

Iris D. Young

Jen Chen

Amy Diallo

Loan Doan

Sebastian Flores

Meghna Gupta

Mingliang Jin

Huong T Kratochvil

Victor L Lam

Yang Li

Megan Lo

Gregory E Merz

Joana Paulino

Aye C Thwin

Erron W Titus

Zanlin Yu

Fengbo Zhou

Yang Zhang

David Bulkley

Arceli Joves

Almarie Joves

Liam Mckay

Mariano Tabios

Eric Tse

David A. Agard

Yifan Cheng

James S. Fraser

Adam Frost

Natalia Jura

Tanja Kortemme

Nevan J. Krogan

Aashish Manglik

Oren S Rosenberg

Daniel R Southworth

Robert M. Stroud

Kliment A. Verba

Xin X Zhou

Kevin K. Leung

James A. Wells

Hide authors affiliations Show authors affiliations: 6 affiliations

Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, USA |

Lyell Immunopharma Inc., Seattle, USA |

Department of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California San Francisco, San Francisco, USA |

⁴

Merck & Co., South San Francisco, USA |

⁵

Department of Cellular & Molecular Pharmacology, University of California San Francisco, San Francisco, USA |

⁶

Chan Zuckerberg BioHub, San Francisco, USA |

Publication type: Journal Article

Publication date: 2020-10-20

Springer Nature

Journal: Nature Chemical Biology

Quartile SCImago

Quartile WOS

Impact factor: 14.8

ISSN: 15524450, 15524469

DOI: 10.1038/s41589-020-00679-1

Copy DOI

Molecular Biology

Cell Biology

Abstract

Neutralizing agents against SARS-CoV-2 are urgently needed for the treatment and prophylaxis of COVID-19. Here, we present a strategy to rapidly identify and assemble synthetic human variable heavy (VH) domains toward neutralizing epitopes. We constructed a VH-phage library and targeted the angiotensin-converting enzyme 2 (ACE2) binding interface of the SARS-CoV-2 Spike receptor-binding domain (Spike-RBD). Using a masked selection approach, we identified VH binders to two non-overlapping epitopes and further assembled these into multivalent and bi-paratopic formats. These VH constructs showed increased affinity to Spike (up to 600-fold) and neutralization potency (up to 1,400-fold) on pseudotyped SARS-CoV-2 virus when compared to standalone VH domains. The most potent binder, a trivalent VH, neutralized authentic SARS-CoV-2 with a half-maximal inhibitory concentration (IC50) of 4.0 nM (180 ng ml−1). A cryo-EM structure of the trivalent VH bound to Spike shows each VH domain engaging an RBD at the ACE2 binding site, confirming our original design strategy. A screening approach finds VH-domain antibodies that bind the SARS-CoV-2 Spike protein receptor-binding domain at its interface with host ACE2. Bi-paratopic and multivalent binders have high affinity and potency.

