The Analyst

, volume 146 , issue 12 , pages 4000-4009

An electrochemical biosensor for simultaneous detection of breast cancer clinically related microRNAs based on a gold nanoparticles/graphene quantum dots/graphene oxide film

Chammari Pothipor ^{1, 2, 3, 4, 5, 6, 7, 8}

Jaroon Jakmunee ^{1, 3, 4, 5, 6, 7, 8, 9}

Suwussa Bamrungsap ^{8, 10, 11, 12, 13}

Kontad Ounnunkad ^{1, 3, 4, 5, 6, 7, 8, 9, 14}

Hide authors affiliations Show authors affiliations: 14 affiliations

Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand |

The Graduate School, Chiang Mai University, Chiang Mai, Thailand |

Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand |

⁴

DEPARTMENT OF CHEMISTRY

⁵

Faculty of science

⁶

Chiang Mai University |

⁷

Chiang Mai 50200 |

⁸

THAILAND |

⁹

Research Center On Chemistry for Development of Health Promoting Products From Northern Resources, Chiang Mai University, Chiang Mai, Thailand |

¹⁰

National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand

National Science and Technology Development Agency

National Nanotechnology Center

¹¹

National Nanotechnology Center (NANOTEC) |

¹²

National Science and Technology Development Agency (NSTDA) |

¹³

Pathum Thani 12120 |

¹⁴

Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand |

Publication type: Journal Article

Publication date: 2021-04-26

Royal Society of Chemistry (RSC)

The Analyst

scimago Q2

wos Q2

SJR: 0.617

CiteScore: 7.0

Impact factor: 3.3

ISSN: 00032654, 13645528, 07417918

DOI: 10.1039/d1an00436k

Copy DOI

PubMed ID: 34013303

Biochemistry

Spectroscopy

Analytical Chemistry

Electrochemistry

Environmental Chemistry

Abstract

A label-free multiplexed electrochemical biosensor based on a gold nanoparticles/graphene quantum dots/graphene oxide (AuNPs/GQDs/GO) modified three-screen-printed carbon electrode (3SPCE) array is successfully constructed to detect miRNA-21, miRNA-155, and miRNA-210 biomarkers for the first time. Redox species (anthraquinone (AQ), methylene blue (MB), and polydopamine (PDA)) are used as redox indicators for anchoring capture miRNA probes, which hybridize with the complementary targets, miRNA-21, miRNA-155, and miRNA-210, respectively. After three target miRNAs are present, the square wave voltammetry (SWV) scan displays three well-separated peaks. Each peak indicates the presence of one miRNA, and its intensity quantitatively correlates with the concentration of the corresponding target analyte. This phenomenon results in the substantial decline of the SWV peak current of the redox probes. The developed AuNPs/GQDs/GO-based biosensor reveals excellent performance for simultaneous miRNA sensing. It offers a wide linear dynamic range from 0.001 to 1000 pM with ultrasensitive low detection limits of 0.04, 0.33, and 0.28 fM for the detection of miRNA-21, miRNA-155, and miRNA-210, respectively. It also presents high selectivity and applicability for the detection of miRNAs in human serum samples. This multiplex label-free miRNA biosensor has great potential for applications in breast cancer diagnosis.

Found

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GRABOWSKA Iwona

39 publications, 946 citations, 115 reviews

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Pothipor C. et al. An electrochemical biosensor for simultaneous detection of breast cancer clinically related microRNAs based on a gold nanoparticles/graphene quantum dots/graphene oxide film // The Analyst. 2021. Vol. 146. No. 12. pp. 4000-4009.

GOST all authors (up to 50) Copy

Pothipor C., Jakmunee J., Bamrungsap S., Ounnunkad K. An electrochemical biosensor for simultaneous detection of breast cancer clinically related microRNAs based on a gold nanoparticles/graphene quantum dots/graphene oxide film // The Analyst. 2021. Vol. 146. No. 12. pp. 4000-4009.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1039/d1an00436k

UR - https://xlink.rsc.org/?DOI=D1AN00436K

TI - An electrochemical biosensor for simultaneous detection of breast cancer clinically related microRNAs based on a gold nanoparticles/graphene quantum dots/graphene oxide film

T2 - The Analyst

AU - Pothipor, Chammari

AU - Jakmunee, Jaroon

AU - Bamrungsap, Suwussa

AU - Ounnunkad, Kontad

PY - 2021

DA - 2021/04/26

PB - Royal Society of Chemistry (RSC)

SP - 4000-4009

IS - 12

VL - 146

PMID - 34013303

SN - 0003-2654

SN - 1364-5528

SN - 0741-7918

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2021_Pothipor,

author = {Chammari Pothipor and Jaroon Jakmunee and Suwussa Bamrungsap and Kontad Ounnunkad},

title = {An electrochemical biosensor for simultaneous detection of breast cancer clinically related microRNAs based on a gold nanoparticles/graphene quantum dots/graphene oxide film},

journal = {The Analyst},

year = {2021},

volume = {146},

publisher = {Royal Society of Chemistry (RSC)},

month = {apr},

url = {https://xlink.rsc.org/?DOI=D1AN00436K},

number = {12},

pages = {4000--4009},

doi = {10.1039/d1an00436k}

}

MLA

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MLA Copy

Pothipor, Chammari, et al. “An electrochemical biosensor for simultaneous detection of breast cancer clinically related microRNAs based on a gold nanoparticles/graphene quantum dots/graphene oxide film.” The Analyst, vol. 146, no. 12, Apr. 2021, pp. 4000-4009. https://xlink.rsc.org/?DOI=D1AN00436K.

Publisher

Royal Society of Chemistry (RSC)

Journal

The Analyst

scimago Q2

wos Q2

SJR

0.617

CiteScore

7.0

Impact factor

3.3

ISSN

00032654 (Print)

13645528 (Electronic)

07417918 (Print)