Translation at first sight: The influence of leading codons
First triplets of mRNA coding region affect the yield of translation. We have applied the flowseq method to analyze >30 000 variants of the codons 2–11 of the fluorescent protein reporter to identify factors affecting the protein synthesis. While the negative influence of mRNA secondary structure on translation has been confirmed, a positive role of rare codons at the beginning of a coding sequence for gene expression has not been observed. The identity of triplets proximal to the start codon contributes more to the protein yield then more distant ones. Additional in-frame start codons enhance translation, while Shine–Dalgarno-like motifs downstream the initiation codon are inhibitory. The metabolic cost of amino acids affects the yield of protein in the poor medium. The most efficient translation was observed for variants with features resembling those of native Escherichia coli genes.
Citations by journals
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
3 publications, 12.5%
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Nucleic Acids Research
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3 publications, 12.5%
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1 publication, 4.17%
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Frontiers in Microbiology
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1 publication, 4.17%
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Scientific Reports
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Scientific Reports
1 publication, 4.17%
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Synthetic and Systems Biotechnology
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1 publication, 4.17%
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Microbial Biotechnology
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Microbial Biotechnology
1 publication, 4.17%
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Biotechnology Journal
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Biotechnology Journal
1 publication, 4.17%
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ACS Synthetic Biology
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ACS Synthetic Biology
1 publication, 4.17%
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Bioinformatics
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Bioinformatics
1 publication, 4.17%
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Genome Biology and Evolution
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Genome Biology and Evolution
1 publication, 4.17%
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EMBO Journal
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EMBO Journal
1 publication, 4.17%
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Microbiology and Molecular Biology Reviews
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Microbiology and Molecular Biology Reviews
1 publication, 4.17%
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Acta Naturae
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Acta Naturae, 1, 4.17%
Acta Naturae
1 publication, 4.17%
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Nature Communications
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Nature Communications
1 publication, 4.17%
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Microbial Cell Factories
1 publication, 4.17%
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Citations by publishers
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5
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Oxford University Press
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Oxford University Press
5 publications, 20.83%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 12.5%
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Springer Nature
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Springer Nature
3 publications, 12.5%
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Wiley
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Wiley
2 publications, 8.33%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 4.17%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 4.17%
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KeAi Communications Co.
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KeAi Communications Co.
1 publication, 4.17%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
1 publication, 4.17%
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EMBO press
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EMBO press
1 publication, 4.17%
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American Society for Microbiology
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American Society for Microbiology
1 publication, 4.17%
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Acta Naturae Ltd
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Acta Naturae Ltd, 1, 4.17%
Acta Naturae Ltd
1 publication, 4.17%
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