By date By citations

Top-30

Citations by journals

	1 2 3 4
Frontiers in Immunology	Frontiers in Immunology, 4, 4.82% Frontiers in Immunology 4 publications, 4.82%
mAbs	mAbs, 4, 4.82% mAbs 4 publications, 4.82%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 2.41% International Journal of Molecular Sciences 2 publications, 2.41%
Scientific Reports	Scientific Reports, 2, 2.41% Scientific Reports 2 publications, 2.41%
International Journal of Biological Macromolecules	International Journal of Biological Macromolecules, 2, 2.41% International Journal of Biological Macromolecules 2 publications, 2.41%
PLoS Pathogens	PLoS Pathogens, 2, 2.41% PLoS Pathogens 2 publications, 2.41%
Journal of the American Chemical Society	Journal of the American Chemical Society, 2, 2.41% Journal of the American Chemical Society 2 publications, 2.41%
Biochemical Journal	Biochemical Journal, 1, 1.2% Biochemical Journal 1 publication, 1.2%
Frontiers in Cellular and Infection Microbiology	Frontiers in Cellular and Infection Microbiology, 1, 1.2% Frontiers in Cellular and Infection Microbiology 1 publication, 1.2%
Microbial Cell Factories	Microbial Cell Factories, 1, 1.2% Microbial Cell Factories 1 publication, 1.2%
Virology Journal	Virology Journal, 1, 1.2% Virology Journal 1 publication, 1.2%
Nature Communications	Nature Communications, 1, 1.2% Nature Communications 1 publication, 1.2%
Molecular Biology Reports	Molecular Biology Reports, 1, 1.2% Molecular Biology Reports 1 publication, 1.2%
BioDrugs	BioDrugs, 1, 1.2% BioDrugs 1 publication, 1.2%
Communications Biology	Communications Biology, 1, 1.2% Communications Biology 1 publication, 1.2%
Nano Today	Nano Today, 1, 1.2% Nano Today 1 publication, 1.2%
Molecular Therapy	Molecular Therapy, 1, 1.2% Molecular Therapy 1 publication, 1.2%
Structure	Structure, 1, 1.2% Structure 1 publication, 1.2%
Molecular and Cellular Proteomics	Molecular and Cellular Proteomics, 1, 1.2% Molecular and Cellular Proteomics 1 publication, 1.2%
iScience	iScience, 1, 1.2% iScience 1 publication, 1.2%
Chem	Chem, 1, 1.2% Chem 1 publication, 1.2%
Biotechnology Advances	Biotechnology Advances, 1, 1.2% Biotechnology Advances 1 publication, 1.2%
PLoS ONE	PLoS ONE, 1, 1.2% PLoS ONE 1 publication, 1.2%
Journal of Biological Chemistry	Journal of Biological Chemistry, 1, 1.2% Journal of Biological Chemistry 1 publication, 1.2%
Cell	Cell, 1, 1.2% Cell 1 publication, 1.2%
Angewandte Chemie	Angewandte Chemie, 1, 1.2% Angewandte Chemie 1 publication, 1.2%
Angewandte Chemie - International Edition	Angewandte Chemie - International Edition, 1, 1.2% Angewandte Chemie - International Edition 1 publication, 1.2%
British Journal of Pharmacology	British Journal of Pharmacology, 1, 1.2% British Journal of Pharmacology 1 publication, 1.2%
Biotechnology Journal	Biotechnology Journal, 1, 1.2% Biotechnology Journal 1 publication, 1.2%
	1 2 3 4

Citations by publishers

	2 4 6 8 10 12 14
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 14, 16.87% Cold Spring Harbor Laboratory 14 publications, 16.87%
Springer Nature	Springer Nature, 12, 14.46% Springer Nature 12 publications, 14.46%
Elsevier	Elsevier, 12, 14.46% Elsevier 12 publications, 14.46%
American Chemical Society (ACS)	American Chemical Society (ACS), 7, 8.43% American Chemical Society (ACS) 7 publications, 8.43%
Frontiers Media S.A.	Frontiers Media S.A., 5, 6.02% Frontiers Media S.A. 5 publications, 6.02%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 5, 6.02% Multidisciplinary Digital Publishing Institute (MDPI) 5 publications, 6.02%
Wiley	Wiley, 5, 6.02% Wiley 5 publications, 6.02%
Taylor & Francis	Taylor & Francis, 4, 4.82% Taylor & Francis 4 publications, 4.82%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 3, 3.61% Public Library of Science (PLoS) 3 publications, 3.61%
American Society for Biochemistry and Molecular Biology	American Society for Biochemistry and Molecular Biology, 2, 2.41% American Society for Biochemistry and Molecular Biology 2 publications, 2.41%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 2, 2.41% Royal Society of Chemistry (RSC) 2 publications, 2.41%
Portland Press	Portland Press, 1, 1.2% Portland Press 1 publication, 1.2%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 1, 1.2% American Association for the Advancement of Science (AAAS) 1 publication, 1.2%
American Society for Microbiology	American Society for Microbiology, 1, 1.2% American Society for Microbiology 1 publication, 1.2%
Hindawi Limited	Hindawi Limited, 1, 1.2% Hindawi Limited 1 publication, 1.2%
eLife Sciences Publications	eLife Sciences Publications, 1, 1.2% eLife Sciences Publications 1 publication, 1.2%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 1, 1.2% Proceedings of the National Academy of Sciences (PNAS) 1 publication, 1.2%
Oxford University Press	Oxford University Press, 1, 1.2% Oxford University Press 1 publication, 1.2%
Annual Reviews	Annual Reviews, 1, 1.2% Annual Reviews 1 publication, 1.2%
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii	Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii, 1, 1.2% Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii 1 publication, 1.2%
	2 4 6 8 10 12 14

