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том 11 издание 1 номер публикации 24314

The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti

Тип публикацииJournal Article
Дата публикации2021-12-21
scimago Q1
wos Q1
БС1
SJR0.874
CiteScore6.7
Impact factor3.9
ISSN20452322
Multidisciplinary
Краткое описание
Biphotochromic fluorescent protein SAASoti contains five cysteine residues in its sequence and a V127T point mutation transforms it to the monomeric form, mSAASoti. These cysteine residues are located far from the chromophore and might control its properties only allosterically. The influence of individual, double and triple cysteine substitutions of mSAASoti on fluorescent parameters and phototransformation reactions (irreversible green-to-red photoconversion and reversible photoswitching) is studied. A set of mSAASoti mutant forms (C21N, C117S, C71V, C105V, C175A, C21N/C71V, C21N/C175A, C21N/C71G/C175A) is obtained by site-directed mutagenesis. We demonstrate that the C21N variant exists in a monomeric form up to high concentrations, the C71V substitution accelerates photoconversion to the red form and the C105V variant has the maximum photoswitching rate. All C175A-containing variants demonstrate different photoswitching kinetics and decreased photostability during subsequent switching cycles compared with other considered systems. Classical molecular dynamic simulations reveal that the F177 side chain located in the vicinity of the chromophore is considerably more flexible in the mSAASoti compared with its C175A variant. This might be the explanation of the experimentally observed slowdown the thermal relaxation rate, i.e., trans–cis isomerization of the chromophore in mSAASoti upon C175A substitution.
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Gavshina A. V. et al. The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti // Scientific Reports. 2021. Vol. 11. No. 1. 24314
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Gavshina A. V., Marynich N. K., Khrenova M. G., Solovyev I. D., Savitsky A. P. The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti // Scientific Reports. 2021. Vol. 11. No. 1. 24314
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TY - JOUR
DO - 10.1038/s41598-021-03634-9
UR - https://doi.org/10.1038/s41598-021-03634-9
TI - The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti
T2 - Scientific Reports
AU - Gavshina, A V
AU - Marynich, N K
AU - Khrenova, M. G.
AU - Solovyev, I D
AU - Savitsky, A P
PY - 2021
DA - 2021/12/21
PB - Springer Nature
IS - 1
VL - 11
PMID - 34934103
SN - 2045-2322
ER -
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@article{2021_Gavshina,
author = {A V Gavshina and N K Marynich and M. G. Khrenova and I D Solovyev and A P Savitsky},
title = {The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti},
journal = {Scientific Reports},
year = {2021},
volume = {11},
publisher = {Springer Nature},
month = {dec},
url = {https://doi.org/10.1038/s41598-021-03634-9},
number = {1},
pages = {24314},
doi = {10.1038/s41598-021-03634-9}
}