Open Access
Scientific Reports, volume 11, issue 1, publication number 24314
The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti
Publication type: Journal Article
Publication date: 2021-12-21
PubMed ID:
34934103
Multidisciplinary
Abstract
Biphotochromic fluorescent protein SAASoti contains five cysteine residues in its sequence and a V127T point mutation transforms it to the monomeric form, mSAASoti. These cysteine residues are located far from the chromophore and might control its properties only allosterically. The influence of individual, double and triple cysteine substitutions of mSAASoti on fluorescent parameters and phototransformation reactions (irreversible green-to-red photoconversion and reversible photoswitching) is studied. A set of mSAASoti mutant forms (C21N, C117S, C71V, C105V, C175A, C21N/C71V, C21N/C175A, C21N/C71G/C175A) is obtained by site-directed mutagenesis. We demonstrate that the C21N variant exists in a monomeric form up to high concentrations, the C71V substitution accelerates photoconversion to the red form and the C105V variant has the maximum photoswitching rate. All C175A-containing variants demonstrate different photoswitching kinetics and decreased photostability during subsequent switching cycles compared with other considered systems. Classical molecular dynamic simulations reveal that the F177 side chain located in the vicinity of the chromophore is considerably more flexible in the mSAASoti compared with its C175A variant. This might be the explanation of the experimentally observed slowdown the thermal relaxation rate, i.e., trans–cis isomerization of the chromophore in mSAASoti upon C175A substitution.
Citations by journals
|
1
|
|
|
Scientific Reports
|
Scientific Reports
1 publication, 20%
|
|
Materials
|
Materials
1 publication, 20%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
1 publication, 20%
|
|
1
|
Citations by publishers
|
1
2
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 40%
|
|
Springer Nature
|
Springer Nature
1 publication, 20%
|
|
1
2
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2022,2023],"ids":[0,0],"codes":[0,0],"imageUrls":["",""],"datasets":[{"label":"Citations number","data":[4,1],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":["80","20"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Scientific Reports","Materials","International Journal of Molecular Sciences"],"ids":[13767,25198,14627],"codes":[0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp"],"datasets":[{"label":"","data":[1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":[20,20,20],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Springer Nature"],"ids":[202,8],"codes":[0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[2,1],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":[40,20],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Gavshina A. V. et al. The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti // Scientific Reports. 2021. Vol. 11. No. 1. 24314
GOST all authors (up to 50)
Copy
Gavshina A. V., Marynich N. K., Khrenova M. G., Solovyev I. D., Savitsky A. P. The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti // Scientific Reports. 2021. Vol. 11. No. 1. 24314
Cite this
RIS
Copy
TY - JOUR
DO - 10.1038/s41598-021-03634-9
UR - https://doi.org/10.1038%2Fs41598-021-03634-9
TI - The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti
T2 - Scientific Reports
AU - Gavshina, A V
AU - Marynich, N K
AU - Khrenova, M. G.
AU - Solovyev, I D
AU - Savitsky, A P
PY - 2021
DA - 2021/12/21 00:00:00
PB - Springer Nature
IS - 1
VL - 11
PMID - 34934103
SN - 2045-2322
ER -
Cite this
BibTex
Copy
@article{2021_Gavshina,
author = {A V Gavshina and N K Marynich and M. G. Khrenova and I D Solovyev and A P Savitsky},
title = {The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti},
journal = {Scientific Reports},
year = {2021},
volume = {11},
publisher = {Springer Nature},
month = {dec},
url = {https://doi.org/10.1038%2Fs41598-021-03634-9},
number = {1},
doi = {10.1038/s41598-021-03634-9}
}
Profiles