We do not take into account publications without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023,2024],"ids":[0,0,0,0,0],"codes":[0,0,0,0,0],"imageUrls":["","","","",""],"datasets":[{"label":"Citations number","data":[1,38,25,8,11],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["1.2","45.78","30.12","9.64","13.25"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Frontiers in Immunology","mAbs","International Journal of Molecular Sciences","Scientific Reports","International Journal of Biological Macromolecules","PLoS Pathogens","Journal of the American Chemical Society","Biochemical Journal","Frontiers in Cellular and Infection Microbiology","Microbial Cell Factories","Virology Journal","Nature Communications","Molecular Biology Reports","BioDrugs","Communications Biology","Nano Today","Molecular Therapy","Structure","Molecular and Cellular Proteomics","iScience","Chem","Biotechnology Advances","PLoS ONE","Journal of Biological Chemistry","Cell","Angewandte Chemie","Angewandte Chemie - International Edition","British Journal of Pharmacology","Biotechnology Journal"],"ids":[1699,9244,14627,13767,23035,5093,4813,15459,21709,23412,22057,3231,2791,15889,11778,1433,2784,5828,6086,6073,12363,17421,24963,19451,16552,25450,19215,17686,12083],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp"],"datasets":[{"label":"","data":[4,4,2,2,2,2,2,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[4.82,4.82,2.41,2.41,2.41,2.41,2.41,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Cold Spring Harbor Laboratory","Springer Nature","Elsevier","American Chemical Society (ACS)","Frontiers Media S.A.","Multidisciplinary Digital Publishing Institute (MDPI)","Wiley","Taylor & Francis","Public Library of Science (PLoS)","American Society for Biochemistry and Molecular Biology","Royal Society of Chemistry (RSC)","Portland Press","American Association for the Advancement of Science (AAAS)","American Society for Microbiology","Hindawi Limited","eLife Sciences Publications","Proceedings of the National Academy of Sciences (PNAS)","Oxford University Press","Annual Reviews","Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii"],"ids":[6909,8,17,40,208,202,11,18,344,2090,123,801,189,142,6921,6627,162,19,6941,9422],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/hqfzhQAjTGlNSRs6yzFNITgjSMm9Jr2QuotJHIvE_medium.webp","\/storage\/images\/resized\/eIZgkq1MRZyJ3hBkdupnUUv7wgB6zCl9D99SlPN5_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp","\/storage\/images\/resized\/9Mus3KG1Tkd7Bwaurt8H3RwWh0CxRlGoO6ng9UK1_medium.webp"],"datasets":[{"label":"","data":[14,12,12,7,5,5,5,4,3,2,2,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[16.87,14.46,14.46,8.43,6.02,6.02,6.02,4.82,3.61,2.41,2.41,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2,1.2],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartiles":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,0,0,0,0],"percentage":["0","0","0","0","0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,1,1,0,0],"percentage":["0","1.2","1.2","0","0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[0,2,0,0,0],"percentage":["0","2.41","0","0","0"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[0,24,21,6,10],"percentage":["0","28.92","25.3","7.23","12.05"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[1,11,3,2,1],"percentage":["1.2","13.25","3.61","2.41","1.2"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by SCImago per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartilesWs":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,0,0,0,0],"percentage":["0","0","0","0","0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,1,1,0,0],"percentage":["0","1.2","1.2","0","0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[0,7,3,1,2],"percentage":["0","8.43","3.61","1.2","2.41"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[0,16,17,5,8],"percentage":["0","19.28","20.48","6.02","9.64"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[1,14,4,2,1],"percentage":["1.2","16.87","4.82","2.41","1.2"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by WoS per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Are you a researcher?

