Open Access
Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation
2
Department of Chemistry, Moscow State University, 119899 Moscow, Russia, 14195 Berlin, Germany
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Publication type: Journal Article
Publication date: 2003-07-03
scimago Q1
wos Q2
SJR: 1.705
CiteScore: 7.6
Impact factor: 3.9
ISSN: 00219258, 1083351X
PubMed ID:
12730236
Biochemistry
Molecular Biology
Cell Biology
Abstract
A method for preparation of Escherichia coli ribosomes carrying lethal mutations in 23 S rRNA was developed. The method is based on the site-directed incorporation of a streptavidin binding tag into functionally neutral sites of the 23 S rRNA and subsequent affinity chromatography. It was tested with ribosomes mutated at the 23 S rRNA position 2655 (the elongation factor (EF)-G binding site). Ribosomes carrying the lethal G2655C mutation were purified and studied in vitro. It was found in particular that this mutation confers strong inhibition of the translocation process but only moderately affects GTPase activity and binding of EF-G.
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51
Total citations:
51
Citations from 2024:
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(3%)
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MLA
Cite this
GOST
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Leonov A. A. et al. Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation // Journal of Biological Chemistry. 2003. Vol. 278. No. 28. pp. 25664-25670.
GOST all authors (up to 50)
Copy
Leonov A. A., Sergiev P. V., Bogdanov A. A., BRIMACOMBE R., Dontsova O. A. Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation // Journal of Biological Chemistry. 2003. Vol. 278. No. 28. pp. 25664-25670.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1074/jbc.M302873200
UR - https://linkinghub.elsevier.com/retrieve/pii/S0021925819750832
TI - Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation
T2 - Journal of Biological Chemistry
AU - Leonov, Andrei A.
AU - Sergiev, Petr V.
AU - Bogdanov, Alexey A.
AU - BRIMACOMBE, RICHARD
AU - Dontsova, Olga A.
PY - 2003
DA - 2003/07/03
PB - Elsevier
SP - 25664-25670
IS - 28
VL - 278
PMID - 12730236
SN - 0021-9258
SN - 1083-351X
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2003_Leonov,
author = {Andrei A. Leonov and Petr V. Sergiev and Alexey A. Bogdanov and RICHARD BRIMACOMBE and Olga A. Dontsova},
title = {Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation},
journal = {Journal of Biological Chemistry},
year = {2003},
volume = {278},
publisher = {Elsevier},
month = {jul},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0021925819750832},
number = {28},
pages = {25664--25670},
doi = {10.1074/jbc.M302873200}
}
Cite this
MLA
Copy
Leonov, Andrei A., et al. “Affinity purification of ribosomes with a lethal G2655C mutation in 23 s rRNA that affects the translocation.” Journal of Biological Chemistry, vol. 278, no. 28, Jul. 2003, pp. 25664-25670. https://linkinghub.elsevier.com/retrieve/pii/S0021925819750832.
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