Open Access
Translation at first sight: The influence of leading codons
Ilya A Osterman
1, 2
,
Zoe Chervontseva
1, 3
,
Ekaterina S Komarova
1, 2
,
Timofei S. Zatsepin
1, 2
,
MICHAEL J. GELFAND
1, 3
,
Zoe S. Chervontseva
1, 3
,
Sergey A Evfratov
2
,
Alena V Sorokina
1
,
Vladimir A. Rodin
2
,
Maria Rubtsova
1, 2
,
Alexey Bogdanov
2
,
Mikhail S. Gelfand
1, 3
,
Olga A. Dontsova
1, 2, 5
,
Petr V. Sergiev
1, 2
Publication type: Journal Article
Publication date: 2020-05-19
scimago Q1
wos Q1
SJR: 7.776
CiteScore: 31.7
Impact factor: 13.1
ISSN: 03051048, 13624962
PubMed ID:
32427319
Genetics
Abstract
First triplets of mRNA coding region affect the yield of translation. We have applied the flowseq method to analyze >30 000 variants of the codons 2–11 of the fluorescent protein reporter to identify factors affecting the protein synthesis. While the negative influence of mRNA secondary structure on translation has been confirmed, a positive role of rare codons at the beginning of a coding sequence for gene expression has not been observed. The identity of triplets proximal to the start codon contributes more to the protein yield then more distant ones. Additional in-frame start codons enhance translation, while Shine–Dalgarno-like motifs downstream the initiation codon are inhibitory. The metabolic cost of amino acids affects the yield of protein in the poor medium. The most efficient translation was observed for variants with features resembling those of native Escherichia coli genes.
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Metrics
35
Total citations:
35
Citations from 2024:
11
(31%)
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MLA
Cite this
GOST
Copy
Osterman I. A. et al. Translation at first sight: The influence of leading codons // Nucleic Acids Research. 2020. Vol. 48. No. 12. pp. 6931-6942.
GOST all authors (up to 50)
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Osterman I. A., Chervontseva Z., Komarova E. S., Zatsepin T. S., Kabilov M. R., GELFAND M. J., Chervontseva Z. S., Evfratov S. A., Sorokina A. V., Rodin V. A., Rubtsova M., Bogdanov A., Gelfand M. S., Dontsova O. A., Sergiev P. V. Translation at first sight: The influence of leading codons // Nucleic Acids Research. 2020. Vol. 48. No. 12. pp. 6931-6942.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1093/nar/gkaa430
UR - https://academic.oup.com/nar/article/48/12/6931/5840581
TI - Translation at first sight: The influence of leading codons
T2 - Nucleic Acids Research
AU - Osterman, Ilya A
AU - Chervontseva, Zoe
AU - Komarova, Ekaterina S
AU - Zatsepin, Timofei S.
AU - Kabilov, Marsel R.
AU - GELFAND, MICHAEL J.
AU - Chervontseva, Zoe S.
AU - Evfratov, Sergey A
AU - Sorokina, Alena V
AU - Rodin, Vladimir A.
AU - Rubtsova, Maria
AU - Bogdanov, Alexey
AU - Gelfand, Mikhail S.
AU - Dontsova, Olga A.
AU - Sergiev, Petr V.
PY - 2020
DA - 2020/05/19
PB - Oxford University Press
SP - 6931-6942
IS - 12
VL - 48
PMID - 32427319
SN - 0305-1048
SN - 1362-4962
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2020_Osterman,
author = {Ilya A Osterman and Zoe Chervontseva and Ekaterina S Komarova and Timofei S. Zatsepin and Marsel R. Kabilov and MICHAEL J. GELFAND and Zoe S. Chervontseva and Sergey A Evfratov and Alena V Sorokina and Vladimir A. Rodin and Maria Rubtsova and Alexey Bogdanov and Mikhail S. Gelfand and Olga A. Dontsova and Petr V. Sergiev},
title = {Translation at first sight: The influence of leading codons},
journal = {Nucleic Acids Research},
year = {2020},
volume = {48},
publisher = {Oxford University Press},
month = {may},
url = {https://academic.oup.com/nar/article/48/12/6931/5840581},
number = {12},
pages = {6931--6942},
doi = {10.1093/nar/gkaa430}
}
Cite this
MLA
Copy
Osterman, Ilya A., et al. “Translation at first sight: The influence of leading codons.” Nucleic Acids Research, vol. 48, no. 12, May. 2020, pp. 6931-6942. https://academic.oup.com/nar/article/48/12/6931/5840581.