Create a profile to get free access to personal recommendations for colleagues and new articles.

Metrics

Cite this

GOST |

Cite this

GOST Copy

Bracken C. J. et al. Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2 // Nature Chemical Biology. 2020. Vol. 17. No. 1. pp. 113-121.

GOST all authors (up to 50) Copy

Bracken C. J. et al. Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2 // Nature Chemical Biology. 2020. Vol. 17. No. 1. pp. 113-121.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1038/s41589-020-00679-1

UR - https://doi.org/10.1038/s41589-020-00679-1

TI - Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2

T2 - Nature Chemical Biology

AU - Bracken, Colton J

AU - Lim, Shion A.

AU - Solomon, Paige

AU - Rettko, Nicholas J

AU - Nguyen-Duy, P.

AU - Zha, Beth Shoshana

AU - Schaefer, Kaitlin

AU - Byrnes, James R.

AU - Zhou, Jie

AU - Lui, Irene

AU - Liu, Jia

AU - Pance, Katarina

AU - Azumaya, Caleigh M.

AU - Braxton, Julian R

AU - Brilot, Axel F

AU - Gupta, Meghna

AU - Li, Fei

AU - Lopez, Kyle E

AU - Melo, Arthur

AU - Merz, Gregory E

AU - Moss, Frank

AU - Paulino, Joana

AU - Pospiech, Thomas H

AU - Pourmal, Sergei

AU - Puchades, Cristina

AU - Rizo, Alexandrea N

AU - Smith, Amber M.

AU - Sun, Ming

AU - Thomas, Paul V.

AU - Wang, Feng

AU - Yu, Zanlin

AU - Asarnow, Daniel

AU - Campbell, Melody G

AU - Chio, Cynthia M.

AU - Chio, Un Seng

AU - Dickinson, Miles Sasha

AU - Diwanji, Devan

AU - Faust, Bryan

AU - Hoppe, Nick

AU - Jin, Mingliang

AU - Li, Junrui

AU - Liu, Yanxin

AU - Nguyen, Henry C

AU - Sangwan, Smriti

AU - Trenker, Raphael

AU - Trinidad, Donovan

AU - Tse, Eric

AU - Zhang, Kaihua

AU - Zhou, Fengbo

AU - Billesboelle, Christian

AU - Bowen, Alisa

AU - Li, Yen-Li

AU - Nguyen, Phuong

AU - Nowotny, Carlos

AU - Safari, Mali

AU - Tsui, Tsz Kin Martin

AU - Whitis, Natalie

AU - Zhao, Jianhua

AU - Kim, Kate

AU - Moritz, Michelle

AU - Owens, Tristan W

AU - Peters, Jessica K

AU - Biel, Justin

AU - Deshpande, Ishan

AU - Herrera, Nadia

AU - Kratochvil, Huong T

AU - Liu, Xi

AU - Schulze-Gahmen, Ursula

AU - Young, Iris D.

AU - Chen, Jen

AU - Diallo, Amy

AU - Doan, Loan

AU - Flores, Sebastian

AU - Lam, Victor L

AU - Li, Yang

AU - Lo, Megan

AU - Thwin, Aye C

AU - Titus, Erron W

AU - Zhang, Yang

AU - Bulkley, David

AU - Joves, Arceli

AU - Joves, Almarie

AU - Mckay, Liam

AU - Tabios, Mariano

AU - Agard, David A.

