Open Access
,
volume 101
,
issue 32
,
pages 11566-11570
De novo design of catalytic proteins
Publication type: Journal Article
Publication date: 2004-08-03
scimago Q1
wos Q1
SJR: 3.414
CiteScore: 16.5
Impact factor: 9.1
ISSN: 00278424, 10916490
PubMed ID:
15292507
Multidisciplinary
Abstract
The de novo design of catalytic proteins provides a stringent test of our understanding of enzyme function, while simultaneously laying the groundwork for the design of novel catalysts. Here we describe the design of an O 2 -dependent phenol oxidase whose structure, sequence, and activity are designed from first principles. The protein catalyzes the two-electron oxidation of 4-aminophenol ( k cat / K M = 1,500 M ·1 ·min ·1 ) to the corresponding quinone monoimine by using a diiron cofactor. The catalytic efficiency is sensitive to changes of the size of a methyl group in the protein, illustrating the specificity of the design.
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289
Total citations:
289
Citations from 2024:
14
(4.84%)
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GOST
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Kaplan J., DeGrado W. F. De novo design of catalytic proteins // Proceedings of the National Academy of Sciences of the United States of America. 2004. Vol. 101. No. 32. pp. 11566-11570.
GOST all authors (up to 50)
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Kaplan J., DeGrado W. F. De novo design of catalytic proteins // Proceedings of the National Academy of Sciences of the United States of America. 2004. Vol. 101. No. 32. pp. 11566-11570.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1073/pnas.0404387101
UR - https://doi.org/10.1073/pnas.0404387101
TI - De novo design of catalytic proteins
T2 - Proceedings of the National Academy of Sciences of the United States of America
AU - Kaplan, J.
AU - DeGrado, W. F.
PY - 2004
DA - 2004/08/03
PB - Proceedings of the National Academy of Sciences (PNAS)
SP - 11566-11570
IS - 32
VL - 101
PMID - 15292507
SN - 0027-8424
SN - 1091-6490
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2004_Kaplan,
author = {J. Kaplan and W. F. DeGrado},
title = {De novo design of catalytic proteins},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
year = {2004},
volume = {101},
publisher = {Proceedings of the National Academy of Sciences (PNAS)},
month = {aug},
url = {https://doi.org/10.1073/pnas.0404387101},
number = {32},
pages = {11566--11570},
doi = {10.1073/pnas.0404387101}
}
Cite this
MLA
Copy
Kaplan, J., and W. F. DeGrado. “De novo design of catalytic proteins.” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 32, Aug. 2004, pp. 11566-11570. https://doi.org/10.1073/pnas.0404387101.