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том 3 издание 1 номер публикации 3236

Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon

Тип публикацииJournal Article
Дата публикации2013-11-18
scimago Q1
wos Q1
БС1
SJR0.874
CiteScore6.7
Impact factor3.9
ISSN20452322
Multidisciplinary
Краткое описание
Ribosomes contain a number of modifications in rRNA, the function of which is unclear. Here we show – using proteomic analysis and dual fluorescence reporter in vivo assays – that m2G966 and m5C967 in 16S rRNA of Escherichia coli ribosomes are necessary for correct attenuation of tryptophan (trp) operon. Expression of trp operon is upregulated in the strain where RsmD and RsmB methyltransferases were deleted, which results in the lack of m2G966 and m5C967 modifications. The upregulation requires the trpL attenuator, but is independent of the promotor of trp operon, ribosome binding site of the trpE gene, which follows trp attenuator and even Trp codons in the trpL sequence. Suboptimal translation initiation efficiency in the rsmB/rsmD knockout strain is likely to cause a delay in translation relative to transcription which causes misregulation of attenuation control of trp operon.
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Prokhorova I. V. et al. Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon // Scientific Reports. 2013. Vol. 3. No. 1. 3236
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Prokhorova I. V., Osterman I. A., Burakovsky D. E., Serebryakova M., Galyamina M. A., Pobeguts O. V., Altukhov I., Kovalchuk S., Alexeev D. G., GOVORUN V. M., Bogdanov A. A., Sergiev P. V., Dontsova O. A. Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon // Scientific Reports. 2013. Vol. 3. No. 1. 3236
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TY - JOUR
DO - 10.1038/srep03236
UR - https://www.nature.com/articles/srep03236
TI - Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon
T2 - Scientific Reports
AU - Prokhorova, Irina V
AU - Osterman, Ilya A.
AU - Burakovsky, Dmitry E
AU - Serebryakova, Marina V.
AU - Galyamina, Maria A
AU - Pobeguts, Olga V
AU - Altukhov, Ilya
AU - Kovalchuk, Sergey
AU - Alexeev, Dmitry G
AU - GOVORUN, VADIM M.
AU - Bogdanov, Alexey A.
AU - Sergiev, Petr V.
AU - Dontsova, Olga A.
PY - 2013
DA - 2013/11/18
PB - Springer Nature
IS - 1
VL - 3
PMID - 24241179
SN - 2045-2322
ER -
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@article{2013_Prokhorova,
author = {Irina V Prokhorova and Ilya A. Osterman and Dmitry E Burakovsky and Marina V. Serebryakova and Maria A Galyamina and Olga V Pobeguts and Ilya Altukhov and Sergey Kovalchuk and Dmitry G Alexeev and VADIM M. GOVORUN and Alexey A. Bogdanov and Petr V. Sergiev and Olga A. Dontsova},
title = {Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon},
journal = {Scientific Reports},
year = {2013},
volume = {3},
publisher = {Springer Nature},
month = {nov},
url = {https://www.nature.com/articles/srep03236},
number = {1},
pages = {3236},
doi = {10.1038/srep03236}
}