Nature Methods

, том 17 , издание 1 , страницы 55-58

mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM

Zhifei Fu ^{1, 2}

Dingming Peng ^{1, 2}

Mingshu Zhang ¹

Fudong Xue ¹

Rui Zhang ³

Wenting He ¹

Tao Xu ^{2, 3}

Pingyong Xu ^{1, 2, 3}

Скрыть аффилиации авторов Показать аффилиации авторов: 3 аффилиации

Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China |

College of life sciences, University of Chinese Academy of Sciences, Beijing, China |

National Laboratory of Biomacromolecules, CAS Center for excellence in biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China |

Тип публикации: Journal Article

Дата публикации: 2019-10-14

Springer Nature

Nature Methods

scimago Q1

wos Q1

БС1

SJR: 17.251

CiteScore: 49.0

Impact factor: 32.1

ISSN: 15487091, 15487105

DOI: 10.1038/s41592-019-0613-6

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PubMed ID: 31611693

Biochemistry

Molecular Biology

Cell Biology

Biotechnology

Краткое описание

Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO4) treatment for improved SR-CLEM. mEosEM survives 1% OsO4 treatment and Epon embedding for improved super-resolution correlative light and electron microscopy.

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Fu Z. et al. mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM // Nature Methods. 2019. Vol. 17. No. 1. pp. 55-58.

ГОСТ со всеми авторами (до 50) Скопировать

Fu Z., Peng D., Zhang M., Xue F., Zhang R., He W., Xu T., Xu P. mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM // Nature Methods. 2019. Vol. 17. No. 1. pp. 55-58.

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TY - JOUR

DO - 10.1038/s41592-019-0613-6

UR - https://www.nature.com/articles/s41592-019-0613-6

TI - mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM

T2 - Nature Methods

AU - Fu, Zhifei

AU - Peng, Dingming

AU - Zhang, Mingshu

AU - Xue, Fudong

AU - Zhang, Rui

AU - He, Wenting

AU - Xu, Tao

AU - Xu, Pingyong

PY - 2019

DA - 2019/10/14

PB - Springer Nature

SP - 55-58

IS - 1

VL - 17

PMID - 31611693

SN - 1548-7091

SN - 1548-7105

ER -

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@article{2019_Fu,

author = {Zhifei Fu and Dingming Peng and Mingshu Zhang and Fudong Xue and Rui Zhang and Wenting He and Tao Xu and Pingyong Xu},

title = {mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM},

journal = {Nature Methods},

year = {2019},

volume = {17},

publisher = {Springer Nature},

month = {oct},

url = {https://www.nature.com/articles/s41592-019-0613-6},

number = {1},

pages = {55--58},

doi = {10.1038/s41592-019-0613-6}

}

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Fu, Zhifei, et al. “mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM.” Nature Methods, vol. 17, no. 1, Oct. 2019, pp. 55-58. https://www.nature.com/articles/s41592-019-0613-6.

Издатель

Springer Nature

Журнал

Nature Methods

scimago Q1

wos Q1

БС1

SJR

17.251

CiteScore

49.0

Impact factor

32.1

ISSN

15487091 (Print)

15487105 (Electronic)

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