AU - Cheng, Yifan

AU - Fraser, James S.

AU - Frost, Adam

AU - Jura, Natalia

AU - Kortemme, Tanja

AU - Krogan, Nevan J.

AU - Manglik, Aashish

AU - Rosenberg, Oren S

AU - Southworth, Daniel R

AU - Stroud, Robert M.

AU - Verba, Kliment A.

AU - Zhou, Xin X

AU - Leung, Kevin K.

AU - Wells, James A.

PY - 2020

DA - 2020/10/20 00:00:00

PB - Springer Nature

SP - 113-121

IS - 1

VL - 17

SN - 1552-4450

SN - 1552-4469

ER -

BibTex |

Cite this

BibTex Copy

@article{2020_Bracken,

author = {Colton J Bracken and Shion A. Lim and Paige Solomon and Nicholas J Rettko and P. Nguyen-Duy and Beth Shoshana Zha and Kaitlin Schaefer and James R. Byrnes and Jie Zhou and Irene Lui and Jia Liu and Katarina Pance and Caleigh M. Azumaya and Julian R Braxton and Axel F Brilot and Meghna Gupta and Fei Li and Kyle E Lopez and Arthur Melo and Gregory E Merz and Frank Moss and Joana Paulino and Thomas H Pospiech and Sergei Pourmal and Cristina Puchades and Alexandrea N Rizo and Amber M. Smith and Ming Sun and Paul V. Thomas and Feng Wang and Zanlin Yu and Daniel Asarnow and Melody G Campbell and Cynthia M. Chio and Un Seng Chio and Miles Sasha Dickinson and Devan Diwanji and Bryan Faust and Nick Hoppe and Mingliang Jin and Junrui Li and Yanxin Liu and Henry C Nguyen and Smriti Sangwan and Raphael Trenker and Donovan Trinidad and Eric Tse and Kaihua Zhang and Fengbo Zhou and Christian Billesboelle and Alisa Bowen and Yen-Li Li and Phuong Nguyen and Carlos Nowotny and Mali Safari and Tsz Kin Martin Tsui and Natalie Whitis and Jianhua Zhao and Kate Kim and Michelle Moritz and Tristan W Owens and Jessica K Peters and Justin Biel and Ishan Deshpande and Nadia Herrera and Huong T Kratochvil and Xi Liu and Ursula Schulze-Gahmen and Iris D. Young and Jen Chen and Amy Diallo and Loan Doan and Sebastian Flores and Victor L Lam and Yang Li and Megan Lo and Aye C Thwin and Erron W Titus and Yang Zhang and David Bulkley and Arceli Joves and Almarie Joves and Liam Mckay and Mariano Tabios and David A. Agard and Yifan Cheng and James S. Fraser and Adam Frost and Natalia Jura and Tanja Kortemme and Nevan J. Krogan and Aashish Manglik and Oren S Rosenberg and Daniel R Southworth and Robert M. Stroud and Kliment A. Verba and Xin X Zhou and Kevin K. Leung and James A. Wells},

title = {Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2},

journal = {Nature Chemical Biology},

year = {2020},

volume = {17},

publisher = {Springer Nature},

month = {oct},

url = {https://doi.org/10.1038/s41589-020-00679-1},

number = {1},

pages = {113--121},

doi = {10.1038/s41589-020-00679-1}

}

MLA

Cite this

MLA Copy

Bracken, Colton J., et al. “Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2.” Nature Chemical Biology, vol. 17, no. 1, Oct. 2020, pp. 113-121. https://doi.org/10.1038/s41589-020-00679-1.

Found error?

Publisher

Springer Nature

Journal

Nature Chemical Biology

Quartile SCImago

Quartile WOS

Impact factor

14.8

ISSN

15524450 (Print)
15524469 (Electronic)