Open Access
Scientific Reports, volume 9, issue 1, publication number 7196
Functional duplication of Rap1 in methylotrophic yeasts
Publication type: Journal Article
Publication date: 2019-05-10
PubMed ID:
31076582
Multidisciplinary
Abstract
The telomere regulator and transcription factor Rap1 is the only telomere protein conserved in yeasts and mammals. Its functional repertoire in budding yeasts is a particularly interesting field for investigation, given the high evolutionary diversity of this group of unicellular organisms. In the methylotrophic thermotolerant species Hansenula polymorpha DL-1 the RAP1 gene is duplicated (HpRAP1A and HpRAP1B). Here, we report the functional characterization of the two paralogues from H. polymorpha DL-1. We uncover distinct (but overlapping) DNA binding preferences of HpRap1A and HpRap1B proteins. We show that only HpRap1B is able to recognize telomeric DNA directly and to protect it from excessive recombination, whereas HpRap1A is associated with subtelomere regions. Furthermore, we identify specific binding sites for both HpRap1A and HpRap1B within promoters of a large number of ribosomal protein genes (RPGs), implicating Rap1 in the control of the RP regulon in H. polymorpha. Our bioinformatic analysis suggests that RAP1 was duplicated early in the evolution of the “methylotrophs” clade, and the two genes evolved independently. Therefore, our characterization of Rap1 paralogues in H. polymorpha may be relevant to other “methylotrophs”, yielding valuable insights into the evolution of budding yeasts.
Citations by journals
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Scientific Reports
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Scientific Reports
1 publication, 20%
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eLife
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eLife
1 publication, 20%
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1 publication, 20%
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Biomolecules
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Citations by publishers
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Springer Nature
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Springer Nature
1 publication, 20%
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eLife Sciences Publications
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eLife Sciences Publications
1 publication, 20%
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Oxford University Press
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Oxford University Press
1 publication, 20%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 20%
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1
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Malyavko A. N. et al. Functional duplication of Rap1 in methylotrophic yeasts // Scientific Reports. 2019. Vol. 9. No. 1. 7196
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Malyavko A. N., Petrova O. A., Zvereva M. I., Dontsova O. A. Functional duplication of Rap1 in methylotrophic yeasts // Scientific Reports. 2019. Vol. 9. No. 1. 7196
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TY - JOUR
DO - 10.1038/s41598-019-43595-8
UR - https://doi.org/10.1038%2Fs41598-019-43595-8
TI - Functional duplication of Rap1 in methylotrophic yeasts
T2 - Scientific Reports
AU - Malyavko, Alexander N
AU - Petrova, Olga A
AU - Zvereva, Maria I.
AU - Dontsova, Olga A.
PY - 2019
DA - 2019/05/10 00:00:00
PB - Springer Nature
IS - 1
VL - 9
PMID - 31076582
SN - 2045-2322
ER -
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@article{2019_Malyavko,
author = {Alexander N Malyavko and Olga A Petrova and Maria I. Zvereva and Olga A. Dontsova},
title = {Functional duplication of Rap1 in methylotrophic yeasts},
journal = {Scientific Reports},
year = {2019},
volume = {9},
publisher = {Springer Nature},
month = {may},
url = {https://doi.org/10.1038%2Fs41598-019-43595-8},
number = {1},
doi = {10.1038/s41598-019-43595-8}
}