Open Access
Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon
Irina V Prokhorova
1
,
Ilya A. Osterman
1
,
Dmitry E Burakovsky
1, 2
,
Marina V. Serebryakova
1
,
Olga V Pobeguts
3
,
Ilya Altukhov
3, 4
,
Sergey Kovalchuk
3
,
Dmitry G Alexeev
3, 4
,
VADIM M. GOVORUN
3
,
Alexey A. Bogdanov
1
,
Petr V. Sergiev
1
,
Publication type: Journal Article
Publication date: 2013-11-18
scimago Q1
wos Q1
SJR: 0.874
CiteScore: 6.7
Impact factor: 3.9
ISSN: 20452322
PubMed ID:
24241179
Multidisciplinary
Abstract
Ribosomes contain a number of modifications in rRNA, the function of which is unclear. Here we show – using proteomic analysis and dual fluorescence reporter in vivo assays – that m2G966 and m5C967 in 16S rRNA of Escherichia coli ribosomes are necessary for correct attenuation of tryptophan (trp) operon. Expression of trp operon is upregulated in the strain where RsmD and RsmB methyltransferases were deleted, which results in the lack of m2G966 and m5C967 modifications. The upregulation requires the trpL attenuator, but is independent of the promotor of trp operon, ribosome binding site of the trpE gene, which follows trp attenuator and even Trp codons in the trpL sequence. Suboptimal translation initiation efficiency in the rsmB/rsmD knockout strain is likely to cause a delay in translation relative to transcription which causes misregulation of attenuation control of trp operon.
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Prokhorova I. V. et al. Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon // Scientific Reports. 2013. Vol. 3. No. 1. 3236
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Prokhorova I. V., Osterman I. A., Burakovsky D. E., Serebryakova M., Galyamina M. A., Pobeguts O. V., Altukhov I., Kovalchuk S., Alexeev D. G., GOVORUN V. M., Bogdanov A. A., Sergiev P. V., Dontsova O. A. Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon // Scientific Reports. 2013. Vol. 3. No. 1. 3236
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TY - JOUR
DO - 10.1038/srep03236
UR - https://www.nature.com/articles/srep03236
TI - Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon
T2 - Scientific Reports
AU - Prokhorova, Irina V
AU - Osterman, Ilya A.
AU - Burakovsky, Dmitry E
AU - Serebryakova, Marina V.
AU - Galyamina, Maria A
AU - Pobeguts, Olga V
AU - Altukhov, Ilya
AU - Kovalchuk, Sergey
AU - Alexeev, Dmitry G
AU - GOVORUN, VADIM M.
AU - Bogdanov, Alexey A.
AU - Sergiev, Petr V.
AU - Dontsova, Olga A.
PY - 2013
DA - 2013/11/18
PB - Springer Nature
IS - 1
VL - 3
PMID - 24241179
SN - 2045-2322
ER -
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BibTex (up to 50 authors)
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@article{2013_Prokhorova,
author = {Irina V Prokhorova and Ilya A. Osterman and Dmitry E Burakovsky and Marina V. Serebryakova and Maria A Galyamina and Olga V Pobeguts and Ilya Altukhov and Sergey Kovalchuk and Dmitry G Alexeev and VADIM M. GOVORUN and Alexey A. Bogdanov and Petr V. Sergiev and Olga A. Dontsova},
title = {Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon},
journal = {Scientific Reports},
year = {2013},
volume = {3},
publisher = {Springer Nature},
month = {nov},
url = {https://www.nature.com/articles/srep03236},
number = {1},
pages = {3236},
doi = {10.1038/srep03236}
}