Open Access
Journal of Biological Chemistry, volume 280, issue 36, pages 31882-31889
Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G
Sergiev Petr V.
1
,
Lesnyak Dmitry V
2
,
Burakovsky Dmitry E
1
,
Kiparisov Sergey V
1
,
Leonov Andrei A.
1
,
Bogdanov Alexey A.
1
,
BRIMACOMBE RICHARD
3
,
3
Max-Planck-Institut fuer Molekulare Genetik, Berlin, D-14195, Germany
|
Publication type: Journal Article
Publication date: 2005-09-01
Journal:
Journal of Biological Chemistry
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 4.8
ISSN: 00219258, 1083351X
Biochemistry
Molecular Biology
Cell Biology
Abstract
Translocation catalyzed by elongation factor G occurs after the peptidyltransferase reaction on the large ribosomal subunit. Deacylated tRNA in the P-site stimulates multiple turnover GTPase activity of EF-G. We suggest that the allosteric signal from the peptidyltransferase center that activates EF-G may involve the alteration in the conformation of elongation factor binding center of the ribosome. The latter consists of the moveable GTPase-associated center and the sarcin-ricin loop that keeps its position on the ribosome during translation elongation. The position of the GTPase-associated center was altered by mutagenesis. An insertion of additional base pair at positions C1030/G1124 was lethal and affected function of EF-G, but not that of EF-Tu. Structure probing revealed a putative allosteric signal pathway connecting the P-site with the binding site of the elongation factors. The results are consistent with the different structural requirements for EF-G and EF-Tu function, where the integrity of the path between the peptidyltransferase center and both GTPase-associated center and sarcin-ricin loop is important for EF-G binding.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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GOST
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Sergiev P. V. et al. Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G // Journal of Biological Chemistry. 2005. Vol. 280. No. 36. pp. 31882-31889.
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Sergiev P. V., Lesnyak D. V., Burakovsky D. E., Kiparisov S. V., Leonov A. A., Bogdanov A. A., BRIMACOMBE R., Dontsova O. A. Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G // Journal of Biological Chemistry. 2005. Vol. 280. No. 36. pp. 31882-31889.
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RIS
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TY - JOUR
DO - 10.1074/jbc.M505670200
UR - https://doi.org/10.1074%2Fjbc.M505670200
TI - Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G
T2 - Journal of Biological Chemistry
AU - Sergiev, Petr V.
AU - Lesnyak, Dmitry V
AU - Burakovsky, Dmitry E
AU - Kiparisov, Sergey V
AU - Leonov, Andrei A.
AU - Bogdanov, Alexey A.
AU - BRIMACOMBE, RICHARD
AU - Dontsova, Olga A.
PY - 2005
DA - 2005/09/01 00:00:00
PB - American Society for Biochemistry and Molecular Biology
SP - 31882-31889
IS - 36
VL - 280
SN - 0021-9258
SN - 1083-351X
ER -
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BibTex
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@article{2005_Sergiev,
author = {Petr V. Sergiev and Dmitry V Lesnyak and Dmitry E Burakovsky and Sergey V Kiparisov and Andrei A. Leonov and Alexey A. Bogdanov and RICHARD BRIMACOMBE and Olga A. Dontsova},
title = {Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G},
journal = {Journal of Biological Chemistry},
year = {2005},
volume = {280},
publisher = {American Society for Biochemistry and Molecular Biology},
month = {sep},
url = {https://doi.org/10.1074%2Fjbc.M505670200},
number = {36},
pages = {31882--31889},
doi = {10.1074/jbc.M505670200}
}
Cite this
MLA
Copy
Sergiev, Petr V., et al. “Alteration in location of a conserved GTPase-associated center of the ribosome induced by mutagenesis influences the structure of peptidyltransferase center and activity of elongation factor G.” Journal of Biological Chemistry, vol. 280, no. 36, Sep. 2005, pp. 31882-31889. https://doi.org/10.1074%2Fjbc.M505670200.
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