Open Access
Open access
volume 48 issue 14 pages 8022-8034

METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue

Ivan G. Laptev 1, 2, 3
Ivan Laptev 1, 2, 3
Ekaterina Shvetsova 4
Sergey Levitskii 5
Marina Serebryakova 1, 3
Maria Rubtsova 1, 2
Victor Zgoda 6
Alexey Bogdanov 2, 3
Piotr Kamenski 5
Petr V. Sergiev 1, 2, 3, 7
Olga Dontsova 1, 2, 3, 8
Publication typeJournal Article
Publication date2020-06-23
scimago Q1
wos Q1
SJR7.776
CiteScore31.7
Impact factor13.1
ISSN03051048, 13624962
PubMed ID:  32573735
Genetics
Abstract

Mammalian mitochondrial ribosomes contain a set of modified nucleotides, which is distinct from that of the cytosolic ribosomes. Nucleotide m4C840 of the murine mitochondrial 12S rRNA is equivalent to the dimethylated m4Cm1402 residue of Escherichia coli 16S rRNA. Here we demonstrate that mouse METTL15 protein is responsible for the formation of m4C residue of the 12S rRNA. Inactivation of Mettl15 gene in murine cell line perturbs the composition of mitochondrial protein biosynthesis machinery. Identification of METTL15 interaction partners revealed that the likely substrate for this RNA methyltransferase is an assembly intermediate of the mitochondrial small ribosomal subunit containing an assembly factor RBFA.

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Laptev I. G. et al. METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue // Nucleic Acids Research. 2020. Vol. 48. No. 14. pp. 8022-8034.
GOST all authors (up to 50) Copy
Laptev I. G., Laptev I., Shvetsova E., Levitskii S., Serebryakova M. V., Serebryakova M., Rubtsova M., Zgoda V., Bogdanov A., Kamenski P., Sergiev P. V., Dontsova O. METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue // Nucleic Acids Research. 2020. Vol. 48. No. 14. pp. 8022-8034.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1093/nar/gkaa522
UR - https://academic.oup.com/nar/article/48/14/8022/5861404
TI - METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue
T2 - Nucleic Acids Research
AU - Laptev, Ivan G.
AU - Laptev, Ivan
AU - Shvetsova, Ekaterina
AU - Levitskii, Sergey
AU - Serebryakova, M. V.
AU - Serebryakova, Marina
AU - Rubtsova, Maria
AU - Zgoda, Victor
AU - Bogdanov, Alexey
AU - Kamenski, Piotr
AU - Sergiev, Petr V.
AU - Dontsova, Olga
PY - 2020
DA - 2020/06/23
PB - Oxford University Press
SP - 8022-8034
IS - 14
VL - 48
PMID - 32573735
SN - 0305-1048
SN - 1362-4962
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2020_Laptev,
author = {Ivan G. Laptev and Ivan Laptev and Ekaterina Shvetsova and Sergey Levitskii and M. V. Serebryakova and Marina Serebryakova and Maria Rubtsova and Victor Zgoda and Alexey Bogdanov and Piotr Kamenski and Petr V. Sergiev and Olga Dontsova},
title = {METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue},
journal = {Nucleic Acids Research},
year = {2020},
volume = {48},
publisher = {Oxford University Press},
month = {jun},
url = {https://academic.oup.com/nar/article/48/14/8022/5861404},
number = {14},
pages = {8022--8034},
doi = {10.1093/nar/gkaa522}
}
MLA
Cite this
MLA Copy
Laptev, Ivan G., et al. “METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue.” Nucleic Acids Research, vol. 48, no. 14, Jun. 2020, pp. 8022-8034. https://academic.oup.com/nar/article/48/14/8022/5861404.