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Nucleic Acids Research, volume 48, issue 12, pages 6931-6942

Translation at first sight: The influence of leading codons

Dontsova Olga A. 1, 2, 3
Sergiev Petr V. 1, 2
Osterman Ilya A 1, 2
Evfratov Sergey A 1
Sorokina Alena V 2
Rodin Vladimir A. 1
Rubtsova Maria 1, 2
Chervontseva Zoe 2, 5
GELFAND MICHAEL J. 2, 5
Publication typeJournal Article
Publication date2020-05-19
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor14.9
ISSN03051048, 13624962
PubMed ID:  32427319
Genetics
Abstract

First triplets of mRNA coding region affect the yield of translation. We have applied the flowseq method to analyze >30 000 variants of the codons 2–11 of the fluorescent protein reporter to identify factors affecting the protein synthesis. While the negative influence of mRNA secondary structure on translation has been confirmed, a positive role of rare codons at the beginning of a coding sequence for gene expression has not been observed. The identity of triplets proximal to the start codon contributes more to the protein yield then more distant ones. Additional in-frame start codons enhance translation, while Shine–Dalgarno-like motifs downstream the initiation codon are inhibitory. The metabolic cost of amino acids affects the yield of protein in the poor medium. The most efficient translation was observed for variants with features resembling those of native Escherichia coli genes.

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GOST |
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GOST Copy
Evfratov S. A. et al. Translation at first sight: The influence of leading codons // Nucleic Acids Research. 2020. Vol. 48. No. 12. pp. 6931-6942.
GOST all authors (up to 50) Copy
Evfratov S. A., Sorokina A. V., Rodin V. A., Dontsova O. A., Sergiev P. V., Rubtsova M., Bogdanov A., Osterman I. A., Chervontseva Z., Komarova E. S., Zatsepin T. S., Kabilov M. R., GELFAND M. J. Translation at first sight: The influence of leading codons // Nucleic Acids Research. 2020. Vol. 48. No. 12. pp. 6931-6942.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1093/nar/gkaa430
UR - https://doi.org/10.1093%2Fnar%2Fgkaa430
TI - Translation at first sight: The influence of leading codons
T2 - Nucleic Acids Research
AU - Evfratov, Sergey A
AU - Sorokina, Alena V
AU - Rodin, Vladimir A.
AU - Dontsova, Olga A.
AU - Sergiev, Petr V.
AU - Rubtsova, Maria
AU - Bogdanov, Alexey
AU - Osterman, Ilya A
AU - Chervontseva, Zoe
AU - Komarova, Ekaterina S
AU - Zatsepin, Timofei S.
AU - Kabilov, Marsel R.
AU - GELFAND, MICHAEL J.
PY - 2020
DA - 2020/05/19 00:00:00
PB - Oxford University Press
SP - 6931-6942
IS - 12
VL - 48
PMID - 32427319
SN - 0305-1048
SN - 1362-4962
ER -
BibTex |
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BibTex Copy
@article{2020_Evfratov
author = {Sergey A Evfratov and Alena V Sorokina and Vladimir A. Rodin and Olga A. Dontsova and Petr V. Sergiev and Maria Rubtsova and Alexey Bogdanov and Ilya A Osterman and Zoe Chervontseva and Ekaterina S Komarova and Timofei S. Zatsepin and Marsel R. Kabilov and MICHAEL J. GELFAND},
title = {Translation at first sight: The influence of leading codons},
journal = {Nucleic Acids Research},
year = {2020},
volume = {48},
publisher = {Oxford University Press},
month = {may},
url = {https://doi.org/10.1093%2Fnar%2Fgkaa430},
number = {12},
pages = {6931--6942},
doi = {10.1093/nar/gkaa430}
}
MLA
Cite this
MLA Copy
Evfratov, Sergey A., et al. “Translation at first sight: The influence of leading codons.” Nucleic Acids Research, vol. 48, no. 12, May. 2020, pp. 6931-6942. https://doi.org/10.1093%2Fnar%2Fgkaa430.
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