Molecular Biology of the Cell, volume 13, issue 2, pages 425-434

Nuclear Pore Complex Is Able to Transport Macromolecules with Diameters of ∼39 nm

Publication typeJournal Article
Publication date2002-02-01
scimago Q1
SJR1.566
CiteScore6.0
Impact factor3.1
ISSN10591524, 19394586
Molecular Biology
Cell Biology
Abstract

Bidirectional transport of macromolecules between the nucleus and the cytoplasm occurs through the nuclear pore complexes (NPCs) by a signal-mediated mechanism that is directed by targeting signals (NLSs) residing on the transported molecules or “cargoes.” Nuclear transport starts after interaction of the targeting signal with soluble cellular receptors. After the formation of the cargo-receptor complex in the cytosol, this complex crosses the NPC. Herein, we use gold particles of various sizes coated with cargo-receptor complexes to determine precisely how large macromolecules crossing the NPC by the signal-mediated transport mechanism could be. We found that cargo-receptor-gold complexes with diameter close to 39 nm could be translocated by the NPC. This implies that macromolecules much larger than the assumed functional NPC diameter of 26 nm can be transported into the karyoplasm. The physiological relevance of this finding was supported by the observation that intact nucleocapsids of human hepatitis B virus with diameters of 32 and 36 nm are able to cross the nuclear pore without disassembly.

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