Cyclodextrin Bonded Phase for Liquid Chromatographic Separation and Analysis of Some Oral Contraceptives
Publication type: Journal Article
Publication date: 1991-12-01
scimago Q3
wos Q3
SJR: 0.334
CiteScore: 3.6
Impact factor: 1.8
ISSN: 00032719, 1532236X
Biochemistry
Spectroscopy
Clinical Biochemistry
Analytical Chemistry
Electrochemistry
Biochemistry (medical)
Abstract
A specific and sensitive analytical HPLC procedure was described for quantitative determination of ethinylestradiol and norethisterone acetate (Anovlar 1) and ethinylestradiol and norgestrel (Primovlar) in tablet formulation. These steroids were extracted from the tablets with methanol. The steroids were then determined with high performance liquid Chromatograph-Cyclobond 1 column using mobile phase phosphate buffer pH 7.0: methanol (60:40), flow rate 0.5 ml min−1 and the detection was effected spectrophotometrically at 280 nm, using variable wavelength UV detector. There was > 99.3% recovery from synthetic mixtures and the coefficient of variation was < 2.0% for the formulations investigated. The method is highly quantitative and reproducible.
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Ahmed A. H. N., El-Gizawy S. M., Omar N. M. Cyclodextrin Bonded Phase for Liquid Chromatographic Separation and Analysis of Some Oral Contraceptives // Analytical Letters. 1991. Vol. 24. No. 12. pp. 2207-2216.
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Ahmed A. H. N., El-Gizawy S. M., Omar N. M. Cyclodextrin Bonded Phase for Liquid Chromatographic Separation and Analysis of Some Oral Contraceptives // Analytical Letters. 1991. Vol. 24. No. 12. pp. 2207-2216.
Cite this
RIS
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TY - JOUR
DO - 10.1080/00032719108053045
UR - https://doi.org/10.1080/00032719108053045
TI - Cyclodextrin Bonded Phase for Liquid Chromatographic Separation and Analysis of Some Oral Contraceptives
T2 - Analytical Letters
AU - Ahmed, Abdel Hamed N
AU - El-Gizawy, Samia M.
AU - Omar, Nabil M
PY - 1991
DA - 1991/12/01
PB - Taylor & Francis
SP - 2207-2216
IS - 12
VL - 24
SN - 0003-2719
SN - 1532-236X
ER -
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BibTex (up to 50 authors)
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@article{1991_Ahmed,
author = {Abdel Hamed N Ahmed and Samia M. El-Gizawy and Nabil M Omar},
title = {Cyclodextrin Bonded Phase for Liquid Chromatographic Separation and Analysis of Some Oral Contraceptives},
journal = {Analytical Letters},
year = {1991},
volume = {24},
publisher = {Taylor & Francis},
month = {dec},
url = {https://doi.org/10.1080/00032719108053045},
number = {12},
pages = {2207--2216},
doi = {10.1080/00032719108053045}
}
Cite this
MLA
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Ahmed, Abdel Hamed N., et al. “Cyclodextrin Bonded Phase for Liquid Chromatographic Separation and Analysis of Some Oral Contraceptives.” Analytical Letters, vol. 24, no. 12, Dec. 1991, pp. 2207-2216. https://doi.org/10.1080/00032719108053045.