Open Access
Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation
Тип публикации: Journal Article
Дата публикации: 2003-07-03
scimago Q1
wos Q2
БС1
SJR: 1.705
CiteScore: 7.6
Impact factor: 3.9
ISSN: 00219258, 1083351X
PubMed ID:
12730236
Biochemistry
Molecular Biology
Cell Biology
Краткое описание
A method for preparation of Escherichia coli ribosomes carrying lethal mutations in 23 S rRNA was developed. The method is based on the site-directed incorporation of a streptavidin binding tag into functionally neutral sites of the 23 S rRNA and subsequent affinity chromatography. It was tested with ribosomes mutated at the 23 S rRNA position 2655 (the elongation factor (EF)-G binding site). Ribosomes carrying the lethal G2655C mutation were purified and studied in vitro. It was found in particular that this mutation confers strong inhibition of the translocation process but only moderately affects GTPase activity and binding of EF-G.
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ГОСТ
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Leonov A. A. et al. Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation // Journal of Biological Chemistry. 2003. Vol. 278. No. 28. pp. 25664-25670.
ГОСТ со всеми авторами (до 50)
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Leonov A. A., Sergiev P. V., Bogdanov A. A., BRIMACOMBE R., Dontsova O. A. Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation // Journal of Biological Chemistry. 2003. Vol. 278. No. 28. pp. 25664-25670.
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TY - JOUR
DO - 10.1074/jbc.M302873200
UR - https://linkinghub.elsevier.com/retrieve/pii/S0021925819750832
TI - Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation
T2 - Journal of Biological Chemistry
AU - Leonov, Andrei A.
AU - Sergiev, Petr V.
AU - Bogdanov, Alexey A.
AU - BRIMACOMBE, RICHARD
AU - Dontsova, Olga A.
PY - 2003
DA - 2003/07/03
PB - Elsevier
SP - 25664-25670
IS - 28
VL - 278
PMID - 12730236
SN - 0021-9258
SN - 1083-351X
ER -
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BibTex (до 50 авторов)
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@article{2003_Leonov,
author = {Andrei A. Leonov and Petr V. Sergiev and Alexey A. Bogdanov and RICHARD BRIMACOMBE and Olga A. Dontsova},
title = {Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation},
journal = {Journal of Biological Chemistry},
year = {2003},
volume = {278},
publisher = {Elsevier},
month = {jul},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0021925819750832},
number = {28},
pages = {25664--25670},
doi = {10.1074/jbc.M302873200}
}
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MLA
Скопировать
Leonov, Andrei A., et al. “Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation.” Journal of Biological Chemistry, vol. 278, no. 28, Jul. 2003, pp. 25664-25670. https://linkinghub.elsevier.com/retrieve/pii/S0021925819750832.