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Scientific Reports, volume 12, issue 1, publication number 7862

First biphotochromic fluorescent protein moxSAASoti stabilized for oxidizing environment

Publication type: Journal Article
Publication date: 2022-05-12
Quartile SCImago
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Quartile WOS
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Impact factor: 4.6
ISSN: 20452322
Multidisciplinary
Abstract

Biphotochromic proteins simultaneously possess reversible photoswitching (on-to-off) and irreversible photoconversion (green-to-red). High photochemical reactivity of cysteine residues is one of the reasons for the development of “mox”-monomeric and oxidation resistant proteins. Based on site-saturated simultaneous two-point C105 and C117 mutagenesis, we chose C21N/C71G/C105G/C117T/C175A as the moxSAASoti variant. Since its on-to-off photoswitching rate is higher, off-to-on recovery is more complete and photoconversion rates are higher than those of mSAASoti. We analyzed the conformational behavior of the F177 side chain by classical MD simulations. The conformational flexibility of the F177 side chain is mainly responsible for the off-to-on conversion rate changes and can be further utilized as a measure of the conversion rate. Point mutations in mSAASoti mainly affect the pKa values of the red form and off-to-on switching. We demonstrate that the microscopic measure of the observed pKa value is the C–O bond length in the phenyl fragment of the neutral chromophore. According to molecular dynamics simulations with QM/MM potentials, larger C–O bond lengths are found for proteins with larger pKa. This feature can be utilized for prediction of the pKa values of red fluorescent proteins.

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Marynich N. K. et al. First biphotochromic fluorescent protein moxSAASoti stabilized for oxidizing environment // Scientific Reports. 2022. Vol. 12. No. 1. 7862
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Marynich N. K., Khrenova M. G., Gavshina A. V., Solovyev I. D., Savitsky A. P. First biphotochromic fluorescent protein moxSAASoti stabilized for oxidizing environment // Scientific Reports. 2022. Vol. 12. No. 1. 7862
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TY - JOUR
DO - 10.1038/s41598-022-11249-x
UR - https://doi.org/10.1038%2Fs41598-022-11249-x
TI - First biphotochromic fluorescent protein moxSAASoti stabilized for oxidizing environment
T2 - Scientific Reports
AU - Marynich, N K
AU - Khrenova, M. G.
AU - Gavshina, A V
AU - Solovyev, I D
AU - Savitsky, A P
PY - 2022
DA - 2022/05/12 00:00:00
PB - Springer Nature
IS - 1
VL - 12
PMID - 35551209
SN - 2045-2322
ER -
BibTex
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BibTex Copy
@article{2022_Marynich,
author = {N K Marynich and M. G. Khrenova and A V Gavshina and I D Solovyev and A P Savitsky},
title = {First biphotochromic fluorescent protein moxSAASoti stabilized for oxidizing environment},
journal = {Scientific Reports},
year = {2022},
volume = {12},
publisher = {Springer Nature},
month = {may},
url = {https://doi.org/10.1038%2Fs41598-022-11249-x},
number = {1},
doi = {10.1038/s41598-022-11249-x}
}
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