Fluorescence microscopy today

Rafael Yuste ¹

Hide authors affiliations Show authors affiliations: 1 affiliation

the Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, USA

Columbia University

Howard Hughes Medical Institute

Publication type: Journal Article

Publication date: 2005-12-01

Springer Nature

Nature Methods

scimago Q1

wos Q1

SJR: 17.251

CiteScore: 49.0

Impact factor: 32.1

ISSN: 15487091, 15487105

DOI: 10.1038/nmeth1205-902

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PubMed ID: 16299474

Biochemistry

Molecular Biology

Cell Biology

Biotechnology

Abstract

Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear and hyper-resolution microscopies are poised to profoundly transform many fields of biological research.

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Mohr Gerhard

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h-index: 38

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Muller Werner

professor, full member of the Academia Europaea

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University of Manchester

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