Nature Methods

, volume 17 , issue 1 , pages 55-58

mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM

Zhifei Fu ^{1, 2}

Dingming Peng ^{1, 2}

Mingshu Zhang ¹

Fudong Xue ¹

Rui Zhang ³

Wenting He ¹

Tao Xu ^{2, 3}

Pingyong Xu ^{1, 2, 3}

Hide authors affiliations Show authors affiliations: 3 affiliations

Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China |

College of life sciences, University of Chinese Academy of Sciences, Beijing, China |

National Laboratory of Biomacromolecules, CAS Center for excellence in biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China |

Publication type: Journal Article

Publication date: 2019-10-14

Springer Nature

Nature Methods

scimago Q1

wos Q1

SJR: 17.251

CiteScore: 49.0

Impact factor: 32.1

ISSN: 15487091, 15487105

DOI: 10.1038/s41592-019-0613-6

Copy DOI

PubMed ID: 31611693

Biochemistry

Molecular Biology

Cell Biology

Biotechnology

Abstract

Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO4) treatment for improved SR-CLEM. mEosEM survives 1% OsO4 treatment and Epon embedding for improved super-resolution correlative light and electron microscopy.

Found

1 citation

Boyko Konstantin

🥼 🤝

PhD in Biological/biomedical sciences

125 publications, 1 281 citations, 1 review

h-index: 22

Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Research interests

Bioinformatics

Structural Biology

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GOST |

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GOST Copy

Fu Z. et al. mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM // Nature Methods. 2019. Vol. 17. No. 1. pp. 55-58.

GOST all authors (up to 50) Copy

Fu Z., Peng D., Zhang M., Xue F., Zhang R., He W., Xu T., Xu P. mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM // Nature Methods. 2019. Vol. 17. No. 1. pp. 55-58.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1038/s41592-019-0613-6

UR - https://www.nature.com/articles/s41592-019-0613-6

TI - mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM

T2 - Nature Methods

AU - Fu, Zhifei

AU - Peng, Dingming

AU - Zhang, Mingshu

AU - Xue, Fudong

AU - Zhang, Rui

AU - He, Wenting

AU - Xu, Tao

AU - Xu, Pingyong

PY - 2019

DA - 2019/10/14

PB - Springer Nature

SP - 55-58

IS - 1

VL - 17

PMID - 31611693

SN - 1548-7091

SN - 1548-7105

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2019_Fu,

author = {Zhifei Fu and Dingming Peng and Mingshu Zhang and Fudong Xue and Rui Zhang and Wenting He and Tao Xu and Pingyong Xu},

title = {mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM},

journal = {Nature Methods},

year = {2019},

volume = {17},

publisher = {Springer Nature},

month = {oct},

url = {https://www.nature.com/articles/s41592-019-0613-6},

number = {1},

pages = {55--58},

doi = {10.1038/s41592-019-0613-6}

}

MLA

Cite this

MLA Copy

Fu, Zhifei, et al. “mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM.” Nature Methods, vol. 17, no. 1, Oct. 2019, pp. 55-58. https://www.nature.com/articles/s41592-019-0613-6.

Publisher

Springer Nature

Journal

Nature Methods

scimago Q1

wos Q1

SJR

17.251

CiteScore

49.0

Impact factor

32.1

ISSN

15487091 (Print)

15487105 (Electronic)

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