Open Access
Open access
volume 4 issue 93 pages 51703-51710

Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

Nadezhda M. Zholobak 1
Ekaterina O Vitukova 2
Alla Yegorova 2
Yulia V Scripinets 2
Inna I Leonenko 2
Valeriy P Antonovich 2
1
 
Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv D0368, Ukraine
2
 
Bogatsky Physicochemical Institute, National Academy Of Sciences Of Ukraine, Odessa, Ukraine
Publication typeJournal Article
Publication date2014-09-22
scimago Q1
wos Q2
SJR0.777
CiteScore7.6
Impact factor4.6
ISSN20462069
General Chemistry
General Chemical Engineering
Abstract
A method for the direct monitoring of the interaction of reactive oxygen species (ROS) with cerium dioxide nanoparticles (CDN) in living cells is proposed based on the use of a complex of calcein and CDN. The CDN–calcein complex penetrates easily into a living cell and is decomposed readily by endogenous or exogenous ROS, releasing brightly fluorescent calcein, which can be observed using conventional fluorescent microscopy. This complex is less cytotoxic than individual cerium dioxide nanoparticles and provides effective protection from oxidative stress induced by the action of hydrogen peroxide, treatment with latex beads, or infection by the vesicular stomatitis virus.
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GOST |
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GOST Copy
Zholobak N. M. et al. Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells // RSC Advances. 2014. Vol. 4. No. 93. pp. 51703-51710.
GOST all authors (up to 50) Copy
Zholobak N. M., Shcherbakov A. B., Vitukova E. O., Yegorova A., Scripinets Y. V., Leonenko I. I., Baranchikov A. E., Antonovich V. P., Ivanov V. V. Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells // RSC Advances. 2014. Vol. 4. No. 93. pp. 51703-51710.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1039/c4ra08292c
UR - https://xlink.rsc.org/?DOI=C4RA08292C
TI - Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells
T2 - RSC Advances
AU - Zholobak, Nadezhda M.
AU - Shcherbakov, Alexander B.
AU - Vitukova, Ekaterina O
AU - Yegorova, Alla
AU - Scripinets, Yulia V
AU - Leonenko, Inna I
AU - Baranchikov, Alexander E
AU - Antonovich, Valeriy P
AU - Ivanov, V. V.
PY - 2014
DA - 2014/09/22
PB - Royal Society of Chemistry (RSC)
SP - 51703-51710
IS - 93
VL - 4
SN - 2046-2069
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2014_Zholobak,
author = {Nadezhda M. Zholobak and Alexander B. Shcherbakov and Ekaterina O Vitukova and Alla Yegorova and Yulia V Scripinets and Inna I Leonenko and Alexander E Baranchikov and Valeriy P Antonovich and V. V. Ivanov},
title = {Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells},
journal = {RSC Advances},
year = {2014},
volume = {4},
publisher = {Royal Society of Chemistry (RSC)},
month = {sep},
url = {https://xlink.rsc.org/?DOI=C4RA08292C},
number = {93},
pages = {51703--51710},
doi = {10.1039/c4ra08292c}
}
MLA
Cite this
MLA Copy
Zholobak, Nadezhda M., et al. “Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells.” RSC Advances, vol. 4, no. 93, Sep. 2014, pp. 51703-51710. https://xlink.rsc.org/?DOI=C4RA08292C.