Open Access
Nature Communications, volume 13, issue 1, publication number 507
Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore
Tau Omri
1
,
Henley Alice
1
,
Boichenko Anton N.
2
,
Kleshchina Nadezhda N
2
,
Riley River
1
,
Wang Bingxing
1, 3
,
Winning Danielle
1
,
Lewin Ross
1
,
Parkin Ivan P
1
,
Ward John M
4
,
Hailes Helen C
1
,
3
College of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang, China
|
Publication type: Journal Article
Publication date: 2022-01-26
Journal:
Nature Communications
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16.6
ISSN: 20411723
General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy
Abstract
Green fluorescent protein (GFP), the most widely used fluorescent protein for in vivo monitoring of biological processes, is known to undergo photooxidation reactions. However, the most fundamental property underpinning photooxidation, the electron detachment energy, has only been measured for the deprotonated GFP chromophore in the gas phase. Here, we use multiphoton ultraviolet photoelectron spectroscopy in a liquid-microjet and high-level quantum chemistry calculations to determine the electron detachment energy of the GFP chromophore in aqueous solution. The aqueous environment is found to raise the detachment energy by around 4 eV compared to the gas phase, similar to calculations of the chromophore in its native protein environment. In most cases, electron detachment is found to occur resonantly through electronically excited states of the chromophore, highlighting their importance in photo-induced electron transfer processes in the condensed phase. Our results suggest that the photooxidation properties of the GFP chromophore in an aqueous environment will be similar to those in the protein.
Citations by journals
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3
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Journal of Physical Chemistry Letters
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Journal of Physical Chemistry Letters
3 publications, 30%
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Accounts of Chemical Research
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2 publications, 20%
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Journal of Physical Chemistry A
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Journal of Physical Chemistry A
1 publication, 10%
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Journal of Chemical Theory and Computation
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Journal of Chemical Theory and Computation
1 publication, 10%
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Chemical Science
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Chemical Science
1 publication, 10%
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Journal of Chromatography A
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Journal of Chromatography A
1 publication, 10%
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1
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3
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Citations by publishers
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7
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American Chemical Society (ACS)
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American Chemical Society (ACS)
7 publications, 70%
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Royal Society of Chemistry (RSC)
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Royal Society of Chemistry (RSC)
1 publication, 10%
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Elsevier
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Elsevier
1 publication, 10%
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1
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7
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Tau O. et al. Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore // Nature Communications. 2022. Vol. 13. No. 1. 507
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Tau O., Henley A., Boichenko A. N., Kleshchina N. N., Riley R., Wang B., Winning D., Lewin R., Parkin I. P., Ward J. M., Hailes H. C., Bochenkova A. V., Fielding H. H. Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore // Nature Communications. 2022. Vol. 13. No. 1. 507
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TY - JOUR
DO - 10.1038/s41467-022-28155-5
UR - https://doi.org/10.1038%2Fs41467-022-28155-5
TI - Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore
T2 - Nature Communications
AU - Tau, Omri
AU - Henley, Alice
AU - Boichenko, Anton N.
AU - Kleshchina, Nadezhda N
AU - Riley, River
AU - Wang, Bingxing
AU - Winning, Danielle
AU - Lewin, Ross
AU - Parkin, Ivan P
AU - Ward, John M
AU - Hailes, Helen C
AU - Bochenkova, Anastasia V.
AU - Fielding, H. H.
PY - 2022
DA - 2022/01/26 00:00:00
PB - Springer Nature
IS - 1
VL - 13
SN - 2041-1723
ER -
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@article{2022_Tau,
author = {Omri Tau and Alice Henley and Anton N. Boichenko and Nadezhda N Kleshchina and River Riley and Bingxing Wang and Danielle Winning and Ross Lewin and Ivan P Parkin and John M Ward and Helen C Hailes and Anastasia V. Bochenkova and H. H. Fielding},
title = {Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore},
journal = {Nature Communications},
year = {2022},
volume = {13},
publisher = {Springer Nature},
month = {jan},
url = {https://doi.org/10.1038%2Fs41467-022-28155-5},
number = {1},
doi = {10.1038/s41467-022-28155-5}
}