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Journal of Biological Chemistry, volume 278, issue 28, pages 25664-25670

Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation

Leonov Andrei A. 1
BRIMACOMBE RICHARD 2, 3
Bogdanov Alexey A. 2
2
 
Department of Chemistry, Moscow State University, 119899 Moscow, Russia, 14195 Berlin, Germany
3
 
Max Planck Institut fur Molekulare Genetik, 14195 Berlin, Germany
Publication typeJournal Article
Publication date2003-07-03
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor4.8
ISSN00219258, 1083351X
Biochemistry
Molecular Biology
Cell Biology
Abstract
A method for preparation of Escherichia coli ribosomes carrying lethal mutations in 23 S rRNA was developed. The method is based on the site-directed incorporation of a streptavidin binding tag into functionally neutral sites of the 23 S rRNA and subsequent affinity chromatography. It was tested with ribosomes mutated at the 23 S rRNA position 2655 (the elongation factor (EF)-G binding site). Ribosomes carrying the lethal G2655C mutation were purified and studied in vitro. It was found in particular that this mutation confers strong inhibition of the translocation process but only moderately affects GTPase activity and binding of EF-G.

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GOST Copy
Leonov A. A. et al. Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation // Journal of Biological Chemistry. 2003. Vol. 278. No. 28. pp. 25664-25670.
GOST all authors (up to 50) Copy
Leonov A. A., Bogdanov A. A., BRIMACOMBE R., Dontsova O. A., Sergiev P. V., Bogdanov A. A. Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation // Journal of Biological Chemistry. 2003. Vol. 278. No. 28. pp. 25664-25670.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1074/jbc.M302873200
UR - https://doi.org/10.1074%2Fjbc.M302873200
TI - Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation
T2 - Journal of Biological Chemistry
AU - Leonov, Andrei A.
AU - Bogdanov, Alexey A.
AU - BRIMACOMBE, RICHARD
AU - Dontsova, Olga A.
AU - Sergiev, Petr V.
AU - Bogdanov, Alexey A.
PY - 2003
DA - 2003/07/03 00:00:00
PB - American Society for Biochemistry and Molecular Biology
SP - 25664-25670
IS - 28
VL - 278
SN - 0021-9258
SN - 1083-351X
ER -
BibTex |
Cite this
BibTex Copy
@article{2003_Leonov
author = {Andrei A. Leonov and Alexey A. Bogdanov and RICHARD BRIMACOMBE and Olga A. Dontsova and Petr V. Sergiev and Alexey A. Bogdanov},
title = {Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation},
journal = {Journal of Biological Chemistry},
year = {2003},
volume = {278},
publisher = {American Society for Biochemistry and Molecular Biology},
month = {jul},
url = {https://doi.org/10.1074%2Fjbc.M302873200},
number = {28},
pages = {25664--25670},
doi = {10.1074/jbc.M302873200}
}
MLA
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MLA Copy
Leonov, Andrei A., et al. “Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation.” Journal of Biological Chemistry, vol. 278, no. 28, Jul. 2003, pp. 25664-25670. https://doi.org/10.1074%2Fjbc.M302873200.
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