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Nucleic Acids Research, volume 48, issue 19, pages 10802-10819

Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor

Pletnev Philipp 1, 2
Pupov Danil 3
Pshanichnaya Lizaveta 2
Esyunina Daria 3
Petushkov Ivan 3
Nesterchuk Mikhail 4
Osterman Ilya A 2, 4
Rubtsova Maria 2, 4
Ravin N. V. 5
Sergiev Petr V. 2, 4, 6, 7
Dontsova Olga 1, 2, 4, 6
Publication typeJournal Article
Publication date2020-09-30
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor14.9
ISSN03051048, 13624962
PubMed ID:  32997144
Genetics
Abstract

In bacteria, rapid adaptation to changing environmental conditions depends on the interplay between housekeeping and alternative σ factors, responsible for transcription of specific regulons by RNA polymerase (RNAP). In comparison with alternative σ factors, primary σs contain poorly conserved region 1.1, whose functions in transcription are only partially understood. We found that a single mutation in region 1.1 in Escherichia coli σ70 rewires transcription regulation during cell growth resulting in profound phenotypic changes. Despite its destabilizing effect on promoter complexes, this mutation increases the activity of rRNA promoters and also decreases RNAP sensitivity to the major regulator of stringent response DksA. Using total RNA sequencing combined with single-cell analysis of gene expression we showed that changes in region 1.1 disrupt the balance between the "greed" and "fear" strategies thus making the cells more susceptible to environmental threats and antibiotics. Our results reveal an unexpected role of σ region 1.1 in growth-dependent transcription regulation and suggest that changes in this region may facilitate rapid switching of RNAP properties in evolving bacterial populations.

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Pletnev P. et al. Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor // Nucleic Acids Research. 2020. Vol. 48. No. 19. pp. 10802-10819.
GOST all authors (up to 50) Copy
Pletnev P., Pupov D., Pshanichnaya L., Esyunina D., Petushkov I., Nesterchuk M., Osterman I. A., Rubtsova M., Mardanov A. V., Ravin N. V., Sergiev P. V., Kulbachinskiy A., Dontsova O. Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor // Nucleic Acids Research. 2020. Vol. 48. No. 19. pp. 10802-10819.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1093/nar/gkaa798
UR - https://doi.org/10.1093%2Fnar%2Fgkaa798
TI - Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor
T2 - Nucleic Acids Research
AU - Pletnev, Philipp
AU - Pshanichnaya, Lizaveta
AU - Esyunina, Daria
AU - Petushkov, Ivan
AU - Nesterchuk, Mikhail
AU - Sergiev, Petr V.
AU - Kulbachinskiy, Andrey
AU - Dontsova, Olga
AU - Rubtsova, Maria
AU - Pupov, Danil
AU - Osterman, Ilya A
AU - Mardanov, A. V.
AU - Ravin, N. V.
PY - 2020
DA - 2020/09/30 00:00:00
PB - Oxford University Press
SP - 10802-10819
IS - 19
VL - 48
PMID - 32997144
SN - 0305-1048
SN - 1362-4962
ER -
BibTex |
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@article{2020_Pletnev
author = {Philipp Pletnev and Lizaveta Pshanichnaya and Daria Esyunina and Ivan Petushkov and Mikhail Nesterchuk and Petr V. Sergiev and Andrey Kulbachinskiy and Olga Dontsova and Maria Rubtsova and Danil Pupov and Ilya A Osterman and A. V. Mardanov and N. V. Ravin},
title = {Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor},
journal = {Nucleic Acids Research},
year = {2020},
volume = {48},
publisher = {Oxford University Press},
month = {sep},
url = {https://doi.org/10.1093%2Fnar%2Fgkaa798},
number = {19},
pages = {10802--10819},
doi = {10.1093/nar/gkaa798}
}
MLA
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MLA Copy
Pletnev, Philipp, et al. “Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor.” Nucleic Acids Research, vol. 48, no. 19, Sep. 2020, pp. 10802-10819. https://doi.org/10.1093%2Fnar%2Fgkaa798.
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